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MDH1-pre-let-7_PShairpin

Plasmid

#26537

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Aug 31. ():.

Insert

pre-let-7_PS hairpin

Use

Retroviral

Tags

Expression

Mammalian

Mutation

5 nt mutated to make pre-let-7 stem pairing perfe…

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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phRL-TKlk-lin-41_LCS-2U

Plasmid

#26543

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Aug 31. ():.

Insert

lin-41_LCS-2U

Use

Tags

Expression

Mammalian

Mutation

Seed regions of both target sites are mutated.

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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phRL-TKlk-lin-41_LCS-2G

Plasmid

#26544

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Aug 31. ():.

Insert

lin-41_LCS-2G

Use

Tags

Expression

Mammalian

Mutation

Seed regions of both target sites are mutated.

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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RCAS(A) cTbx5 D1 (CT#636)

Plasmid

#13968

Depositor

Cliff Tabin

Article

Rallis et al Development. 2003 Jun . 130(12):2741-51.

Insert

Tbx5 (TBX5 Chicken)

Use

Retroviral; Avian expression

Tags

Expression

Mutation

Amino acids 1-274

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
PPRE X3-TK-luc

Plasmid

#1015

Purpose

PPAR reporter. 3xDR1 sites upstream of a luciferase reporter.

Depositor

Bruce Spiegelman

Article

Kim et al Proc Natl Acad Sci U S A 1998 Apr 14;95(

Insert

PPRE

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2005

Availability

Academic Institutions and Nonprofits only

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GFP-TIS11B

Plasmid

#121157

Purpose

Mammalian expression of GFP tagged TIS11B

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

ZFP36L1 (TIS11B) (ZFP36L1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pBAC-lacZ

Plasmid

#13422

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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CMV-Tol2 transposase

Plasmid

#158774

Purpose

Expression of Tol2 transposase

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

Tol2 tranposase

Use

Tags

Expression

Mammalian

Mutation

Tol2 codon usage frequency well suitable for muri…

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pEYFP-Sec24C

Plasmid

#66608

Purpose

YFP fusion of Sec24C

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

Insert

Sec24C (SEC24C Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pCAMBIA-FLS2prom::FLS2-3xmyc-GFP

Plasmid

#86157

Purpose

FLS2-GFP expresssion

Depositor

Silke Robatzek

Article

Robatzek et al Genes Dev. 2006 Mar 1;20(5):537-42.

Insert

FLS2 (FLS2 Mustard Weed)

Use

Tags

3xmyc and GFP

Expression

Plant

Mutation

Promoter

Available since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-TRE3G-dCas9-HaloTag

Plasmid

#169448

Purpose

expression of dCas9-HaloTag

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

dCas9-HaloTag

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2021

Availability

Academic Institutions and Nonprofits only

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Cytoplasmic PancACe

Plasmid

#215708

Purpose

Expresses acetyl-CoA biosensor in the cytoplasm

Depositor

Katharine Diehl

Article

Smith et al bioRxiv 2023.12.31.573774

Insert

PancACe

Use

Lentiviral

Tags

FLAG, HA, and cytoplasmic localization sequence

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 VDAC1-Linker-GFP

Plasmid

#211734

Purpose

Expresses GFP tagged VDAC1 in mammalian cells. GFP is attached to the C-terminus of VDAC1 with a flexible linker.

Depositor

Roland Malli

Article

Pilic et al bioRxiv 2023.09.08.556841

Insert

Voltage-dependent anion channel 1 (VDAC1 )

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pR-spondin 1

Plasmid

#128844

Purpose

bacterial expression of pR-spondin 1

Depositor

Marc de la Roche

Article

Urbischek et al Sci Rep. 2019 Apr 17;9(1):6193. doi: 10.

Insert

R-spondin 1 (RSPO1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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Mitochondrial PancACe

Plasmid

#215710

Purpose

Expresses acetyl-CoA biosensor in the mitochondria

Depositor

Katharine Diehl

Article

Smith et al bioRxiv 2023.12.31.573774

Insert

PancACe

Use

Lentiviral

Tags

FLAG, HA, and mitochondrial localization sequence…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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MBP-3C-hNorrin(33-133)-1D4_pAcGP67a

Plasmid

#216384

Purpose

Baculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218)

Depositor

William Weis

Article

Bruguera et al bioRxiv 2024.02.03.578714

Insert

Norrin (NDP Human)

Use

Baculovirus

Tags

GP64 signal sequence-MBP-3C and Rho1D4

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV6M-Pak1

Plasmid

#12209

Depositor

Jonathan Chernoff

Article

Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

Insert

Pak1 (PAK1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pHES835

Plasmid

#87946

Purpose

Expresses YFP under control of synthetic promoter containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pZ-YFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

pZ

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-TIS11B

Plasmid

#121140

Purpose

Mammalian expression of mCherry tagged TIS11B

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

ZFP36L1 (TIS11B) (ZFP36L1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pUF-Cas9-pre-sgRNA

Plasmid

#137845

Purpose

Cas9 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

Depositor

Josh Beck

Article

Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 18, 2020

Availability

Academic Institutions and Nonprofits only

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