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Plasmid#208377PurposeEpisomal plasmid for expression of 2 proteins with puromycin selection and EGFP monitoringDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterhuman CMVAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only
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pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-nTermPuroR-nTermIntein
Plasmid#208378PurposeEpisomal plasmid - for expression of 2 proteins linked with EGFP and n-terminal split puromycin resistance and split inteinDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterhuman CMVAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
IRES-mCherry
Plasmid#80139Purposeempty retroviral vector with IRES-mCherryDepositorTypeEmpty backboneUseRetroviralTagsIRES-mCherryExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tagged-IRES
Plasmid#35570DepositorInsertLymphoid Enhancer Factor 1 5'UTR (LEF1 Human)
UseLuciferaseTagsFirefly Luciferase and MS2 Stem LoopExpressionMammalianMutationPromoterSV40Available sinceApril 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
CVB3 IRES
Plasmid#189968PurposePart 2 plasmid to introduce the CVB3 IRES into circRNAsDepositorInsertCVB3 IRES
UseCloningTagsExpressionMutationPromoterN/AAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
IRE-SunTag
Plasmid#196920PurposeThe 5UTR of the ferritin heavy chain mRNA was cloned upstream of the SunTag-Renilla coding sequence in the ΔIRE-SunTag reporterDepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
GTH-ir
Plasmid#81107PurposeGene Tagging vector HIS/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)DepositorInserttdiRFP
UseCre/LoxTagstdiRFPExpressionYeastMutationPromoterAvailable sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
IRE-TREAT
Plasmid#196921PurposeThe 5UTR of the ferritin heavy chain mRNA was cloned upstream of the Renilla coding sequence in the ΔIRE-TREAT reporterDepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFloxin-IRES
Plasmid#24554DepositorTypeEmpty backboneUseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
GTL-ir
Plasmid#81102PurposeGene Tagging vector LEU/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)DepositorInserttdiRFP
UseCre/LoxTagstdiRFPExpressionYeastMutationcodon optimized second iRFP to avoid internal rec…PromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
GTT-ir
Plasmid#81112PurposeGene Tagging vector TRP/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)DepositorInserttdiRFP
UseCre/LoxTagstdiRFPExpressionYeastMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmVen-IRES
Plasmid#211428PurposeVector backbone for coupled mammalian expression of N-terminal Venus fusion protein and another proteinDepositorInsertIRES sequence (RPA2 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
GTU-ir
Plasmid#81097PurposeGene Tagging vector URA/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)DepositorInserttdiRFP
UseCre/LoxTagstdiRFPExpressionYeastMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMS22H-IRE
Plasmid#133905PurposePositive control when used in combination with pAWH-IRP. Expression of MS2BS-IRE site hybrid. Homology regions for recombination with pAWHDepositorInsertIRE (Ftl1 Rat)
UseTagsExpressionYeastMutationPromoterPol IIIAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF)
Plasmid#101139PurposeLuciferase reporter plasmid. Note that both renilla luciferase and ORF-firefly luciferase are encoded by a single mRNA and both are translated independently via internal ribosomal binding sites.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
G718A_BattB(GA)_TKterm_AttB(GT)_sGFP-PTEN-IRES-mCherry-P2A-HygroR_noTerm_attP(GA)_IRES-UnaG-2A-Puro-Term
Plasmid#200634PurposePlasmid self-excising recombination plasmid for Matreyek Bxb1(GT) landing padDepositorInsertPTEN (PTEN Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBlue-ACTB-IR-IRES-Puro-pEF1α-EGFP-IR-ACTB
Plasmid#169921PurposeDNA donor plasmid for site-specific integration of IRES-Puro-pEF1a-EGFP cassette at the ACTB locus using Cas9-transposase fusion proteins.DepositorInsertIRES-Puro-pEF1a-EGFP
UseCRISPRTagsExpressionMutationPromoterEF1aAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCV_IRES_GFP_CD16a
Plasmid#196189PurposeRetroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.DepositorInsertCD16a (FCGR3A Human)
UseRetroviralTagsExpressionMammalianMutationchanged Phenylalanine 158 to ValinePromoterLTRAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
PH-PLCD1_mScarlet_IRES_SYFP2_PH_N1
Plasmid#85069PurposeYellow/red FRET biosensor for monotoring plasmamembrane PtdIns(4,5)P2 levels (PIP2)DepositorInsertPH-PLCD1 (PLCD1 Human)
UseTagsIRES-sYFP2-PH and mScarletExpressionMammalianMutationPLCD aa 1–170PromoterCMVAvailable sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.Luc.IRES.EGFP.SV40
Plasmid#105533PurposeAAV expression of Luciferase and EGFP from CMV promoterDepositorInsertLuciferase
UseAAV and LuciferaseTagsIRES-GFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only