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Plasmid#182346PurposePacI-flanked CAG-LSL expression cassette with a FNF for positive selectionDepositorInserttdTomato
UseMouse Targeting; Flp / frtTagsExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
mT2A-sfGFP-linker-iCre-ERT2-FNF
Plasmid#182341PurposeRecombineering cassette plasmid for T2A-sfGFP-linker-iCre-ERT2-FNFDepositorInsertT2A-sfGFP-linker-iCre-ERT2-FNF
UseMouse Targeting; Flp / frtTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-frt-DIO-hM3D(Gq)-mCherry
Plasmid#217397PurposeExpresses the hM3D(Gq) excitatory DREADD fused to mCherry, in a Cre-ON and FLP-OFF dependent manner, under the control of hSyn promoterDepositorInserthM3D(Gq)-mCherry
UseAAVTagsmCherryExpressionMammalianMutationPromoterhuman Synapsin 1Available sinceMay 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-frt-DIO-hM4D(Gi)-mCherry
Plasmid#217398PurposeExpresses the hM4D(Gi) inhibitory DREADD fused to mCherry, in a Cre-ON and FLP-OFF dependent manner, under the control of hSyn promoterDepositorInserthM4D(Gi)-mCherry
UseAAVTagsmCherryExpressionMammalianMutationPromoterhuman Synapsin 1Available sinceMay 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDRAV-2
Plasmid#26747DepositorTypeEmpty backboneUseCre/Lox; Flp/frt; phic31/attb-attpTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDRAV-1
Plasmid#26746DepositorTypeEmpty backboneUseCre/Lox; Flp/frt; phic31/attb-attpTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDRAV-3
Plasmid#26748DepositorTypeEmpty backboneUseCre/Lox; Flp/frt; phic31/attb-attpTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDRAV-4
Plasmid#26749DepositorTypeEmpty backboneUseCre/Lox; Flp/frt; phic31/attb-attpTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/Flag-tGFP-Cryz
Plasmid#110378PurposeMammalian expression of turboGFP-CRYZ with FLAG tag, Flp-In T-Rex systemDepositorInsertcrystallin zeta (CRYZ Human)
UseTagsFLAG and tGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
PGK-mCherry
Plasmid#236119PurposeFluorescent transfection marker encoding mCherry under PGK promoterDepositorInsertmCherry
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAFNF-EGFP
Plasmid#163609PurposeExpresses EGFP Flp-dependently in mammalian cellsDepositorInsertEgfp
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT03-Apr
Plasmid#154756PurposeFor curing of the resistance marker of RecET recombinase system in Acinetobacter baumannii, AprarDepositorInsertFLP recombinase
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
LCMV:ECFP(loxP)(FRT)EYFP
Plasmid#31304DepositorInsertECFP(loxP)(FRT)EYFP
UseCre/Lox; Flp/frtTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pE_Cre_lambdaCos
Plasmid#173785PurposeThe pE_Cre_lambdaCos plasmid is used to remove the antibiotic resistance cassette from pIT5 integrants.DepositorInsertCre
UseCre/Lox and Synthetic BiologyTagsExpressionMutationS102Y and S279Y- please see depositor commentsPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/Flag-tGFP-HuR.wt
Plasmid#110376PurposeMammalian expression of turboGFP-HuR (wild type) with FLAG tag, Flp-In T-Rex systemDepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseTagsFLAG and tGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSH87
Plasmid#127705PurposePflp-13::gfp1-10DepositorInsertGFP1-10
UseTagsExpressionWormMutationPromoterflp-13Available sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAFNF-Rac1
Plasmid#163607PurposeExpresses Rac1 Flp-dependently in mammalian cellsDepositorInsertRac1 (Rac1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
LCMV:ECFP(FRT)EYFP
Plasmid#31307DepositorInsertECFP(FRT)EYFP
UseFlp/frtTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJPC432
Plasmid#234528PurposeFLP Recombinase switch; plant T-DNADepositorInsertRUBY
UseTagsExpressionPlantMutationnonePromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
loxP-T2A-Myr-DeadCasp8-ERT2-rox-FNF-loxP-T2A-Myr-Casp8-ERT2-rox
Plasmid#182360PurposeShuttle vector for T2A-Myr-DeadCasp8-ERT2 and T2A-Myr-Casp8-ERT2DepositorInsertMyr-DeadCasp8-ERT2 and Myr-Casp8-ERT2
UseCre/Lox, Mouse Targeting, and Synthetic Biology ;…TagsExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAFNF-Rhoa
Plasmid#163605PurposeExpresses RhoA Flp-dependently in mammalian cellsDepositorInsertRhoa (Rhoa Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAFNF-LifeAct-EGFP
