168,838 results

1. mCh-alpha-tubulin

   Plasmid

   #49149

   Purpose

   expression of alpha-tubulin fused to mCherry. Used for visualization of microtubules.

   Depositor

   Gia Voeltz

   Article

   Friedman et al J Cell Biol. 2010 Aug 9;190(3):363-75. d

   Insert

   alpha tubulin (TUBA1B Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET 28 full-length human PARP1

   Plasmid

   #192635

   Purpose

   Expresses human PARP1 with an N-terminal His-tag

   Depositor

   John Pascal

   Article

   Langelier et al J Biol Chem. 2008 Feb 15;283(7):4105-14.

   Insert

   Human PARP1 (PARP1 Human)
   

   Tags

   histidine-tag

   Expression

   Bacterial

   Mutation

   Polymorphism A762

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. anxA2-GFP

   Plasmid

   #107196

   Purpose

   Expresses human annexin A2-GFP fusion protein for live cell imaging

   Depositor

   Volker Gerke, Ursula Rescher

   Article

   Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

   Insert

   annexin A2 (ANXA2 Human)
   

   Tags

   Green Fluorescent Protein

   Expression

   Mammalian

   Mutation

   Ala residue 65 in human sequence replaced by Glu …

   Promoter

   CMV

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDF0112 cmv-cluciferase-polya-ef1a-g-luciferase-polya

   Plasmid

   #186980

   Purpose

   mammalian expression of Cluc-Gluc reporter

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

   Insert

   Cluc, Gluc
   

   Use

   Luciferase

   Available Since

   Aug. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. 0_T7-mScarlet3

   Plasmid

   #225927

   Purpose

   In vitro synthesis of mRNA for fluorescent protein mScarlet3 (control)

   Depositor

   Michalis Averof

   Article

   Karapidaki et al bioRxiv 2024.11.12.623055

   Insert

   mScarlet3
   

   Use

   In vitro transcription

   Promoter

   T7

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pS148∙CsR

   Plasmid

   #197858

   Purpose

   Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

   Depositor

   Susanne Häußler

   Article

   Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

   Insert

   gRNA scaffold - Ap/Cb resistance cassette - incP oriT
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   PEM7

   Available Since

   Oct. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. EGFP-E-Syt1

   Plasmid

   #66830

   Purpose

   Mammalian Expression, Bacterial Expression

   Depositor

   Pietro De Camilli

   Article

   Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

   Insert

   E-Syt1 (ESYT1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PB CAG-sfGFP

   Plasmid

   #133568

   Purpose

   CAG sfGFP piggyBac transposon

   Depositor

   Mario Capecchi

   Insert

   sfGFP
   

   Expression

   Mammalian

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV- δLight

   Plasmid

   #201224

   Purpose

   Express deltaLight biosensor in mammalian cells

   Depositor

   Lin Tian

   Article

   Dong et al Nat Neurosci. 2024 Jul 15. doi: 10.1038/

   Insert

   δLight1.3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. BASIC_SEVA_67_pKD46.10

    Plasmid

    #178942

    Purpose

    BASIC DNA assembly vector

    Depositor

    Paul Freemont

    Article

    Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

    Insert

    mScarlet counter-selection cassette
    

    Use

    Synthetic Biology

    Available Since

    March 1, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pENTR/D_creERt2

    Plasmid

    #27321

    Depositor

    Leonard Zon

    Article

    Mosimann et al Development. 2011 Jan . 138(1):169-77.

    Insert

    creERt2
    

    Use

    Multisite gateway middle entry vector

    Available Since

    Jan. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUCmini-iCAP-AAV9.452sub.LUNG1

    Plasmid

    #184592

    Purpose

    non-standard AAV2 rep-AAV9.452sub.LUNG1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system for mouse lung transduction after intravenous injection

    Depositor

    Viviana Gradinaru

    Article

    Goertsen et al Mol Ther Methods Clin Dev. 2022 Aug 1;26

    Insert

    Synthetic construct isolate AAV9.452sub.LUNG1 VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    7 amino acid substitution between VP1 452 and VP1…

    Promoter

    p41

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDY0209 ACTB Nicking guide +48

    Plasmid

    #179109

    Purpose

    ACTB N-term Nicking guide 1 +48 guide

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    ACTB N-term Nicking guide 1 +48 guide
    

    Use

    CRISPR

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. eSrtA(2A-9) pET29b

    Plasmid

    #75145

    Purpose

    reprogrammed sortase A variant that recognizes alternative substrate LAXTG with high activity and specificity

    Depositor

    David Liu

    Article

    Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

    Insert

    reprogrammed sortase A variant
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    S102C A104H E105D K138P K152I N160K K162H T164N K…

    Promoter

    T7

    Available Since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CL20_mEGFP_NUP98_HOXD13

    Plasmid

    #205871

    Purpose

    Express mEGFP-tagged fusion protein, NUP98_HOXD13 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    NUP98_HOXD13 (NUP98, HOXD13 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSH824-ssr

    Plasmid

    #198027

    Purpose

    Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    T0 terminator - Apr resistance cassette
    

    Expression

    Bacterial

    Promoter

    AprR promoter

    Available Since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pZT-AAVS1-R1

    Plasmid

    #52638

    Purpose

    TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

    Depositor

    Mahendra Rao, Jizhong Zou

    Article

    Luo et al Stem Cells Transl Med. 2014 May 15. pii:

    Insert

    Right AAVS1 TALEN
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pZT-AAVS1-L1

    Plasmid

    #52637

    Purpose

    TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

    Depositor

    Mahendra Rao, Jizhong Zou

    Article

    Luo et al Stem Cells Transl Med. 2014 May 15. pii:

    Insert

    Left AAVS1 TALEN
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRK5_mEGFP-NUP98-DDX10-KS

    Plasmid

    #237644

    Purpose

    For overexpression of mEGFP-NUP98-DDX10-KS

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    NUP98-DDX10-KS (NUP98, DDX10 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-hSyn-PROGERIN

    Plasmid

    #140189

    Purpose

    Overexpression of Progerin with a GFP fusion protein, under human synapsin promotor

    Depositor

    Lorenz Studer

    Article

    Miller et al Cell Stem Cell. 2013 Dec 5;13(6):691-705

    Insert

    Progerin (LMNA Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge