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pCOND (Hyg)
Plasmid#110093PurposeSuicide vector based on the TetR/Pip OFF system.DepositorTypeEmpty backboneUseBacterial knockout generationTagsExpressionMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCC336
Plasmid#110167PurposeminiMos vector expresses ERKKTR(S43E,T55E,T62E)-mClover and mCherry-H2B in C. elegans VPCs; use as control for pCC324 (Contains Neomycin resistance)DepositorInsertERK-KTR(S43E,T55E,T62E)-mClover-T2A-mCherry-H2B
UseMinimos transposonTagsmCherry and mCloverExpressionWormMutationPhospho-acceptor sites mutated: S43E, T55E, T62E.…Promoterlin-31 promoter/enhancerAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCC335
Plasmid#110166PurposeminiMos vector expresses ERKKTR(S43A,T55A,T62A)-mClover and mCherry-H2B in C. elegans VPCs; use as control for pCC324 (Contains Neomycin resistance)DepositorInsertERK-KTR(S43A,T55A,T62A)-mClover-T2A-mCherry-H2B
UseMinimos transposonTagsmCherry and mCloverExpressionWormMutationPhospho-acceptor sites mutated: S43A, T55A, T62A.…Promoterlin-31 promoter/enhancerAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
mature ISG15 C-term. domain (79-157)
Plasmid#110764PurposeBacterial expression of proISG15 C-term. domainDepositorInsertISG15 (ISG15 Human)
UseTagsN-His6-3CExpressionBacterialMutationPromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_RPA1_RepA-N
Plasmid#110032PurposeProtein expression and purification of RPA1_RepA-NDepositorInsertRPA1_RepA-N (RPA1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBTK601
Plasmid#110607PurposeBTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)DepositorInsertCas9
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PFN2_Profilin
Plasmid#110007PurposeProtein expression and purification of PFN2_ProfilinDepositorInsertPFN2_Profilin (PFN2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET29b-pduCDE
Plasmid#110138PurposeExpresses B12-dependent propanediol dehydratase in E. coli BL21(DE3)DepositorUseTagsExpressionBacterialMutationPromoterT7Available sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Designed VEGF-binding scFv G6des13
Plasmid#110213PurposeYeast Surface Display of G6des13DepositorInsertG6des13
UseTagscmyc tagExpressionYeastMutationL A43P, L Q89L, L T94D, L P95T, H Y59H, H Y95FPromoterAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
ECFP-Rab5C-S35N
Plasmid#110504PurposeDominant NegativeDepositorInsertRab5C-S35N (RAB5C Human)
UseTagsECFPExpressionMammalianMutationS35NPromoterAvailable sinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ACR_Trypsin
Plasmid#110042PurposeProtein expression and purification of ACR_TrypsinDepositorInsertACR_Trypsin (ACR Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCZGY2162
Plasmid#110136PurposeEpidermal Mitochondrial-tagged Florescent Calcium SensorDepositorInsertmito::GCaMP3
UseTagsmito::GCaMP3ExpressionWormMutationPromoterPcol-19Available sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#59
Plasmid#110929PurposeExpresses proteins pan-neuronally in C. elegans (entire nervous system)DepositorInsertsrab-3 promoter
unc-54 3' control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
proISG15 C-term. domain (79-165)
Plasmid#110763PurposeBacterial expression of proISG15 C-term. domainDepositorInsertISG15 (ISG15 Human)
UseTagsN-His6-3CExpressionBacterialMutationPromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-SFXN2
Plasmid#110636PurposeRetroviral expression of Flag-SFXN2 in mammalian cellsDepositorInsertSFXN2 (SFXN2 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DNM2_Dynamin-N
Plasmid#110008PurposeProtein expression and purification of DNM2_Dynamin-NDepositorInsertDNM2_Dynamin-N (DNM2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBTK300
Plasmid#110592PurposeBTK Type 4 terminator for golden gate assemblyDepositorInsertrpoC
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMINTC3GH (Apr)
Plasmid#110078PurposeAnalogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-SFXN4
Plasmid#110638PurposeRetroviral expression of Flag-SFXN4 in mammalian cellsDepositorInsertSFXN4 (SFXN4 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only
