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Plasmid#233596PurposeExpression of NFAST on the ER membraneDepositorInsertNFAST
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
ER-frNFAST
Plasmid#233599PurposeExpression of frNFAST on the ER membraneDepositorInsertfrNFAST
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
PM-frNFAST
Plasmid#233602PurposeExpression of frNFAST on the PM facing the cytosolDepositorInsertfrNFAST
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFastBacHTa_LYN
Plasmid#224244PurposeExpression of human LYN Kinase in insect cells (SF9 cells)DepositorInsertlyn (LYN Human)
UseTags6xHis-TEVExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFastBacHTa_SYK
Plasmid#224246PurposeExpression of human SYK Kinase in insect cells (SF9 cells)DepositorInsertsyk (SYK Human)
UseTags6xHis-TEVExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFast-hRad1
Plasmid#222471PurposeExpresses hRad1 in insect cellsDepositorInserthRad1 (RAD1 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFast-hHus1
Plasmid#222469PurposeExpresses hHus1 in insect cellsDepositorInserthHus1 (HUS1 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-His10FLAG
Plasmid#157736Purposeempty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sitesDepositorTypeEmpty backboneUseTagsHis10 + 3xFLAG, enterokinase-cleavableExpressionInsectMutationPromoterpolyhedrinAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-pGC-A
Plasmid#186626PurposepFastBac1 (baculovirus polyhedrin promoter) encoding HA signal peptide, TEV-protease cleavable His10-tag, and full-length human pGC-A (NP_000897.3 amino acids 33-1061; expression-optimized DNA)DepositorInsertNPR1 (NPR1 Human)
UseTagshemagglutinin (HA) signal peptide + His10-tag + T…ExpressionInsectMutationdeleted amino acids 1-32PromoterpolyhedringAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-Dual-M2
Plasmid#135584PurposeHigh eukaryotic dual protein expression shuttle vectorDepositorTypeEmpty backboneUseTransfer vectorTagsExpressionInsectMutationPromoterpolyhedrin of AcMNPVAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG654 pFAST
Plasmid#172861PurposeExpresses pFAST in mammalian cellsDepositorInsertpFAST
UseTagsMyc tag (C terminal on insert)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-His10TEV
Plasmid#159427Purposeempty pFastBac1 vector (polyhedrin promoter) adds TEV-protease-removable N-terminal His10-tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sitesDepositorTypeEmpty backboneUseTagsTEV-protease-cleavable His10-tagExpressionInsectMutationPromoterpolyhedrinAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual_SNF2L-opt_p48
Plasmid#102243PurposeExpression of human codon optimized human SNF2L and pRbAp48 from pFastBac dualDepositorUseTagsExpressionInsectMutationcodon optimized for expressionPromoterPolyhedrin and p10Available sinceNov. 1, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223-FASTKD5
Plasmid#23560DepositorInsertFASTKD5 (FASTKD5 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAG244 iFAST
Plasmid#130809PurposeExpresses iFAST in mammalian cellsDepositorInsertiFAST
UseTagsHis-tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRE-Fast-FT
Plasmid#31913DepositorInsertFast-FT
UseTagsExpressionMammalianMutationM18V/E34K/K69R/L84W/S149T/A224S compared to mCher…PromoterPTight Tet-responsive PminCMVdAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEND-164_Cacnes_pFAST
Plasmid#225616PurposeReplicative vector for anaerobic fluorescent reporter protein FAST tag strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+pFAST.DepositorInsertpFAST
UseSynthetic BiologyTags6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1_FLAG-BPTF
Plasmid#102242PurposeExpression of human Flag-BPTF using baculovirus from pFastBac1DepositorInsertFlag-BPTF (BPTF Human)
UseTagsFlagExpressionInsectMutationPromoterPolyhedronAvailable sinceDec. 11, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAG364-greenFAST
Plasmid#160353Purposeexpresses greenFAST in mammalian cellsDepositorInsertgreenFAST
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG365-redFAST
Plasmid#160354PurposeExpresses redFAST in mammalian cellsDepositorInsertredFAST
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only