Showing: 21 - 40 of 109 results
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Plasmid#161946PurposeContains Type IIS restriction site free elements for IBM staging vectors. Additional BsaI site to facilitate size selection. Staging plasmid for IBM on TetRDepositorInsertpSB1C3(BsmBI-,BsaI+)-BsaI-TetR(20-188)-BsaI
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
IBMc227
Plasmid#161948PurposeContains Type IIS restriction site free elements for expression vectors in IBM. Expression vector to generate ORF insertion library in IBM on TetRDepositorInsertpSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR(1-20)-BsaI-BsaI-TetR(188-207)-Ter
UseTagsExpressionBacterialMutationPromoterPBADAvailabilityAcademic Institutions and Nonprofits only -
IBMc259
Plasmid#161951PurposeContains elements for building reporter plasmid for IBM.TetR reporter plasmidDepositorInsertpSEVA471-PTet*-RiboJ10-B64-mScarlet-I-Ter
UseTagsExpressionBacterialMutationPromoterPTet*AvailabilityAcademic Institutions and Nonprofits only -
PSMC2_GFP_Cterm
Plasmid#221516PurposeFluorescently tagged Psmc2-GFPDepositorInsertPSMC2 (PSMC2 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Mc2r
Plasmid#48174Purposecontains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame elementDepositorInsert5" UTR of MC2R (MC2R Human)
UseLuciferaseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
MC2R-Tango
Plasmid#66428PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertMC2R (MC2R Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
SMC2 CRISPR
Plasmid#140649PurposeSMC2 tagging CRISPRDepositorInsertSMC2 (SMC2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
GFP-MuTMC2truncated
Plasmid#154909PurposeFor the expression and purification of the truncated TMC2 protein from Melopsittacus undulates. The protease cleavage site was replaced with the HRV 3C site.DepositorInsertGFP-MuTMC2truncated
UseTags8histidine-EGFPExpressionInsectMutationTruncations of the residues 1-179 and 691-716PromoterAvailabilityAcademic Institutions and Nonprofits only -
pRMC2-msfGFP
Plasmid#194913PurposeTetracycline inducible expression of msfGFP in StaphylococciDepositorInsertShine Dalgarno Sequence followed by monomeric superfolder GFP
UseTetracycline-inducible expression vector for stap…TagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pRMC2-sec:msfGFP
Plasmid#194914PurposeTetracycline inducible expression of msfGFP fused to Sec signal peptide in StaphylococciDepositorInsertShine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
UseTetracycline-inducible expression vector for stap…TagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pRMC2-tat:msfGFP
Plasmid#194915PurposeTetracycline inducible expression of msfGFP fused to Tat signal peptide in StaphylococciDepositorInsertShine Dalgarno sequence followed by Tat signal peptide fused to monomeric superfolder GFP
UseTetracycline-inducible expression vector for stap…TagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
MC2R-DuET
Plasmid#213336PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertMC2R (MC2R Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Mc2r-mod
Plasmid#48173Purposecontains Renilla Luciferase gene preceded by a mutated (ATG --> GTG) upstream open reading frame elementDepositorInsert5" UTR of MC2R (MC2R Human)
UseLuciferaseTagsExpressionMutationA/G MUTATION IN ATG OF UORFPromoterAvailabilityAcademic Institutions and Nonprofits only -
PGRMC2-CD4d3+4-bio
Plasmid#73112PurposeEXPRESs plasmid for human erythrocyte surface proteins encoding PGRMC2 with rat CD4d3+4-bioDepositorInsertPGRMC2 (PGRMC2 Human)
UseTagsbiotinylation peptide and ratCD4d3+4ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
SMC2 H5.1 gRNA
Plasmid#90892Purpose3rd generation lentiviral gRNA plasmid targeting human SMC2DepositorInsertSMC2 (Guide Designation H5.1)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
SMC2 H6.1 gRNA
Plasmid#90893Purpose3rd generation lentiviral gRNA plasmid targeting human SMC2DepositorInsertSMC2 (Guide Designation H6.1)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-PGRMC2
Plasmid#173468PurposeHuman PGRMC2 containing an N-terminal FLAG tagDepositorInsertPGRMC2 (PGRMC2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMC234r-ccdB/TPK2
Plasmid#176179Purposeyeast MoClo level-0 part plasmid type 234r containing toxic gene dropout cassette (ccdB and TPK2)DepositorInsertdropout cassette containing toxic genes ccdB and TPK2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
mc2 neo EF1a
Plasmid#206220PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
mc2 puro EF1a
Plasmid#206221PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 21 - 40 of 109 results