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Showing: 951 - 1000 of 23234 results

  6. pZS1[FruR-T]

    Plasmid
    #60752
    Purpose
    Contains PIq driving expression FruR-T, the Fructose inducible chimera with the TAN DBD.
    Depositor
    Matthew Bennett
    Article
    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.
    Inserts
    FruR-T
    FruR-T
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Chimeric LacI/GalR repressor with the TAN DBD and…
    Promoter
    Available since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    60752-plasmid-map-sequence-id-97733

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  9. pCDF-Az

    Plasmid
    #197101
    Purpose
    To express the azidophenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into azidophenylalanine
    Depositor
    Kensaku Sakamoto
    Article
    Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20
    Insert
    TyrRS variant, amber suppressor tRNA, kanamycin resistance gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Thr32, Asn107, Pro158, Leu159, Gln162, Arg286 (Ty…
    Promoter
    Available since
    June 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    197101-plasmid-map-sequence-id-390718

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  10. pCAT100

    Plasmid
    #180512
    Purpose
    Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    PlmCasX with DpbCasX R1 loop
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180512-plasmid-map-sequence-id-359730

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  11. pCAT527

    Plasmid
    #180514
    Purpose
    Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, mNeonGreen, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    PlmCasX with DpbCasX R1 loop-2A-mNeonGreen
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180514-plasmid-map-sequence-id-359727

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  12. pZS1[TreR-T]

    Plasmid
    #60754
    Purpose
    Contains PI driving expression TreR-T, the Trehalose inducible chimera with the TAN DBD.
    Depositor
    Matthew Bennett
    Article
    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.
    Inserts
    TreR-T
    GalS-T
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Chimeric LacI/GalR repressor with the TAN DBD and…
    Promoter
    Available since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    60754-plasmid-map-sequence-id-97736

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  13. pZS1[GalS-T]

    Plasmid
    #60753
    Purpose
    Contains PIq driving expression GalS-T, the Fucose inducible chimera with the TAN DBD.
    Depositor
    Matthew Bennett
    Article
    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.
    Inserts
    GalS-T
    GalS-T
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Chimeric LacI/GalR repressor with the TAN DBD and…
    Promoter
    Available since
    July 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    60753-plasmid-map-sequence-id-97735

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  16. pcU6_3 MS2 sgRNA

    Plasmid
    #92394
    Purpose
    Chick-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter, with tracrRNA scaffold containing stem loops allowing binding of bacteriophage MS2 coat protein MCP
    Depositor
    Tatjana Sauka-Spengler
    Article
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Insert
    chick U6.3 promoter and gRNA cloning cassette including MS2 stem loops
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    chick U6.3
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    92394-plasmid-map-sequence-id-191866

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  20. CmCitrine Lck w/ NosUTRs

    Plasmid
    #66785
    Purpose
    expression of fluorescent membranes in sea urchin primordial germ cells
    Depositor
    Amro Hamdoun
    Article
    Campanale et al Dev Dyn. 2014 Jul;243(7):917-27. doi: 10
    Insert
    Lck (LCK Human)
    Use
    Sea urchin, zebrafish, xenopus
    Tags
    3' UTR from sea urchin Nanos, 5' UTR fr…
    Expression
    Mutation
    Tandem repeat of LCK the N-terminal membrane targ…
    Promoter
    T7
    Available since
    July 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  21. pHOT-P2Y2R

    Plasmid
    #159108
    Purpose
    entiviral vector encoding P2Y purinoceptor 2 (P2Y2R), a high affinity G-protein coupled receptor that mobilizes Ca2+ from intracellular stores upon binding extracellular ATP.48,49 By using the Gibson
    Depositor
    Fabio Mammano
    Article
    Mazzarda et al Lab Chip. 2020 Jul 23. doi: 10.1039/d0lc
    Insert
    P2Y2R
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    UBC
    Available since
    Jan. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    159108-plasmid-map-sequence-id-318664

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  23. pTYB1-T4denV

    Plasmid
    #228216
    Purpose
    Expression in BL21(DE3) of T4 endonuclease V N-glycosylase UV repair enzyme
    Depositor
    Stephen Benkovic
    Insert
    denV
    Use
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    228216-plasmid-map-sequence-id-455996

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  24. pET-Impact-T4gp41

    Plasmid
    #228207
    Purpose
    Expression in BL21(DE3) of T4 helicase
    Depositor
    Stephen Benkovic
    Insert
    41 (41 Escherichia phage T4)
    Use
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    228207-plasmid-map-sequence-id-453945

