168,515 results

1. pWPI_SPbFGF

   Plasmid

   #25812

   Depositor

   Jozsef Kiss, Patrick Salmon

   Article

   Dayer et al Brain. 2007 Nov . 130(Pt 11):2962-76.

   Insert

   pWPI_SPbFGF (FGF2 Human)
   

   Use

   Lentiviral

   Mutation

   Ig signal peptide in N-terminal of human FGF2

   Available Since

   Jan. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHBV-S1-Luc

   Plasmid

   #71416

   Purpose

   To see S1 promoter activity of HBV for luciferase assay

   Depositor

   Wang-Shick Ryu

   Article

   Ko et al J Virol. 2014 Dec;88(23):13689-98. doi:

   Insert

   HBV S1 promoter driving Luciferase expression
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   HBV S1

   Available Since

   Dec. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHBV-S2-Luc

   Plasmid

   #71417

   Purpose

   To see HBV S2 promoter activity for luciferase assay

   Depositor

   Wang-Shick Ryu

   Article

   Ko et al J Virol. 2014 Dec;88(23):13689-98. doi:

   Insert

   HBV S2 promoter driving luciferase expression
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   HBV S2

   Available Since

   Dec. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBJ3

   Plasmid

   #167132

   Purpose

   A kanamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoter

   Depositor

   Akira Mine

   Article

   Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

   Insert

   luxCDABE
   

   Use

   Luciferase

   Expression

   Bacterial

   Promoter

   Frr promoter

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. wg186Fluc

   Plasmid

   #177436

   Purpose

   In vitro synthesis of an mRNA encoding Firefly luciferase with A[62] tail.

   Depositor

   Wendy Gilbert

   Article

   Rojas-Duran et al RNA. 2012 Dec;18(12):2299-305. doi: 10.1

   Insert

   Firefly luciferase
   

   Use

   Luciferase; For in vitro transcription

   Tags

   None

   Promoter

   T7

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSSa26

   Plasmid

   #186784

   Purpose

   Expression of reverse transactivator (CgrtTA)

   Depositor

   Dominique Sanglard

   Article

   Schrevens et al FEMS Yeast Res. 2022 Sep 24;22(1):foac04

   Insert

   CgrtTA
   

   Expression

   Yeast

   Promoter

   ScPGK1p

   Available Since

   July 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCG-TPMV Hd32-IL-13

   Plasmid

   #163869

   Purpose

   Expresses TPMV H glycoprotein bearing IL-13 in mammalian cells

   Depositor

   Jakob Reiser

   Article

   Argaw et al Mol Ther Methods Clin Dev. 2021 Apr 24;2

   Insert

   TPMV H glycoprotein/IL-13
   

   Tags

   Human IL-13

   Expression

   Mammalian

   Mutation

   Amino acids 2 to 33 at N terminus deleted

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. HRH3-Tango

   Plasmid

   #66402

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   HRH3 (HRH3 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

9. MSCV-N 16E6

   Plasmid

   #37875

   Depositor

   Karl Munger

   Article

   Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

   Insert

   HPV 16E6 (E6 HPV)
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Promoter

   PKG

   Available Since

   July 25, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pU1_000

    Plasmid

    #186773

    Purpose

    * Engineered U1 snRNA plasmid for insertion of guide sequences.

    Depositor

    Eugene Yeo

    Article

    Hatch et al Methods. 2022 Jun 25;205:140-148. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial and Mammalian

    Promoter

    U1 Promoter

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. TetO-FUW-PLZF

    Plasmid

    #61543

    Purpose

    Expresses PLZF in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    PLZF (ZBTB16 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    I380T and G580D

    Available Since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. HRH1-Tango

    Plasmid

    #66400

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    HRH1 (HRH1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pUb-G76V-GFP-IRES-DsRed

    Plasmid

    #225503

    Purpose

    Expression of Ub-G76V-GFP along dsRED from one mRNA

    Depositor

    David Rubinsztein

    Article

    Yu et al Hum Mol Genet. 2024 May 22:ddae085. doi:

    Insert

    Ub-G76V-GFP
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Ubiquitin fused to N-terminus of GFP. Glycine 76 …

    Promoter

    CMV promoter

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFEndoNA

    Plasmid

    #174724

    Purpose

    Expresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.

    Depositor

    Jukka Finne

    Article

    Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149

    Insert

    GFP-endoNA fusion protein
    

    Tags

    Histag

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLRD01

    Plasmid

    #208257

    Purpose

    Production of terpentetriene in E. coli MEV20

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Inserts

    tHMGR
    cyc1
    cyc2
    

    Expression

    Bacterial

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. B52_puro_empty_gRNA

    Plasmid

    #197557

    Purpose

    sites for cloning two gRNA with puromycin cassette

    Depositor

    Darius Bägli

    Type

    Empty backbone

    Use

    Empty grna backbone with puromycin resistance gene

    Expression

    Mammalian

    Promoter

    U6 for gRNA, CMV for puromycin resistance.

    Available Since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF1843

    Plasmid

    #141877

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ALX1 (ALX1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMM764

    Plasmid

    #68901

    Purpose

    IPTG-inducible CRISPRi vector targeting nonsense sequence (NS), constiutive NanoLuc, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    NanoLuc
    

    Mutation

    L51P

    Available Since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. p-mCherry2-sgRNA (empty)

    Plasmid

    #198330

    Purpose

    Customisable sgRNA sequence with optimised sgRNA scaffold for expression under U6 promoter, with mCherry2 reporter

    Depositor

    Sarah McClelland

    Article

    Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

    Insert

    U6-sgRNA(F+E) empty
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKV2-hU6-BbsI-PGK-Puro-TagBFP-WPRE

    Plasmid

    #137994

    Purpose

    Backbone for generation of ClonMapper gRNA barcode library for use in 10X feature barcoding

    Depositor

    Amy Brock

    Article

    Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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