168,503 results

1. DL015:CMV::iPAK4

   Plasmid

   #177880

   Purpose

   ibox-PAK4cat (iPAK4) fusion with CMV promoter, cloned into a lentivirus backbone

   Depositor

   Adam Cohen

   Article

   Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   iPAK4
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCVL.SFFV.d14GFP.EF1a.HA.NLS.Sce(opt).T2A.TagBFP

   Plasmid

   #32627

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   Donor + Sce BFP
   

   Use

   Lentiviral

   Available Since

   Nov. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDAS12222_U6-pegRNA-BFP

   Plasmid

   #177181

   Purpose

   pegRNA cloning plasmid with BFP marker

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. BirA-Myc_N_PAX6

   Plasmid

   #167721

   Purpose

   Expresses PAX6 in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   PAX6 (PAX6 Human)
   

   Tags

   Myc-BirA

   Expression

   Mammalian

   Mutation

   no mutations

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.2/NS1

   Plasmid

   #120824

   Purpose

   Ectopic expression of ZIKV protein

   Depositor

   Jan Rehwinkel

   Article

   Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

   Insert

   NS1 ZIKV
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-LiMETER-v2

   Plasmid

   #113934

   Purpose

   Also termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFP

   Depositor

   Yubin Zhou

   Article

   He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi

   Insert

   LOV2 and Stim1 polybasic domain (PB)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Frt-hspB5

   Plasmid

   #63096

   Purpose

   Expression of HSPB5 (small HSP) in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb5 (CRYAB Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. DL016:CMV::eGFP-iPAK4

   Plasmid

   #177881

   Purpose

   eGFP-IPAK4 fusion with CMV promoter, cloned into a lentivirus backbone

   Depositor

   Adam Cohen

   Article

   Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   iPAK4
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLminP_Luc2P_RE50

   Plasmid

   #90389

   Purpose

   PPARg/RARa - PPARg gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Promoter

   ACO1

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDule-3-nitroTyrosine (5B)

    Plasmid

    #85498

    Purpose

    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.

    Insert

    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32H H70C D158S I159A L162R

    Promoter

    lpp (constitutive)

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST-CMV mCherry-GFP-LC3B G120A

    Plasmid

    #123235

    Purpose

    Expresses mCherry-EGFP-LC3B G120A in mammalian cells. Negative control for tandem reporter. High expression driven by CMV promoter.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Glycine 120 to Alanine

    Promoter

    CMV

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBAD-pHlorina

    Plasmid

    #182763

    Purpose

    pH-sensitive RFP pHlorina for detection of efferocytosis

    Depositor

    Robert Campbell

    Article

    Raymond et al Science. 2022 Mar 11;375(6585):1182-1187

    Insert

    pHlorina
    

    Expression

    Bacterial

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIRES-hrGFP II-TET1

    Plasmid

    #83568

    Purpose

    Expresses TET1 in mammalian cells

    Depositor

    Jean-Pierre Issa

    Article

    Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

    Insert

    Tet Methylcytosine Dioxygenase 1 (TET1 Human)
    

    Tags

    3X FLAG tag and hr GFP II

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV5-AML1B

    Plasmid

    #12426

    Purpose

    pCMV5-AML1B is the original plasmid used in Meyers et al 1995. However, please note that the insert sequence has now been identified as AML1C.

    Depositor

    Scott Hiebert

    Article

    Meyers et al Mol Cell Biol. 1995 Apr . 15(4):1974-82.

    Insert

    AML1B (RUNX1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. Str-KDEL_ManII-SBP-EGFP

    Plasmid

    #65252

    Purpose

    synchronize trafficking of ManII from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin-KDEL and truncated Mannosidase II
    

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA4/TO/Strep-HA-ZAK beta

    Plasmid

    #141195

    Purpose

    Expresses Strep-HA-ZAK beta in mammalian cells, can be used to make inducible cell line

    Depositor

    Simon Bekker-Jensen

    Article

    Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

    Insert

    MAP3K20 isoform 2 (MAP3K20 Human)
    

    Tags

    Strep, HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVNP3.0-PEmax

    Plasmid

    #206883

    Purpose

    Expresses FLAG-tagged PEmax fused to Gag through a linker sequence

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

    Insert

    PEmax
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Promoter

    CMV

    Available Since

    Oct. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5FRT-EF-Pdgfrbeta-EGFPN

    Plasmid

    #66790

    Purpose

    Expression of murine PDGFRbeta tagged with GFP under the control of EF1a promoter

    Depositor

    Lotte Pedersen

    Article

    Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

    Insert

    PDGFRbeta (Pdgfrb Mouse)
    

    Tags

    C-term EGFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS-membrane-ceruleanFP

    Plasmid

    #53749

    Purpose

    Contains membrane localized cerulean fluorescent protein in pCS expression vector.

    Depositor

    Sean Megason

    Article

    Megason et al Methods Mol Biol. 2009;546:317-32. doi:

    Insert

    2x membrane linker
    

    Tags

    Cerulean

    Expression

    Mammalian

    Promoter

    simian CMV IE94

    Available Since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. tetO-AKT1

    Plasmid

    #170682

    Purpose

    doxycycline-inducible overexpression of AKT1

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet-ON

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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