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Showing: 21 - 40 of 16005 results

  11. CMVp-dsRed2-Triplex-28-gRNA3-28-gRNA4-28-gRNA5-28-gRNA6-28-pA

    Plasmid
    #55202
    Purpose
    Plasmid encoding multiplexed 4x gRNAs. This is a modified form of the original plasmid described in the paper (Construct 19). mKate2 was replaced with dsRed2 because of distribution issues.
    Depositor
    Timothy Lu
    Article
    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1
    Insert
    dsRed2
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    55202-plasmid-map-sequence-id-85589

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  15. PE-pegRNA(BG)-nsgRNA(BG)

    Plasmid
    #232727
    Purpose
    A dual-targeting vector that contains PE3 system guide RNAs against BFP (pegRNA(BG) and nsgRNA(BG)) with restriction enzyme sites for insertion of target site guide RNAS (pegRNAs(TS) and nsgRNA(TS)).
    Depositor
    David Brafman
    Article
    Frisch et al Mol Ther Nucleic Acids. 2023 Jul 17;33:4
    Insert
    pegRNA(BG), nsgRNA(BG), pegRNAs(TS), nsgRNA(TS)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232727-plasmid-map-sequence-id-462122

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  20. pJJF439_PU6_empty_sgRNAscaffold - sgRNA cloning backbone

    Plasmid
    #164266
    Purpose
    sgRNA cloning backbone for expression of sgRNA in C. elegans. Contains BbsI sites for insertion of spacer sequences. U6 promoter from W05B2.8 gene.
    Depositor
    Nikolaus Rajewsky
    Article
    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Worm
    Mutation
    Promoter
    U6 promoter from W05B2.8
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    164266-plasmid-map-sequence-id-325780

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Showing: 21 - 40 of 16005 results