167,948 results

1. LentiCRISPRv2-mCherry

   Plasmid

   #99154

   Purpose

   Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.

   Depositor

   Agata Smogorzewska

   Inserts

   Cas9
   mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EFS-NS

   Available Since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV9)

   Viral Prep

   #44362-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-hSyn-DIO-hM4D(Gi)-mCherry (#44362). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM4D(Gi)-mCherry plasmid DNA. hSyn-driven, Cre-dependent, hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Article

   Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

   Promoter

   Syn

   Tags

   mCherry (Cre-dependent)

   Available Since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

3. pLKO.1 - TRC cloning vector

   Plasmid

   #10878

   Purpose

   3rd generation transfer plasmid

   Depositor

   David Root

   Article

   Moffat et al Cell. 2006 Mar 24. 124(6):1283-98.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV8)

   Viral Prep

   #44362-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-hSyn-DIO-hM4D(Gi)-mCherry (#44362). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM4D(Gi)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Article

   Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

   Promoter

   Syn

   Tags

   mCherry (Cre-dependent)

   Available Since

   Sept. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pNP3386 [For use in genome insertion and off-target assessment]

   Plasmid

   #247258

   Purpose

   Example plasmid for bridge recombinase mediated insertion and off-target assessment with puromycin selection — must clone in UMI

   Depositor

   Patrick Hsu

   Article

   Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

   Insert

   ISCro4 Recognition Site-UMI-Ef1a-PuroR-P2A-mCherry (Plasmid for Insertion Site Mapping [Example, Clone in Sequence + UMI])
   

   Expression

   Mammalian

   Available Since

   Oct. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV2/2

   Plasmid

   #232199

   Purpose

   AAV packaging plasmid expressing AAV2 Rep and capsid proteins

   Depositor

   Addgene Research Program, Melina Fan

   Insert

   Rep2/Cap2
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Sept. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pCMV-PE6d

   Plasmid

   #207854

   Purpose

   Mammalian expression of PE6d prime editor

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   PE6d
   

   Tags

   SV40 bpNLS and SV40 bpNLS, c-Myc NLS

   Expression

   Mammalian

   Mutation

   M-MLVRTDRNAseHrt(T128N, V223Y, D200C)

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. LV_EF1a_SV40_Large_T_Antigen-P2A-Hygro_Barcode

   Plasmid

   #170255

   Purpose

   Barcoded lentiviral vector to express SV40 Large T Antigen in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   SV40 Large T Antigen
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1a-Cre (AAV Retrograde)

   Viral Prep

   #55636-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pAAV-EF1a-Cre (#55636). In addition to the viral particles, you will also receive purified pAAV-EF1a-Cre plasmid DNA. EF1a-driven expression of Cre recombinase. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Promoter

   EF1a

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

10. lentiCas9-Blast (Lentiviral Prep)

    Viral Prep

    #52962-LV

    Purpose

    Ready-to-use Lentiviral Prep particles produced from lentiCas9-Blast (#52962). In addition to the viral particles, you will also receive purified lentiCas9-Blast plasmid DNA. Lentiviral particles carrying Cas9 and blasticidin resistance. This virus is used to make stable cell lines expressing Cas9.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Promoter

    EFS-NS

    Available Since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-FLEX-tdTomato (AAV9)

    Viral Prep

    #28306-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. CAG-driven, Cre-dependent tdTomato expression control. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Promoter

    CAG

    Tags

    tdTomato (Cre-dependent)

    Available Since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

12. pCMV-PEmax-P2A-GFP

    Plasmid

    #180020

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax-P2A-EGFP
    

    Tags

    P2A-EGFP, SV40 bpNLS, and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-hChR2(H134R)-EYFP (AAV5)

    Viral Prep

    #26973-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-hSyn-hChR2(H134R)-EYFP (#26973). In addition to the viral particles, you will also receive purified pAAV-hSyn-hChR2(H134R)-EYFP plasmid DNA. hSyn-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    Syn

    Tags

    EYFP

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

14. pLenti CMV GFP Puro (658-5)

    Plasmid

    #17448

    Purpose

    3rd gen lentiviral eGFP expression vector, CMV promoter, Puro

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Insert

    GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPRia-v2

    Plasmid

    #84832

    Purpose

    CRISPRi/a V2 library parental plasmid

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Inserts

    sgRNA
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV2/5

    Plasmid

    #232922

    Purpose

    AAV packaging plasmid expressing AAV2 Rep protein and AAV5 capsid protein

    Depositor

    Addgene Research Program, Melina Fan

    Insert

    Rep2/Cap5
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Sept. 17, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pAAV2/11

    Plasmid

    #240486

    Purpose

    AAV packaging plasmid expressing AAV2 Rep protein and AAV11 capsid protein

    Depositor

    Meghan Rego, Addgene Research Program

    Insert

    AAV2 Rep and AAV11 Cap
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    July 25, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pAAV2/7

    Plasmid

    #112863

    Purpose

    AAV packaging plasmid expressing Rep/Cap genes

    Depositor

    James M. Wilson

    Insert

    Rep2/Cap7
    

    Use

    AAV

    Promoter

    Truncated P5

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. B95(DE3) ΔA ΔfabR ΔserB

    Bacterial Strain

    #197655

    Purpose

    This strain is used for expressing phosphoserine-containing proteins using genetic code expansion without buildup of prematurely truncated protein.

    Depositor

    Richard Cooley, Ryan Mehl

    Article

    Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

    Bacterial Resistance

    None

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

20. pCMV-PE6c

    Plasmid

    #207853

    Purpose

    Mammalian expression of PE6c prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6c
    

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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