222,579 results

1. pCIS.MTRIA-C3a

   Plasmid

   #184601

   Purpose

   For expression of a fluorescent sensor for C3a complement in mammalian cells

   Depositor

   Hiroshi Hibino, Daisuke Ino

   Article

   Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

   Insert

   MTRIA-C3a
   

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. YIplac211-NHX1-iGFP

   Plasmid

   #177108

   Purpose

   Expresses Nhx1-iGFP in yeast cells

   Depositor

   Benjamin Glick

   Article

   Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

   Insert

   NHX1 (NHX1 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.TBG.PI.eGFP.WPRE.bGH (AAV8)

   Viral Prep

   #105535-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV.TBG.PI.eGFP.WPRE.bGH (#105535). In addition to the viral particles, you will also receive purified pAAV.TBG.PI.eGFP.WPRE.bGH plasmid DNA. TBG-driven eGFP expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   TBG

   Tags

   EGFP

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

4. pAAV-FLEX-tdTomato (AAV Retrograde)

   Viral Prep

   #28306-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. Cre-dependent (FLEX) tdTomato expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Edward Boyden

   Promoter

   CAG

   Tags

   tdTomato (Cre-dependent)

   Available Since

   Dec. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

5. pCMV-VSV-G

   Plasmid

   #8454

   Purpose

   Envelope protein for producing lentiviral and MuLV retroviral particles. Use in conjunction with a packaging vector such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral).

   Depositor

   Bob Weinberg

   Article

   Stewart et al RNA 2003 Apr;9(4):493-501.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   June 20, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-VSV-G

   Plasmid

   #8454

   Purpose

   Envelope protein for producing lentiviral and MuLV retroviral particles. Use in conjunction with a packaging vector such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral).

   Depositor

   Bob Weinberg

   Article

   Stewart et al RNA 2003 Apr;9(4):493-501.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   June 20, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX-TetOne-Puro-GFP

   Plasmid

   #171123

   Purpose

   Doxycycline inducible expression of GFP

   Depositor

   Jason Sheltzer

   Article

   Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

   Insert

   EGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE3GS

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. TRUPATH Triple Gai2

   Plasmid

   #196049

   Purpose

   Encodes a G alpha subunit (GNAl2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphai2-RLuc8 (GNAI2 Human)
   GGamma8-GFP2 (GNG8 Human)
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. TRUPATH Triple Gai3

   Plasmid

   #196050

   Purpose

   Encodes a G alpha subunit (GNAl3) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphai3-RLuc8 (GNAI3 Human)
   GGamma9-GFP2
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV Retrograde)

    Viral Prep

    #44362-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-DIO-hM4D(Gi)-mCherry (#44362). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM4D(Gi)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Article

    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

    Promoter

    Syn

    Tags

    mCherry (Cre-dependent)

    Available Since

    June 21, 2017

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-mDlx-ChR2-mCherry-Fishell-3 (AAV9)

    Viral Prep

    #83898-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-mDlx-ChR2-mCherry-Fishell-3 (#83898). In addition to the viral particles, you will also receive purified pAAV-mDlx-ChR2-mCherry-Fishell-3 plasmid DNA. mDlx-driven expression of ChR2, fused to mCherry. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Gordon Fishell

    Article

    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

    Promoter

    mDlx

    Tags

    mCherry

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

12. pAAV-hSyn-DIO-mCherry (AAV9)

    Viral Prep

    #50459-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hSyn-DIO-mCherry (#50459). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-mCherry plasmid DNA. hSyn-driven, Cre-dependent mCherry-expression control. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Promoter

    Syn

    Tags

    mCherry (Cre-dependent)

    Available Since

    April 25, 2019

    Availability

    Academic Institutions and Nonprofits only

13. pLKO.1 - TRC cloning vector

    Plasmid

    #10878

    Purpose

    3rd generation transfer plasmid

    Depositor

    David Root

    Article

    Moffat et al Cell. 2006 Mar 24. 124(6):1283-98.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1 - TRC cloning vector

    Plasmid

    #10878

    Purpose

    3rd generation transfer plasmid

    Depositor

    David Root

    Article

    Moffat et al Cell. 2006 Mar 24. 124(6):1283-98.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. Tet-pLKO-puro

    Plasmid

    #21915

    Purpose

    3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.

    Depositor

    Dmitri Wiederschain

    Article

    Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Aug. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. Tet-pLKO-puro

    Plasmid

    #21915

    Purpose

    3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.

    Depositor

    Dmitri Wiederschain

    Article

    Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Aug. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-FLEX-EGFPL10a (AAV5)

    Viral Prep

    #98747-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-FLEX-EGFPL10a (#98747). In addition to the viral particles, you will also receive purified pAAV-FLEX-EGFPL10a plasmid DNA. Cre-dependent EGFPL10a (EGFP-tagged ribosomal protein) expression for translating ribosome affinity purification (TRAP). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Nathaniel Heintz, Alexander Nectow, Eric Schmidt

    Article

    Nectow et al Cell Rep. 2017 Apr 18;19(3):655-667. doi

    Promoter

    EF1a

    Tags

    EGFP (Cre-dependent)

    Available Since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

18. pT4-CMV-GFP

    Plasmid

    #117046

    Purpose

    Optimized Sleeping Beauty Transposon Vector for GFP expression by a CMV promoter

    Depositor

    Wolfgang Uckert

    Insert

    GFP
    

    Use

    Transposon

    Promoter

    CMV

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-PEmax-P2A-BSD

    Plasmid

    #174821

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-BSD marker

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax-P2A-BSD
    

    Tags

    P2A-BSD, SV40 bpNLS, and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV9)

    Viral Prep

    #44362-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hSyn-DIO-hM4D(Gi)-mCherry (#44362). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM4D(Gi)-mCherry plasmid DNA. hSyn-driven, Cre-dependent, hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Article

    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

    Promoter

    Syn

    Tags

    mCherry (Cre-dependent)

    Available Since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only