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pAW092
Plasmid#113250PurposeE. coli expression vector for His-MBP-S. pyogenes Cas1 H205ADepositorInsertS. pyogenes Cas1
UseTags6xHis-MBPExpressionBacterialMutationH205APromoterT7Available sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
Halo-TMD
Plasmid#195347PurposeFluorescent labeling for cell membranesDepositorInsertHaloTag
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKDC.109
Plasmid#227189PurposeT7 IPTG-driven Cas9DepositorInsertSpyCas9
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
4xNLS-pMJ915v2
Plasmid#88917PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
4xNLS-pMJ915v2-sfGFP
Plasmid#88921PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
2xNLS-pMJ915v2
Plasmid#88916PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
1xNLS-pMJ915v2
Plasmid#88915PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertCas9
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMJ915v2 + sfGFP
Plasmid#88918PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-ELP
Plasmid#112630PurposeTwo SpyCatchers linked by an elastin-like polypeptide with a C-terminal SnoopCatcherDepositorInsertTriCatcher-ELP
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT5Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
sgRNA AAVS1
Plasmid#128119PurposeCo-expresses sgRNA AAVS1, SpyCas9 scaffold (F+E) and GFP (transfection marker)DepositorInsertsgRNA AAVS1 + GFP
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterRSV/U6Available sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
7xNLS-pMJ915v2-sfGFP
Plasmid#88922PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEXndoS(E235Q)
Plasmid#44656DepositorInsertndoS (ndoS Streptococcus pyogenes)
UseTagsGstExpressionBacterialMutationChanged Glutamic acid 235 to GlutaminePromotertacAvailable sinceMay 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
Cas-Acr (insertion 5)
Plasmid#128116PurposeExpresses SpyCas9 fused to attenuated AcrIIA4 (insertion 5)DepositorInsertCascAID (insertion 5)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cas-Acr (N39A)
Plasmid#128117PurposeExpresses SpyCas9 fused to attenuated AcrIIA4 (N39A)DepositorInsertCascAID (N39A)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cas-Acr (wtAcrIIA4)
Plasmid#128115PurposeExpresses SpyCas9 fused to wild-type AcrIIA4DepositorInsertCascAID (wtAcrIIA4)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgRNA EMX1
Plasmid#128121PurposeCo-expresses sgRNA EMX1, SpyCas9 scaffold (F+E) and GFP (transfection marker)DepositorInsertsgRNA EMX1 + GFP
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterRSV/U6Available sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
1xNLS-pMJ915v2-sfGFP
Plasmid#88919PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
2xNLS-pMJ915v2-sfGFP
Plasmid#88920PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
sgRNA HBB
Plasmid#128120PurposeCo-expresses sgRNA HBB, SpyCas9 scaffold (F+E) and GFP (transfection marker)DepositorInsertsgRNA HBB + GFP
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterRSV/U6Available sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only
