168,808 results

1. pBR322-TIMER

   Plasmid

   #103056

   Purpose

   medium copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)

   Depositor

   Dirk Bumann

   Article

   Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1

   Insert

   TIMER-bac
   

   Expression

   Bacterial

   Mutation

   mutations: S4T and S179T

   Promoter

   constitutive ybaJp promoter

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. VEGFR2-mCh

   Plasmid

   #108854

   Purpose

   Encodes for human VEGFR2 fluorescently labeled with mCherry on the C-Terminus via a 3 amino acid (GGS) flexible linker

   Depositor

   Kalina Hristova

   Article

   Sarabipour et al Elife. 2016 Apr 7;5:e13876. doi: 10.7554

   Insert

   KDR (KDR Human)
   

   Tags

   Labeled with mCherry on the C-Terminus via a 3 am…

   Expression

   Mammalian

   Available Since

   May 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Xla.Tubb2:hArr-TEV;he1.1:eYFP

   Plasmid

   #218859

   Purpose

   Xla.Tubb2 promoter driving expression of human Arrestin-TEV protease fusion protein for use in trans-Tango.

   Depositor

   Marnie Halpern

   Article

   Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

   Insert

   hArr
   

   Tags

   human Arrestin-Tobbaco Etch Virus protease fusion…

   Expression

   Bacterial

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-WT-STAT1

   Plasmid

   #71454

   Purpose

   Lentiviral expression of WT-STAT1

   Depositor

   George Stark

   Article

   Cheon et al Proc Natl Acad Sci U S A. 2009 Jun 9;106

   Insert

   STAT1 (STAT1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Promoter

   CMV

   Available Since

   Dec. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. bact2-mKate2-P2A-TurboID-dVHH[GFP]

   Plasmid

   #163857

   Purpose

   Zebrafish ubiquitous specific expression of mKate2-P2A-TurboID-dVHH[GFP]. Parton lab clone JBH

   Depositor

   Rob Parton

   Article

   Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

   Insert

   TurboID-VHH[EGFP]
   

   Use

   Zebrafish expression

   Tags

   mKate2-P2A-TurboID

   Available Since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. RAD21 C-tag CRISPR

   Plasmid

   #140540

   Purpose

   RAD21 tagging CRISPR

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   RAD21 (RAD21 Human)
   

   Use

   CRISPR

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-N1 human cofilin WT

   Plasmid

   #50859

   Purpose

   expresses human cofilin fused to EGFP in mammalian cells

   Depositor

   James Bamburg

   Article

   Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

   Insert

   cofilin 1 (CFL1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-GFP-IMP2-2

   Plasmid

   #42175

   Depositor

   Alexandra Kiemer

   Article

   Kessler et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   Insulin-like growth factor 2 mRNA binding protein 2-2 (IGF2BP2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   June 14, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pEGFP-C1-FLAG-Ku70

   Plasmid

   #46957

   Purpose

   Plasmid for cloning and epression of GFP-FLAG tagged human Ku70 (N-terminal tag). Confers resistance to G418.

   Depositor

   Steve Jackson

   Article

   Britton et al J Cell Biol. 2013 Jul 29.

   Insert

   Ku70 (XRCC6 Human)
   

   Tags

   GFP-FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDestTol2pA2-9xGRE

    Plasmid

    #74736

    Purpose

    GRE-dependent EGFP reporter construct flanked by the minimal Tol2 transposon elements

    Depositor

    Luisa Dalla Valle

    Article

    Benato et al Mol Cell Endocrinol. 2014 Jul 5;392(1-2)

    Insert

    EGFP
    

    Use

    Zebrafish expression

    Promoter

    nine GC-responsive elements (9xGRE) from the rat …

    Available Since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMK262 (RAD21-mAC Neo)

    Plasmid

    #140538

    Purpose

    RAD21 tagging with mAID-Clover

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    RAD21 (RAD21 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYZ463

    Plasmid

    #187971

    Purpose

    TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

    Insert

    Cas9 (codon optimized for human)
    

    Use

    Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPL5071_TEF1*-Cre_URA3

    Plasmid

    #60930

    Purpose

    Constitutive expression vector for Cre-recombinase containing URA3 selection marker.

    Depositor

    Robert Piper

    Article

    MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

    Insert

    Cre
    

    Tags

    Driven from a mutant TEF1 promoter with 0.16x act…

    Expression

    Yeast

    Promoter

    mutant TEF1

    Available Since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-mScarlet3-CAAX-IRES-Puro

    Plasmid

    #222639

    Purpose

    Live-cell plasma membrane marker

    Depositor

    Arnold Hayer

    Article

    Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

    Insert

    mScarlet3-CAAX
    

    Use

    Lentiviral

    Tags

    mScarlet3

    Promoter

    EF1a

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. p10xUAS-luc2

    Plasmid

    #173006

    Purpose

    firefly luciferase reporter vector with 10x clustered UAS binding sites

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    10xUAS-luc2
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. BL21 ΔrecBCD

    Bacterial Strain

    #176581

    Purpose

    E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression.

    Depositor

    Chase Beisel, Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    None

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

17. Xla.Tubb2:hGCGR-TEVcs-QF;he1.1:CFP

    Plasmid

    #218857

    Purpose

    Xla.Tubb2 promoter driving expression of human glucagon receptor, TEV protease recognition site, QF fusion protein for use in trans-Tango.

    Depositor

    Marnie Halpern

    Article

    Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

    Insert

    hGCGR (GCGR Human)
    

    Tags

    QF fusion protein

    Expression

    Bacterial

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET246-pUC57 12xMS2V6

    Plasmid

    #104390

    Purpose

    MS2V6 cloning

    Depositor

    Robert Singer, Evelina Tutucci

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    12xMS2V6
    

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. GPRC5A-Tango

    Plasmid

    #66382

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPRC5A (GPRC5A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO.3G_X7

    Plasmid

    #171213

    Purpose

    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses eGFP for selection. Please see cloning information for suggested restriction sites

    Depositor

    Jenny Wang

    Insert

    N/A; empty backbone
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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