Addgene

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mc2 neo

Plasmid

#206214

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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mc2 puro

Plasmid

#206215

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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mc2 hygro

Plasmid

#206216

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
mc2 blast

Plasmid

#206217

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
mc2 mNeon

Plasmid

#206218

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
mc2 mCherry

Plasmid

#206219

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pRMC2

Plasmid

#68940

Purpose

tetracycline-inducible expression vector for Staphylococcus aureus

Depositor

Tim Foster

Article

Corrigan et al Plasmid. 2009 Mar;61(2):126-9. doi: 10.1

Type

Empty backbone

Use

Tet inducible e. coli/staphylococcus shuttle vect…

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pMC2m

Plasmid

#17970

Depositor

Sabine Ehrt

Article

Guo et al J Bacteriol. 2007 Jul . 189(13):4614-23.

Insert

tetR (B)

Use

Mycobacteria expression

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pMC2SG5

Plasmid

#31283

Depositor

Aziz Sancar

Article

Ye et al J Biol Chem. 2011 May 27. ():.

Insert

Cry2 (Cry2 Mouse)

Use

Tags

Flag, His, and Myc

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
IBMc233

Plasmid

#161939

Purpose

Carries Golden Gate substitution insert for IBM with M86 intein. PPhlF as C-lobe promoter

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB1A3-BbsI-M86N-T-cat-PPhlF-RiboJ-B32-M86C-SapI

Use

Tags

Expression

Bacterial

Mutation

Promoter

PPhlF

Availability

Academic Institutions and Nonprofits only

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IBMc226

Plasmid

#161944

Purpose

Contains Type IIS restriction site free elements for expressing protein to be split in IBM. TetR expression plasmid

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR-Ter

Use

Tags

Expression

Bacterial

Mutation

Promoter

PBAD

Availability

Academic Institutions and Nonprofits only

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IBMc228

Plasmid

#161946

Purpose

Contains Type IIS restriction site free elements for IBM staging vectors. Additional BsaI site to facilitate size selection. Staging plasmid for IBM on TetR

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB1C3(BsmBI-,BsaI+)-BsaI-TetR(20-188)-BsaI

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
IBMc227

Plasmid

#161948

Purpose

Contains Type IIS restriction site free elements for expression vectors in IBM. Expression vector to generate ORF insertion library in IBM on TetR

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR(1-20)-BsaI-BsaI-TetR(188-207)-Ter

Use

Tags

Expression

Bacterial

Mutation

Promoter

PBAD

Availability

Academic Institutions and Nonprofits only

Enlarge
IBMc259

Plasmid

#161951

Purpose

Contains elements for building reporter plasmid for IBM.TetR reporter plasmid

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSEVA471-PTet*-RiboJ10-B64-mScarlet-I-Ter

Use

Tags

Expression

Bacterial

Mutation

Promoter

PTet*

Availability

Academic Institutions and Nonprofits only

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PSMC2_GFP_Cterm

Plasmid

#221516

Purpose

Fluorescently tagged Psmc2-GFP

Depositor

Alessandro Ori

Insert

PSMC2 (PSMC2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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MC2R-Tango

Plasmid

#66428

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

MC2R (MC2R Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
psicheck-2-Mc2r

Plasmid

#48174

Purpose

contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of MC2R (MC2R Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
GFP-MuTMC2truncated

Plasmid

#154909

Purpose

For the expression and purification of the truncated TMC2 protein from Melopsittacus undulates. The protease cleavage site was replaced with the HRV 3C site.

Depositor

Motoyuki Hattori

Article

Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d

Insert

GFP-MuTMC2truncated

Use

Tags

8histidine-EGFP

Expression

Insect

Mutation

Truncations of the residues 1-179 and 691-716

Promoter

Availability

Academic Institutions and Nonprofits only

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pRMC2-msfGFP

Plasmid

#194913

Purpose

Tetracycline inducible expression of msfGFP in Staphylococci

Depositor

Rikke Meyer

Article

Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

Insert

Shine Dalgarno Sequence followed by monomeric superfolder GFP

Use

Tetracycline-inducible expression vector for stap…

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pRMC2-sec:msfGFP

Plasmid

#194914

Purpose

Tetracycline inducible expression of msfGFP fused to Sec signal peptide in Staphylococci

Depositor

Rikke Meyer

Article

Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

Insert

Shine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP

Use

Tetracycline-inducible expression vector for stap…

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge