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Plasmid#121742PurposepMVP R4-R3 entry plasmid, contains osTir1 for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertosTir1
UseSynthetic Biology; Pmvp gateway entry plasmidTags9x myc epitope tagExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
PLC-TIR-GFP
Plasmid#52736PurposeRetroviral vector expresses the lipid binding domain of PLCd1 fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable markerDepositorInsertToll/IL-1 Receptor adaptor protein (TIRAP Human)
UseRetroviralTagsGFP and PLCd1 Lipid bindingExpressionMammalianMutationDeleted amino acids 1-85PromoterAvailable sinceJune 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
SLP2a-TIR-GFP
Plasmid#52738PurposeRetroviral vector expresses the lipid binding domain of SLP2a fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable markerDepositorInsertToll/IL-1 Receptor adaptor protein (TIRAP Human)
UseRetroviralTagsC2A domain of Murine SLP2a and GFPExpressionMammalianMutationDeleted amino acids 1-85PromoterAvailable sinceJune 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOpen-NotIR (GC)
Plasmid#165507PurposeType II restriction enzyme that recognizes the double-stranded sequence GCGGCCGC and cleaves after C-1.DepositorInsertNotI (GC-rich recognition site)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRS306-OsTIR1F74A
Plasmid#198416PurposeExpresses OsTIR1F74A under the control of ADH1 promoter in budding yeastDepositorInsertOsTIR1F74A
UseTagsExpressionYeastMutationPromoterADH1 promoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiRCas9-CUG
Plasmid#104183PurposeLentiviral transfer vector that carries U6-driven sgRNA targeting CUG repeats using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)DepositorInsertU6-CUGsgRNA, EFS-PIN-dCas9
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
p285 TIR1_HA_mH11
Plasmid#164855Purposeconstitutive TIR1 expression vector for mouse H11 locusDepositorInsertOsTIR1
UseTagsHA tagExpressionMammalianMutationPromoterEF1-alphaAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-Ostir-neo
Plasmid#161973PurposeExpresses Ostir and G418 resistance gene in mammalian cellsDepositorInsertOstir-3xHA-p2a-neoR
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOsTIR1w/oGFP
Plasmid#102883PurposeExpresses OsTIR1 via the ADH1 promoter in yeast. The cassette is integrated in the HO locus. Assembly and characterization of the auxin-inducible degradation.DepositorInsertOsTIR1
UseTags3x mycExpressionYeastMutationPromoterADH1Available sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-TIR1-HA
Plasmid#172610PurposeLentiviral vector for the Tet-inducible expression of HA-tagged TIR1 and a constitutive puromycin resistance marker in mammalian cellsDepositorInsertTIR1
UseLentiviralTagsHAExpressionMammalianMutationPromoterTet/ON Minimal CMV promoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Punc-4c.TIR1
Plasmid#194053PurposeTIR1 expression vector under the unc-4c promoter expressed in the DA motor neurons in C. elegansDepositorInsertTIR1 (TIR1 Mustard Weed)
UseTagsExpressionWormMutationcodon-optimized for expression in C. elegans; Two…Promoterunc-4cAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOpen-PstIR
Plasmid#165526PurposeRestriction enzyme that recognizes the double-stranded sequence CTGCAG and cleaves after A-5.DepositorInsertPstI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
PB-Ostir-puro
Plasmid#161972PurposeExpresses Ostir and puromycin resistance gene in mammalian cellsDepositorInsertOstir-3xHA-p2a-puroR
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiRCas9-Lambda2
Plasmid#104184PurposeLentiviral transfer vector that carries U6-driven non-targeting sgRNA using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)DepositorInsertU6-sgRNA, EFS-PIN-dCas9
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
TIRAP-GFP
Plasmid#52739PurposeRetroviral vector expresses WT hTIRAPfused to GFP. Also contains IRES-hCD2 as selectable markerDepositorInsertToll/IL-1 Receptor adaptor protein (TIRAP Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTRE-BI-osTIR1
Plasmid#207840PurposeInducible TIR1 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cellsDepositorInsertsosTIR
mCherry
mAID_-VHH-GFP4
UseTags3X HA-Tag and weak NLSExpressionMammalianMutationAll K mutated to RPromoterTRE3G BI promoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR-TIR1
Plasmid#83875PurposeGateway donor vector for TIR1DepositorInsertTIR1 (TIR1 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGP8GF-AtTIR1
Plasmid#188427PurposeArabidopsis Nuclear Auxin Signaling Receptor TIR1DepositorInsertAuxin Signaling Receptor TIR1 (TIR1 Mustard Weed)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
p286 TIR1_HA_hH11
Plasmid#164853Purposeconstitutive TIR1 expression vector for human H11 locusDepositorInsertOsTIR1
UseTagsHA tagExpressionMammalianMutationPromoterEF1-alphaAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
TIRAP-TAP_IRES_GFP
Plasmid#52740PurposeRetroviral vector expresses hTIRAP-3xHA and a target site for the biotin ligase BirA upstream of IRES and GFPDepositorInsertToll/IL-1 Receptor adaptor protein (TIRAP Human)
UseRetroviralTags3x HA, 3x TEV, and BirA target siteExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only