Addgene

We narrowed to 6,927 results for: 11

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GFP-polyQ23-NLS

Plasmid

#231891

Purpose

Expression of non-aggregated HTT partial exon 1 Q23-NLS in the nucleus

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

HTT partial exon 1 Q23-NLS (HTT Human)

Use

Tags

EGFP and SV40 NLS

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-polyQ74-NLS

Plasmid

#231892

Purpose

Forms nuclear HTT partial exon 1 Q74-NLS aggregates

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

HTT partial exon 1 Q74-NLS (HTT Human)

Use

Tags

EGFP and SV40 NLS

Expression

Mammalian

Mutation

CAG repeat expansion

Promoter

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-mproSAAS (27 kDa)

Plasmid

#231635

Purpose

Mammalian expression of a flag-tagged full-length version of the mouse chaperone protein proSAAS.

Depositor

Iris Lindberg

Article

Peinado et al ACS Chem Neurosci. 2022 Jun 1;13(11):165

Insert

Pcsk1N (Pcsk1n Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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aTY50_pPGK1_Pcad_IRES_PuroR_WPRE

Plasmid

#225348

Purpose

Lentiviral vector for Pcad expression

Depositor

Wendell Lim

Article

Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

Insert

Pcad (Cdh3 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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aTY65_pTRE_Wnt3a_IRES_tagBFP2_WPRE

Plasmid

#225349

Purpose

Lentiviral vector for Wnt3a and BFP expression

Depositor

Wendell Lim

Article

Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

Insert

Wnt3a (Wnt3a Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2puro-E-cadherin(Canis)-exon1 6-28 gRNA

Plasmid

#209922

Purpose

A knock-out vector for the dog CDH1

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

A gRNA targeting the dog CDH1 gene.

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2puro-p120catenin(Canis)-exon4 116-138 gRNA

Plasmid

#209923

Purpose

A knock-out vector for the dog CTNND1

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

A gRNA targeting the dog CTNND1 gene.

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-TGFa-mNeonGreen

Plasmid

#209913

Purpose

To monitor the expression of TGFα, the plasmid encodes a recombinant TGFα fused intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

TGFa-mNeonGreen (TGFA Human)

Use

Lentiviral

Tags

mNeonGreen

Expression

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pB-Neo-mCherry-mDYNLL1

Plasmid

#210336

Purpose

Overexpression of mDYNLL1

Depositor

Li Yu

Article

Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

Insert

mDYNLL1 (Dynll1 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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WT_IFL_A28C_R

Plasmid

#200184

Purpose

WT PA14 I-F Leader and A28C Repeat

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Wildtype I-F Leader and a mutant I-F Locus (A28C)

Use

Tags

Expression

Bacterial

Mutation

A28C in the I-F repeat

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xKBstTyrT(CUA)_EF1_sfGFP 102TAG 150TAA

Plasmid

#174895

Purpose

amber and ochre dual suppression reporter sfGFP 102TAG 150 TAA expression, with BstTyr(CUA) amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

102TAG 150TAA in sfGFP

Promoter

EF1

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xMmaPylT(UCA)_EF1_MmaPylRS

Plasmid

#174899

Purpose

Mma PylRS expression for opal suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

Mma PylRS (pylS Methanosarcina mazei)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EF1

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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GFP Cav2.2 W391A pRK5

Plasmid

#206084

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit binding

Depositor

Annette Dolphin

Article

Waithe et al J Biol Chem. 2011 Mar 18;286(11):9598-61

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

GFP

Expression

Mammalian

Mutation

W391A

Promoter

CMV

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA W391A pcDNA3

Plasmid

#206071

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2

Depositor

Annette Dolphin

Article

Waithe et al J Biol Chem. 2011 Mar 18;286(11):9598-61

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

W391A

Promoter

CMV

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-A11(2-88)-Strep

Plasmid

#196214

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-88)-Strep.

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Use

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Mutation

Promoter

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-A11(89-196)

Plasmid

#196215

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(89-196).

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Use

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Mutation

Promoter

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-A11(2-68)-Strep

Plasmid

#196216

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-68)-Strep.

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Use

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Mutation

Promoter

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-A11(2-52)-Strep

Plasmid

#196213

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-52)-Strep.

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Use

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Mutation

Promoter

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot2-del393-429-W556E-dsRNAres_U

Plasmid

#147713

Purpose

Insect Expression of DmNot2-del393-429-W556E-dsRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-del393-429-W556E-dsRNAres (Rga Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot2-del429-453-W556E-dsRNAres_U

Plasmid

#147715

Purpose

Insect Expression of DmNot2-del429-453-W556E-dsRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-del429-453-W556E-dsRNAres (Rga Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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