Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

PiggyBac vector to express GA-inducible VPR-Sa_dCas9 with hygromycin resistance

Ready-to-use Lentiviral Prep particles produced from Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A (#92379). In addition to the viral particles, you will also receive purified Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR activation screening in human cells. Contains 56,762 sgRNAs, targeting 18,885 genes. Concentrated lentiviral particles carrying set A of the Calabrese human CRISPRa sgRNA activation library in backbone XPR_502 (P65 HSF) .

AAV-CRISPRa base vector

AAV-CRISPRa base vector

CRISPR/Cas9 to tag up to 7 different genomic locations. Unique labeling system allows scientists to track DNA movement in live cells and study the structure of the genome in real time.

Expression of gRNA targeting POGZ for CRISPRa; U6 promoter

aTc inducible MCP-SoxS double mutant expression for CRISPRa in E.coli. Contains constitutive dCas9 and scRNA expression

PiggyBac vector for expression of 1x gRNA targeting POGZ for CRISPRa;mRFP-T2A-BlasticidinR

Expression of gRNA a1 targeting TCF4 for CRISPRa; U6 promoter

PiggyBac vector for expression of 1x gRNA targeting TCF4 for CRISPRa;mRFP-T2A-BlasticidinR

PiggyBac vector for expression of 3xgRNA targeting TCF4 for CRISPRa; mRFP-T2A-BlasticidinR

Expression of gRNA a3 targeting TCF4 for CRISPRa; U6 promoter

Expression of gRNA a2 targeting TCF4 for CRISPRa; U6 promoter

reporter circuit (PpspA-2G6 with sfgfp::ASV)

Ef1a promoter

Entry vector for multi-gRNA cloning

mRFP with synthetic promoter HP3 (from Figure S21)

J23106.EL222