168,838 results

1. piggyBac CMV-GFP-FRT

   Plasmid

   #92078

   Purpose

   production of transgenic animal and recombination using flipase

   Depositor

   Jae Yong Han

   Article

   Lee et al FASEB J. 2016 Feb;30(2):555-63. doi: 10.

   Insert

   CMV-eGFP and TK-Neo between FRT3 and FRT
   

   Use

   Piggybac

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5 FLAG wildtype, catalytic active lipin 1

   Plasmid

   #32005

   Depositor

   David Sabatini

   Article

   Peterson et al Cell. 2011 Aug 5;146(3):408-20.

   Insert

   lipin1 (Lpin1 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV, SP6

   Available Since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+) EGFP-Rac1(WT)

   Plasmid

   #224278

   Purpose

   Cell transfection and expression of Rac1

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   Rac1 (RAC1 Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Construct 32 - NFKBRp_mKate2_2xNLS_p2a-puroR

   Plasmid

   #82024

   Purpose

   Lentiviral construct for building stable cell lines expressing mKate2 in the presence of TNFa via puromycin selection

   Depositor

   Timothy Lu

   Article

   Perli et al Science. 2016 Aug 18. pii: aag0511.

   Insert

   mKate2-2xNLS
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-mt4xi-GCaMP6f

   Plasmid

   #192075

   Purpose

   Fluorescent reporter for mitochondrial calcium

   Depositor

   Timothy Ryan

   Insert

   4 repeats of improved COX8 signal peptide plus GCaMP6f
   

   Expression

   Mammalian

   Available Since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. p5E-4xnrUAS (L4-R1)

   Plasmid

   #61372

   Purpose

   5' Gateway Entry Clone Containing 4xnrUAS

   Depositor

   Marnie Halpern

   Article

   Akitake et al Dev Biol. 2011 Apr 15;352(2):191-201. do

   Insert

   4xnrUAS
   

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. RKS-Fzd7 subtype NGS Wnt

   Plasmid

   #159626

   Purpose

   Express Fzd7 subtype NGS Wnt in mammalian cells

   Depositor

   Chris Garcia

   Article

   Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

   Insert

   Fzd7 subtype NGS Wnt
   

   Use

   Lentiviral

   Tags

   His tag

   Expression

   Mammalian

   Available Since

   Sept. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. p5xUAS-Flag-NPM1c

   Plasmid

   #131823

   Purpose

   4-hydroxytamoxifen inducible vector expressing Flag-NPM1c (cytoplasmic mutant, human) in mammalian cells through transduction

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   NPM1 (NPM1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1+/-FLI1-C‐(K)‐DYK

   Plasmid

   #129497

   Purpose

   Mammalian expression of human FLI1

   Depositor

   Marjorie Brand

   Article

   Palii et al Cell Stem Cell. 2019 May 2;24(5):812-820

   Insert

   FLI1 (FLI1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. mito-gTEMP_pcDNA3

    Plasmid

    #109117

    Purpose

    Mitochondria targetting gTEMP

    Depositor

    Takeharu Nagai

    Article

    Nakano et al PLoS One. 2017 Feb 17;12(2):e0172344. do

    Insert

    mito-gTEMP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. STARR-seq luciferase validation vector_ORI_CMV

    Plasmid

    #99312

    Purpose

    Luciferase validation vector with CMV enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Insert

    CMV enhancer
    

    Use

    Luciferase; Starr-seq luciferase validation vector

    Expression

    Mammalian

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRISPRi-D2

    Plasmid

    #182927

    Purpose

    inducible CRISPRi plasmid with gRNA targeting to psbD gene in Synechocystis 6803

    Depositor

    Himadri Pakrasi

    Article

    Liu et al ACS Synth Biol. 2020 Jun 19;9(6):1441-14

    Insert

    ddcpf1
    

    Use

    CRISPR

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. p2CT-His-MBP-Lba_Cas13a_WT

    Plasmid

    #91867

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    LbaCas13a (LbaC2c2) WT
    

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. tdTomato-Golgi-7

    Plasmid

    #58100

    Purpose

    Localization: Golgi, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Article

    Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

    Insert

    Golgi (B4GALT1 Human)
    

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    aa 1-82 of NM_001497.3

    Promoter

    CMV

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pCMV-EGFP-p50

    Plasmid

    #107977

    Purpose

    Expresssion of NF-kB p50

    Depositor

    Georg Weber

    Insert

    NF-kB p50 (Nfkb1 Mouse)
    

    Tags

    N/A

    Expression

    Mammalian

    Mutation

    Amino acids VQACI added to C-terminus

    Promoter

    CMV

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTomatoSlicer

    Plasmid

    #221085

    Purpose

    Dual-color splicing reporter expressing eGFP and tdTomato from the CMV early enhancer/chicken ß-actin (CAGGS) promoter.

    Depositor

    Chaolin Zhang

    Article

    Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDAS12125_PEAR-GFP

    Plasmid

    #177178

    Purpose

    PEAR-GFP target plasmid with split EGFP and disrupted 5' splice site

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    EGFP split with an intron between amino acids 95-96
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    disrupted 5' splice site

    Promoter

    CMV

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL3-Basic-Pax8

    Plasmid

    #11994

    Depositor

    Steven Johnson

    Insert

    PAX8 (PAX8 Human)
    

    Expression

    Mammalian

    Mutation

    The following construct contains the PAX8 promote…

    Available Since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-EF1a-FLEX-TVA-mCherry

    Plasmid

    #38044

    Depositor

    Naoshige Uchida

    Article

    Watabe-Uchida et al Neuron. 2012 Jun 7;74(5):858-73.

    Insert

    Avian sarcoma and leukemia virus receptor 950 (CD320 Synthetic, quail)
    

    Use

    AAV and Cre/Lox

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. LgBiT-PIK3CA Fusion Vector

    Plasmid

    #237092

    Purpose

    Express LgBiT-PIK3CA Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    PIK3CA (PIK3CA Human)
    

    Tags

    LgBiT

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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