-
Plasmid#164855Purposeconstitutive TIR1 expression vector for mouse H11 locusDepositorInsertOsTIR1
UseTagsHA tagExpressionMammalianMutationPromoterEF1-alphaAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-Ostir-neo
Plasmid#161973PurposeExpresses Ostir and G418 resistance gene in mammalian cellsDepositorInsertOstir-3xHA-p2a-neoR
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOsTIR1w/oGFP
Plasmid#102883PurposeExpresses OsTIR1 via the ADH1 promoter in yeast. The cassette is integrated in the HO locus. Assembly and characterization of the auxin-inducible degradation.DepositorInsertOsTIR1
UseTags3x mycExpressionYeastMutationPromoterADH1Available sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-TIR1-HA
Plasmid#172610PurposeLentiviral vector for the Tet-inducible expression of HA-tagged TIR1 and a constitutive puromycin resistance marker in mammalian cellsDepositorInsertTIR1
UseLentiviralTagsHAExpressionMammalianMutationPromoterTet/ON Minimal CMV promoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Punc-4c.TIR1
Plasmid#194053PurposeTIR1 expression vector under the unc-4c promoter expressed in the DA motor neurons in C. elegansDepositorInsertTIR1 (TIR1 Mustard Weed)
UseTagsExpressionWormMutationcodon-optimized for expression in C. elegans; Two…Promoterunc-4cAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOpen-PstIR
Plasmid#165526PurposeRestriction enzyme that recognizes the double-stranded sequence CTGCAG and cleaves after A-5.DepositorInsertPstI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
PB-Ostir-puro
Plasmid#161972PurposeExpresses Ostir and puromycin resistance gene in mammalian cellsDepositorInsertOstir-3xHA-p2a-puroR
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiRCas9-Lambda2
Plasmid#104184PurposeLentiviral transfer vector that carries U6-driven non-targeting sgRNA using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)DepositorInsertU6-sgRNA, EFS-PIN-dCas9
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
TIRAP-GFP
Plasmid#52739PurposeRetroviral vector expresses WT hTIRAPfused to GFP. Also contains IRES-hCD2 as selectable markerDepositorInsertToll/IL-1 Receptor adaptor protein (TIRAP Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTRE-BI-osTIR1
Plasmid#207840PurposeInducible TIR1 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cellsDepositorInsertsosTIR
mCherry
mAID_-VHH-GFP4
UseTags3X HA-Tag and weak NLSExpressionMammalianMutationAll K mutated to RPromoterTRE3G BI promoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only