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  25. pET-Impact-T4gp59

    Plasmid
    #228206
    Purpose
    Expression in BL21(DE3) of WT T4 helicase loader
    Depositor
    Stephen Benkovic
    Insert
    59 (59 Escherichia phage T4)
    Use
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    228206-plasmid-map-sequence-id-453944

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  26. pTYB1-T4uvsX

    Plasmid
    #228213
    Purpose
    Expression in BL21(DE3) of T4 recombinase
    Depositor
    Stephen Benkovic
    Insert
    uvsX (uvsX Escherichia phage T4)
    Use
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    228213-plasmid-map-sequence-id-455994

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  27. pTBY1-T4uvsY

    Plasmid
    #228214
    Purpose
    Expression in BL21(DE3) of T4 UvsY-like recombination mediator
    Depositor
    Stephen Benkovic
    Insert
    uvsY (uvsY Escherichia phage T4)
    Use
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    228214-plasmid-map-sequence-id-455995

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  28. pET-Impact-T4gp43(exo-)

    Plasmid
    #228202
    Purpose
    Expression in BL21(DE3) of 3' to 5' exonuclease-deficient T4 DNA polymerase D219A
    Depositor
    Stephen Benkovic
    Insert
    43
    Use
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Mutated aspartate 219 to alanine (D219A), elimina…
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    228202-plasmid-map-sequence-id-453943

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  30. pCFD3-dUG-DH1

    Plasmid
    #138963
    Purpose
    Used to edit the endogenous Drosophila kkv gene to encode a fluorescent Chitin Synthase.
    Depositor
    Paul Adler
    Article
    Adler et al bioRxiv 2020
    Insert
    DH1 oligo
    Use
    CRISPR
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    138963-plasmid-map-sequence-id-274780

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  38. pJK057

    Plasmid
    #75036
    Purpose
    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase, N-terminal His6 (H6EcPurE1) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)
    Depositor
    JoAnne Stubbe, T. J. Kappock
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase (purK Escherichia coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes residues 2-7 to His (SSRNNP>HHHHHH)
    Promoter
    T7 and n/a
    Available since
    Oct. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    75036-plasmid-map-sequence-id-142299

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  39. pEG03

    Plasmid
    #154138
    Purpose
    This plasmid carries fucO and aldA under the control of P_gyrA promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.
    Depositor
    Kang Zhou
    Article
    Panda et al Biochemical Engineering Journal
    Insert
    fucO, aldA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P_gyrA
    Available since
    June 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    154138-plasmid-map-sequence-id-332116

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  40. pKSCTMF

    Plasmid
    #85073
    Purpose
    Gene insertion/deletion in Pichia pastoris
    Depositor
    Sheng Yang
    Article
    Yang et al FEMS Yeast Res. 2009 Jun;9(4):600-9. doi
    Insert
    AOX1promoter - mazF - AOX1 tt- Zeocin R gene
    Use
    Gene insertion/deletion in pichia pastoris
    Tags
    Expression
    Mutation
    Promoter
    pAOX1
    Available since
    March 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    85073-plasmid-map-sequence-id-174101

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  43. pEG03'

    Plasmid
    #162578
    Purpose
    This plasmid carries fucO and aldA under the control of P_gyrA promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.
    Depositor
    Kang Zhou
    Article
    Panda et al Biochemical Engineering Journal
    Insert
    fucO, aldA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P_gyrA
    Available since
    June 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    162578-plasmid-map-sequence-id-332111

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  44. pPV033

    Plasmid
    #178359
    Purpose
    Targeted gene knock-in plasmid for Schizophyllum commune
    Depositor
    Han Wösten
    Article
    Vonk et al Open Res Europe 2021, 1:140
    Inserts
    Left flank
    nour_cassette
    Right flank
    Use
    Fungal homologous recombination plasmid
    Tags
    Expression
    Mutation
    Promoter
    S. commune gpd and n.a.
    Available since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    178359-plasmid-map-sequence-id-350051

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  46. pEG01

    Plasmid
    #154136
    Purpose
    This plasmid carries fucO and aldA under the control of P_BAD promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.
    Depositor
    Kang Zhou
    Article
    Panda et al Biochemical Engineering Journal
    Insert
    fucO, aldA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P_BAD
    Available since
    June 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    154136-plasmid-map-sequence-id-332112

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  47. pEG02

    Plasmid
    #154137
    Purpose
    This plasmid carries fucO and aldA under the control of P_thrC3 promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.
    Depositor
    Kang Zhou
    Article
    Panda et al Biochemical Engineering Journal
    Insert
    fucO, aldA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P_thrC3
    Available since
    June 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    154137-plasmid-map-sequence-id-332114

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Showing: 951 - 1000 of 23234 results