Plasmid#163608PurposeExpresses LifeAct fused to EGFP Flp-dependently in mammalian cellsDepositorInsertLifeAct
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSIJ8
Plasmid#68122PurposeTemperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expressionDepositorInsertsflp recombinase
rhaS
rhaR
UseTagsExpressionBacterialMutationPromoterrhamnoseAvailable sinceDec. 8, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDIRE
Plasmid#26745DepositorInsertiCre expression cassette
UseCre/Lox; Flp/frtTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato
Plasmid#60658PurposeFlp-dependent expression of mNaChBac-T2A-tdTomatoDepositorHas ServiceAAV8InsertFLEX-mNaChBac-T2A-tdTomato
UseAAVTagsExpressionMutationPromoterEF1αAvailable sinceNov. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-F-FLEX-mNaChBacMut-T2A-tdTomato
Plasmid#60660PurposeFlp-dependent expression of mNaChBacMut-T2A-tdTomatoDepositorInsertFLEX-mNaChBacMut-T2A-tdTomato
UseAAVTagsExpressionMutationE191KPromoterEF1αAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Fon/ConN2cG
Plasmid#234709PurposepAAV transfer plasmid with Ef1a promoter driving Cre- and Flp-dependent expression of G protein for rabies N2c delta GDepositorInsertFon Con N2cG
UseAAVTagsExpressionMammalianMutationPromoterEf1aAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
Double UP mNeon to mScarlet FRT
Plasmid#141111PurposeNatively produces mNeonGreen, in the presence of Flp or Flpe will be recombined to produce mScarlet.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-F-FLEX- Kir2.1-T2A-tdTomato
Plasmid#60661PurposeFlp-dependent expression of Kir2.1-T2A-tdTomatoDepositorInsertFLEX- Kir2.1-T2A-tdTomato
UseAAVTagsExpressionMutationPromoterEF1αAvailable sinceNov. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCALNL-DsRed
Plasmid#13769PurposeCre recombinase-dependent expression of DsRed, i.e. DsRed is only expressed in the presence of Cre due to a transcriptional stop in between the loxP sites.DepositorInsertDsRed2
UseCre/Lox; Flp/frtTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT-TO-EGFP-UPF1-WT
Plasmid#136000PurposeDOX-inducible WT UPF1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex SystemDepositorInsertUPF1
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pV1200
Plasmid#111429PurposeCaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1 - Recyclable for serial mutagenesis (Sap2-FLP)DepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
FR_luc
Plasmid#31098DepositorInsertfirefly luciferase
UseFlp/frtTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBW820_pCAG-pExpr-FULL-ADDER
Plasmid#87552PurposeEncodes full adder logic in response to Cre, Flp and VCre recombinasesDepositorInsertEGFP, mCherry
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_eGFP-pScaE
Plasmid#228826PurposeFor the expression of the passenger domain of the Orientia tsutsugamushi protein ScaE in HeLa Flp-In cellsDepositorInsertScaE
UseTagseGFPExpressionMammalianMutationaa 49-494PromoterAvailable sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_eGFP-pScaD
Plasmid#228825PurposeFor the expression of the passenger domain of the Orientia tsutsugamushi protein ScaD in HeLa Flp-In cellsDepositorInsertScaD
UseTagseGFPExpressionMammalianMutationaa 30-700PromoterAvailable sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_eGFP-pScaC
Plasmid#228824PurposeFor the expression of the passenger domain of the Orientia tsutsugamushi protein ScaC in HeLa Flp-In cellsDepositorInsertScaC
UseTagseGFPExpressionMammalianMutationaa 33-223PromoterAvailable sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_eGFP-pScaA
Plasmid#228823PurposeFor the expression of the passenger domain of the Orientia tsutsugamushi protein ScaA in HeLa Flp-In cellsDepositorInsertScaA
UseTagseGFPExpressionMammalianMutationaa 25-1216PromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT-TO-EGFP-UPF1-R615A
Plasmid#136001PurposeDOX-inducible R615A UPF1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex SystemDepositorInsertUPF1
UseTagsEGFPExpressionMammalianMutationR615APromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT-TO-EGFP-UPF1-DEAA
Plasmid#136002PurposeDOX-inducible DEAA UPF1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex SystemDepositorInsertUPF1
UseTagsEGFPExpressionMammalianMutationDE647AAPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only