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Plasmid#61660Purpose10nm SAH scaffold with EF Hand and split GFP attachment sitesDepositorInsertSNAP-EF(N)-10nmSAH-GFP(1-10)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA0055
Plasmid#131774PurposeGuide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles.DepositorInsert20mer CASS5a guide (gCASS5a) and 5' sgRNA
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
secCitrine
Plasmid#181964PurposeBasic chitinase signal peptide fused to Citrine, under control of MUM4_1.5kb promoterDepositorInsertsignal peptide sequence from basic chitinase
UseTagsCitrine YFPExpressionPlantMutationPromoter1.5 kb MUM4 promoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
SNAP-EF(N)-60nmSAH-GFP(1-10)
Plasmid#61663Purpose2x-30nm SAH scaffold with EF Hand and split GFP attachment sitesDepositorInsertSNAP-EF(N)-60nmSAH-GFP(1-10)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDBTrp-LexABD-CRY2FL
Plasmid#78210PurposeUse with Addgene 40615 for blue light transcription control in yeastDepositorInsertLexABD-CRY2 (CRY2 Mustard Weed)
UseTagslexA binding domainExpressionYeastMutationcontains endogenous CRY2 NLSPromoterADH1Available sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
SNAP-EF(N)-5nmSAH-GFP(1-10)
Plasmid#61659Purpose5nm SAH scaffold with EF Hand and split GFP attachment sitesDepositorInsertSNAP-EF(N)-5nmSAH-GFP(1-10)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
p alpha-actinin:GAL4/UAS:TdTomato
Plasmid#161712PurposeExpress TdTomato through amplification of GAL4/UAS systemDepositorInsertTdTomato, GAL4
UseXenopus laevis expressionTagsTdTomatoExpressionMammalianMutationPromoteractin-GAL4, UASAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
gRD21A-mRFP
Plasmid#181959PurposeRD21A protein tagged with mRFP, under control of native promoterDepositorInsertRD21A genomic sequence including 2kb upstream of the ATG
UseTagsmRFPExpressionPlantMutationPromoterRD21A nativeAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
SNAP-EF(N)-30nmSAH-GFP(1-10)
Plasmid#61662Purpose30nm SAH scaffold with EF Hand and split GFP attachment sitesDepositorInsertSNAP-EF(N)-30nmSAH-GFP(1-10)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUASp-GBP-Tbro
Plasmid#179995PurposeExpresses two tandem copies of the GFP nanobody fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system. The UASp version is ideal for expression in the germline.DepositorInsertGBP nanobody
UseTagsTrboExpressionInsectMutationPromoterUAS-Gal4Available sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFM-NbrWT
Plasmid#40830PurposeExpresses Flag, Myc tagged Nbr in Drosophila cellsDepositorInsertNbr (Nbr Fly)
UseTags3xFLAG and 6xMycExpressionInsectMutationPromoterActin5CAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1 human APC-B,C (1133–1189)
Plasmid#132630Purposeexpresses human APC-B,C (1133–1189) in E. coliDepositorInsertAPC-B,C (1133–1189)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cav1-miniSOG
Plasmid#67667PurposeMammalian expression vector driving dog Caveolin1-minSOGDepositorInsertDog Cav1-miniSOG
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCF823_EFS-Cas9-P2A-Puro
Plasmid#211645PurposeEFS-Cas9-P2A-PuroDepositorInsertCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF226_EFS-Cas9-P2A-Puro
Plasmid#211644PurposeEFS-Cas9-P2A-PuroDepositorInsertCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSCV_JAK3_IRES_BFP
Plasmid#206291PurposeExpresses the wild type form of human JAK3.DepositorInsertJAK3 (JAK3 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-IC2N-dCer3
Plasmid#191325PurposeChronically inhibit dynein by expressing the dominant negative acting N-terminal domain of the dynein intermediate chain (IC2N)DepositorInsertIC2N-dCer-FRB (Dync1i2 Mouse)
UseTagsExpressionMammalianMutationaa 1-237PromoterAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
MX01
Plasmid#158624PurposeGATEWAY and BacMam compatible mammalian expression vector with eGFP expression reporter (eGFP is expressed by same promotor but as a separate polypeptide chain).DepositorTypeEmpty backboneUseGateway destination vector ; bacmam acceptor vect…TagsP2AExpressionMammalianMutationPromoterCMV-IEAvailable sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSF-1b-S7-S83C/Δ157-178
Plasmid#128592PurposeExpresses tagless S7-S83C, Δ157-178 mutant 30S ribosomal protein in E.coliDepositorInsertS7 E.coli ribosomal protein (rpsG E.coli)
UseTagsExpressionBacterialMutationS83C, Δ157-178PromoterT7Available sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-CfANLN_sgRNA
Plasmid#183880PurposepX459V2.0-HypaCas9 plasmid with cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells.DepositorInsertCanis familiaris ANLN sgRNA spacer
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only