Addgene

We narrowed to 613 results for: gfp fluorescence reporter

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pCMV-iRFP713-ORP9-PH

Plasmid

#214269

Purpose

Mammalian expression of near-IR fluorescent PI4P reporter iRFP713-ORP9-PH

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

iRFP713-ORP9(1-111) (Osbpl9 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-dTomato-ORP9-PH

Plasmid

#214268

Purpose

Mammalian expression of red fluorescent PI4P reporter dTomato-ORP9-PH

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

dTomato-ORP9(1-111) (Osbpl9 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-mTagBFP2-ORP9-PH

Plasmid

#214267

Purpose

Mammalian expression of blue fluorescent PI4P reporter mTagBFP2-ORP9-PH

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

mTagBFP2-ORP9(1-111) (Osbpl9 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-mTagBFP2-PLCδ-PH

Plasmid

#214275

Purpose

Mammalian expression of blue fluorescent PI(4,5)P2 reporter mTagBFP2-PLCδ-PH

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

mTagBFP2-PLCδ(1-175) (PLCD1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC591 - pCMV Mem-iRFP

Plasmid

#59134

Purpose

A plasmid that express membrane-targeted iRFP under the CMV promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

Membrane-localized Infrared Fluorescent Protein

Use

Tags

Palmitylation signal

Expression

Mammalian

Mutation

Promoter

CMV-IE

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pTH100

Plasmid

#84458

Purpose

pJB38-NWMN29-30 + SarA_P1-sGFP-Term

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

SarA_P1-sGFP from pCM29

Use

Tags

6xHis (on sfGFP)

Expression

Mutation

Promoter

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pSlow-FT-N1

Plasmid

#31912

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Insert

Slow-FT

Use

Tags

Expression

Mammalian

Mutation

M18V/E30V/K69R/L84W/A179V compared to mCherry (bu…

Promoter

CMV

Available since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pFast-FT-N1

Plasmid

#31910

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Insert

Fast-FT

Use

Tags

Expression

Mammalian

Mutation

M18V/E34K/K69R/L84W/S149T/A224S compared to mCher…

Promoter

CMV

Available since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pMedium-FT-N1

Plasmid

#31911

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Insert

Medium-FT

Use

Tags

Expression

Mammalian

Mutation

M18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…

Promoter

CMV

Available since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pMPRA1-CMV-Clover

Plasmid

#53538

Purpose

Fluorescent reporter control for pMPRA1

Depositor

Tarjei Mikkelsen

Article

Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

Insert

Clover GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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pMPRA2-CMV-Clover

Plasmid

#53539

Purpose

Fluorescent reporter control for pMPRA2

Depositor

Tarjei Mikkelsen

Article

Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

Insert

Clover GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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pMPRA3-CMV-Clover

Plasmid

#53540

Purpose

Fluorescent reporter control for pMPRA3

Depositor

Tarjei Mikkelsen

Article

Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

Insert

Clover GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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Cre Stoplight

Plasmid

#45150

Depositor

Thomas Hughes

Article

Yang et al Biotechniques. 2001 Nov;31(5):1036, 1038

Insert

Cre Stoplight

Use

Tags

DsRed and EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG-2TAG

Plasmid

#158138

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (2 TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Use

Tags

Expression

Yeast

Mutation

Cloned in a TAG codon at the first serine residue…

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pTriEx4-H6-FGB

Plasmid

#49203

Purpose

Fluorescent reporter for cGMP

Depositor

John Garthwaite

Article

Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

Insert

H6-FGB

Use

Tags

His tag + Other tags

Expression

Bacterial, Insect, and Mamm…

Mutation

None

Promoter

CMV

Available since

Dec. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pLV 4xSTAR-mScarletI-NLS-blast

Plasmid

#136260

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

mScarletI-NLS

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLV 4xSTAR-sTomato-NLS-blast

Plasmid

#136259

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

sTomato-NLS

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLV 4xSTAR-mNeonGreen-NLS-blast

Plasmid

#136258

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

mNeonGreen-NLS

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLV 4xSTAR-mTFP-NLS-blast

Plasmid

#136257

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

mTFP-NLS

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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EF.hICN1

Plasmid

#113715

Purpose

Constitutive expression of hICN1, e.g. for hedgehog-induction. Based on addgene #17623, with CMV.GFP removed.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Insert

None

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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