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Plasmid#118361PurposeThis plasmid expresses the full length human SUMO1.DepositorInserthSUMO1 (SUMO1 Human)
UseTagsHISExpressionMammalianMutationPlease see Depositor CommentsPromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Gal4-VP16-HSF1 WT
Plasmid#71726Purposeplasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1 WT RDDepositorInsertGal4 DBD HSF1 VP16 AD (HSF1 Human, Budding Yeast, Herpes Simplex Virus protein VP16)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
hMEF2A K403R
Plasmid#118355PurposeThis plasmid expresses human MEF2A K403R mutant. This construct cannot undergo sumoylation on K403.DepositorInserthMEF2A (MEF2A Human)
UseTagsmyc and 6xHISExpressionMammalianMutationK403RPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
hHSF1 RNAi resistant
Plasmid#118347PurposeThis plasmid expresses RNAi resistance version of hHSF1 to use for rescue experiments with HSF1 shRNA vector (plasmid #118345).DepositorInserthHSF1 (HSF1 Human)
UseTagsmyc and 6xHISExpressionMammalianMutationRNAi resistance mutations (does not change amino …PromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
Gal4-VP16-HSF1Δ∼PRD
Plasmid#71727Purposeplasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1Δ∼PRD RDDepositorInsertGal4 DBD HSF1 Δ∼PRD RD VP16 AD (HSF1 Human, Budding Yeast, Herpes Simplex Virus protein VP16)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Gal4-HSF4b K294R
Plasmid#118359PurposeThis plasmid expresses amino-acids 200-493 of hHSF4b K294R mutant fused to the GAL-4 DNA binding domain. This construct cannot undergo sumoylation on K294.DepositorInserthHSF4b (HSF4 Human)
UseTagsExpressionMammalianMutationK294RPromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
hHSF4b K294R
Plasmid#118357PurposeThis plasmid expresses human HSF4b K294R mutant, which cannot undergo phosphorylation-dependent sumoylation.DepositorInserthHSF4b (HSF4 Human)
UseTagsExpressionMammalianMutationK294RPromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Gal4-HSF4b WT
Plasmid#118358PurposeThis plasmid expresses amino-acids 200-493 of hHSF4b WT fused to the GAL-4 DNA binding domain.DepositorInserthHSF4b (HSF4 Human)
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
hHSF1 S307A
Plasmid#118364PurposeThis plasmid expresses human HSF1 S307A mutant.DepositorInserthHSF1 (HSF1 Human)
UseTagsmyc and 6xHISExpressionMammalianMutationS307APromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
nuclear EKAR (Cerulean-Venus)
Plasmid#18681DepositorInsertERK Activity Reporter
UseTagsmonomeric Cerulean and monomeric VenusExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cerulean3-Cerulean3-FLARE-Cameleon
Plasmid#123353PurposeCyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.DepositorInsertCerulean3-Cerulean3-FLARE-Cameleon
UseTags6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cerulean3-Cerulean3-FLARE-CKAR
Plasmid#123346PurposeCyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity.DepositorInsertCerulean3-Cerulean3-FLARE-CKAR
UseTags6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cerulean3-Cerulean3-FLARE-EKAR-EV
Plasmid#123342PurposeCyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity.DepositorInsertCerulean3-Cerulean3-FLARE-EKAR-EV
UseTags6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-Cerulean3-Cerulean3-FLARE-D1ER
Plasmid#123354PurposeCyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator, targeted to endoplasmic reticulum lumen.DepositorInsertcp-moxCerulean3-moxCerulean3-FLARE-D1ER
UseTagsC-terminal ER retention signal (KDEL), cp-moxCeru…ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cerulean3-cpCerulean3-FLARE-AKAR
Plasmid#123336PurposeCyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Cerulean3/cpCerulean3-E173 homoFRET pair.DepositorInsertCerulean3-cpCerulean3-FLARE-AKAR
UseTags6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cerulean3-Cerulean3-FLARE-AKAR
Plasmid#123335PurposeCyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.DepositorInsertCerulean3-Cerulean3-FLARE-AKAR
UseTags6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET mCerulean LIC cloning vector (H6-mCerulean)
Plasmid#29726DepositorTypeEmpty backboneUseTagsBiotin - His6 - TEV and mCeruleanExpressionBacterialMutationPromoterAvailable sinceNov. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET mCerulean LIC cloning vector (u-mCerulean)
Plasmid#29773DepositorTypeEmpty backboneUseTagsmCeruleanExpressionBacterialMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CB7.CI.mCerulean.WPRE.RBG (AAV1)
Viral Prep#105557-AAV1PurposeReady-to-use AAV1 particles produced from pENN.AAV.CB7.CI.mCerulean.WPRE.RBG (#105557). In addition to the viral particles, you will also receive purified pENN.AAV.CB7.CI.mCerulean.WPRE.RBG plasmid DNA. CB7-driven mCerulean expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCB7TagsmCeruleanAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CB7.CI.mCerulean.WPRE.RBG (AAV9)
Viral Prep#105557-AAV9PurposeReady-to-use AAV9 particles produced from pENN.AAV.CB7.CI.mCerulean.WPRE.RBG (#105557). In addition to the viral particles, you will also receive purified pENN.AAV.CB7.CI.mCerulean.WPRE.RBG plasmid DNA. CB7-driven mCerulean expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCB7TagsmCeruleanAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only