168,515 results

1. Frt-hspB5

   Plasmid

   #63096

   Purpose

   Expression of HSPB5 (small HSP) in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb5 (CRYAB Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. DL016:CMV::eGFP-iPAK4

   Plasmid

   #177881

   Purpose

   eGFP-IPAK4 fusion with CMV promoter, cloned into a lentivirus backbone

   Depositor

   Adam Cohen

   Article

   Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   iPAK4
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLminP_Luc2P_RE50

   Plasmid

   #90389

   Purpose

   PPARg/RARa - PPARg gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Promoter

   ACO1

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDule-3-nitroTyrosine (5B)

   Plasmid

   #85498

   Purpose

   Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.

   Insert

   3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32H H70C D158S I159A L162R

   Promoter

   lpp (constitutive)

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST-CMV mCherry-GFP-LC3B G120A

   Plasmid

   #123235

   Purpose

   Expresses mCherry-EGFP-LC3B G120A in mammalian cells. Negative control for tandem reporter. High expression driven by CMV promoter.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Mutation

   Glycine 120 to Alanine

   Promoter

   CMV

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBAD-pHlorina

   Plasmid

   #182763

   Purpose

   pH-sensitive RFP pHlorina for detection of efferocytosis

   Depositor

   Robert Campbell

   Article

   Raymond et al Science. 2022 Mar 11;375(6585):1182-1187

   Insert

   pHlorina
   

   Expression

   Bacterial

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIRES-hrGFP II-TET1

   Plasmid

   #83568

   Purpose

   Expresses TET1 in mammalian cells

   Depositor

   Jean-Pierre Issa

   Article

   Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

   Insert

   Tet Methylcytosine Dioxygenase 1 (TET1 Human)
   

   Tags

   3X FLAG tag and hr GFP II

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV5-AML1B

   Plasmid

   #12426

   Purpose

   pCMV5-AML1B is the original plasmid used in Meyers et al 1995. However, please note that the insert sequence has now been identified as AML1C.

   Depositor

   Scott Hiebert

   Article

   Meyers et al Mol Cell Biol. 1995 Apr . 15(4):1974-82.

   Insert

   AML1B (RUNX1 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. Str-KDEL_ManII-SBP-EGFP

   Plasmid

   #65252

   Purpose

   synchronize trafficking of ManII from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Streptavidin-KDEL and truncated Mannosidase II
   

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 116 of ManII

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA4/TO/Strep-HA-ZAK beta

    Plasmid

    #141195

    Purpose

    Expresses Strep-HA-ZAK beta in mammalian cells, can be used to make inducible cell line

    Depositor

    Simon Bekker-Jensen

    Article

    Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

    Insert

    MAP3K20 isoform 2 (MAP3K20 Human)
    

    Tags

    Strep, HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVNP3.0-PEmax

    Plasmid

    #206883

    Purpose

    Expresses FLAG-tagged PEmax fused to Gag through a linker sequence

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

    Insert

    PEmax
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Promoter

    CMV

    Available Since

    Oct. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5FRT-EF-Pdgfrbeta-EGFPN

    Plasmid

    #66790

    Purpose

    Expression of murine PDGFRbeta tagged with GFP under the control of EF1a promoter

    Depositor

    Lotte Pedersen

    Article

    Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

    Insert

    PDGFRbeta (Pdgfrb Mouse)
    

    Tags

    C-term EGFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS-membrane-ceruleanFP

    Plasmid

    #53749

    Purpose

    Contains membrane localized cerulean fluorescent protein in pCS expression vector.

    Depositor

    Sean Megason

    Article

    Megason et al Methods Mol Biol. 2009;546:317-32. doi:

    Insert

    2x membrane linker
    

    Tags

    Cerulean

    Expression

    Mammalian

    Promoter

    simian CMV IE94

    Available Since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. tetO-AKT1

    Plasmid

    #170682

    Purpose

    doxycycline-inducible overexpression of AKT1

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet-ON

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 HA-BACH1

    Plasmid

    #232269

    Purpose

    Expression of N-terminal tagged HA-BACH1 (H. sapiens) in human cell lines.

    Depositor

    Michele Pagano

    Article

    Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

    Insert

    BACH1 (BACH1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. neurod1:cre; cmlc2:EGFP

    Plasmid

    #173480

    Purpose

    cre is driven by -5kb neurod1 promoter. The vector contain a cmlc2:EGFP maker

    Depositor

    John Rawls

    Article

    Ye et al Cell Host Microbe. 2021 Feb 10;29(2):179

    Insert

    Cre
    

    Use

    Zebrafish expression

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. DYRK1A

    Plasmid

    #38913

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    DYRK1A (DYRK1A Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1-CCND1

    Plasmid

    #172632

    Purpose

    Expresses untagged cyclin D1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSicoR p53

    Plasmid

    #12090

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF p53 shRNA expression.

    Depositor

    Tyler Jacks

    Article

    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

    Insert

    p53 shRNA (Trp53 Mouse)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Mutation

    EGFP is expressed from this plasmid as a marker, …

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO.1 Rheb1 shRNA #1

    Plasmid

    #26625

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    Rheb1 shRNA (RHEB Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only