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Plasmid#125590PurposeOverexpression plasmid for production of WcbL from Burkholderia pseudomalleiDepositorInsertBpWcbL (wcbL Burkholderia pseudomallei)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDAI-SceI-pheS
Plasmid#111611PurposeYeast homing endonuclease I - SceI expression vector. Used for resolution of merodiploids during allelic exchange in Burkholderia sp.DepositorInsertpheS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC201
Plasmid#129387PurposeA variant of the pSCrhaB2 vector with the oriR6K and mob genes from pGPΩ-TP. Used for homologous recombination of the rhamnose-inducible system into the chromosome of Burkholderia cenocepacia K56-2.DepositorInsertoriR6K and oriT
UseTagsExpressionBacterialMutationN/APromoterN/AAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRhaCAST
Plasmid#211791PurposepZLrhaB2plus with Sh-cas12K-tnsBC-tniQ cloned into, which drives by the rhamnose inducible promoterDepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning
UseCRISPRTagsExpressionBacterialMutationPromoterRhamnose-inducibleAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYpKdoO
Plasmid#36284DepositorInserty1812
UseTagsExpressionMutationPromoterAvailable sinceJuly 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBaKdoO
Plasmid#36285DepositorInsertbamb_0774
UseTagsExpressionMutationPromoterAvailable sinceJuly 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pYpKdoO.1
Plasmid#36286DepositorInserty1812
UseTagsExpressionMutationPromoterAvailable sinceMay 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMLS7-egfp
Plasmid#112917PurposeFluorescence microscopyDepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterPS7 promoter and Ps7Available sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMLBAD-eGFP
Plasmid#112918PurposeFluorescence microscopyDepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterPbadAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTNS3
Plasmid#63127PurposeTn7 transposase expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterP1 and PtacAvailable sinceMay 17, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSC201plus
Plasmid#164227PurposeIntegrative plasmid to introduce modified rhamnose-inducible promoter into bacterial genomes. Harbours enhanced rhaS binding site and encodes T7 gene10 stem loop in mRNA for enhanced expressionDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterRhamnose-inducibleAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDAI-SceI
Plasmid#32061PurposeConstruction of unmarked gene deletions. Broad host range replicative plasmid expressing the I-SceI homing endonucleaseDepositorInsertI-SceI nuclease
UseTagsExpressionBacterialMutationPromoterPdhfrAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDA12
Plasmid#113663PurposeCloning vector for bacterial expression with Pdhfr promoterDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPdhfrAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a-BpeR
Plasmid#51159DepositorInsertBpeR
UseSynthetic BiologyTags6x-hisExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSCB2-sgRNA
Plasmid#129463PurposeTemplate plasmid for guide sequence insertion via inverse PCR. Derived from pSCrhaB2-gRNA (see paper).DepositorInsertpgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 (SpeI)Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDAI-SceI-SacB
Plasmid#113635PurposeBroad host range replicative plasmid expressing the I-SceI homing endonuclease and the counterselectable marker SacBDepositorInsertsacB
UseTagsExpressionBacterialMutationPlease see Depositor CommentsPromotersacB promoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSCrhaB2plus
Plasmid#164226PurposeBroad-range bacterial expression vector with modified rhamnose-inducible promoter. Harbours enhanced rhaS binding site and encodes T7 gene10 stem loop in mRNA for enhanced expression.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterRhamnose-inducibleAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAH25-SceI
Plasmid#129389PurposeDerived from pDAI-SceI. Tetracycline resistance removed and replaced with chloramphenicol resistance cassette from pKD3.DepositorInsertChloramphenicol acetyltransferase
UseTagsExpressionBacterialMutationN/APromoterCAT promoterAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAH-CTX1-rha
Plasmid#129390PurposeDerived from mini-CTX1. An integrase vector that can integrate the rhamnose-inducible promoter system into the chromosome of a host bacterium.DepositorInsertRhamnose-inducible promoter
UseTagsExpressionBacterialMutationN/APromoterPrhaBADAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-guideless
Plasmid#129464PurposeDerived from pSCB2-sgRNA. 20 nucleotide binding sequence of sgRNA removed by inverse PCR. Used as a negative control.DepositorInsertGuideless gRNA backbone
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 (SpeI)Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only