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pcDNA3.1-mPCSK9
Plasmid#122678PurposeExpresses mouse PCSK9 in mammalian cellsDepositorInsertMus musculus proprotein convertase subtilisin/kexin type 9 (Pcsk9) (Pcsk9 )
UseTagsExpressionMammalianMutationPromoterT7Available sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC5
Plasmid#118863Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C proteaseDepositorInsertNcoI-HIS6-3C-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
His-DERA
Plasmid#203225PurposeFor protein expression. E. coli 2-deoxy-D-ribose-5-phosphate aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.DepositorInsertdeoxyribose aldolase
UseTagsN-terminal TEV cleavable 6xHis tagExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET26b-Granzyme A (S-A)
Plasmid#8825DepositorInsertHuman Granzyme A inactive (GZMA Human)
UseTags(His)6ExpressionBacterialMutationchanged Serine 184 to AlaninePromoterAvailable sinceJune 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-PTDSS1-IRES-Puromycin
Plasmid#134667PurposeExpresses EGFP-tagged Mitochondria-associated ER membrane markerDepositorInsertPTDSS1 (PTDSS1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS/ES-EnvA-VSVg-WPRE-pA
Plasmid#87221Purposeto package wild type EnvA psudotyped lentivirusDepositorInsertEnvA-VSVg
UseMouse Targeting and Synthetic BiologyTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHA-mLRH-1
Plasmid#16342DepositorInsertLRH-1 (Nr5a2 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-C3-HA-Ubiquitin
Plasmid#28236DepositorInsertUbiquitin (UBB Human)
UseTagsEYFP and HAExpressionMammalianMutationPromoterAvailable sinceApril 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
MSCV miRNA control plasmid
Plasmid#31508DepositorTypeEmpty backboneUseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMSC236
Plasmid#216368PurposexhNup155-Nb2i (+2Cys) Nanobody productionDepositorInsertxhNup155-Nb2i (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC235
Plasmid#216372PurposexhNup155-Nb3i (+2Cys) Nanobody productionDepositorInsertxhNup155-Nb3i (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC227
Plasmid#216351PurposexhNup35-Nb1t (+3Cys) Nanobody productionDepositorInsertxhNup35-Nb1t (+3Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-LBR-EGFP-polyA
Plasmid#59748PurposeExpress LBR-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.DepositorInsertLBR-EGFP
UseTagsEGFPExpressionMammalianMutationPromoterCMV and T7Available sinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
ND4.2-Right DdCBE-G1397-C- T1413I
Plasmid#179685PurposeExpression of base editor in mammalian cellsDepositorInsertSOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_SOD2 3'UTR
UseTagsExpressionMammalianMutationT1413IPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDAI-SceI-SacB
Plasmid#113635PurposeBroad host range replicative plasmid expressing the I-SceI homing endonuclease and the counterselectable marker SacBDepositorInsertsacB
UseTagsExpressionBacterialMutationPlease see Depositor CommentsPromotersacB promoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRG714_lexO-AscI_LexA-TF_URA3MX
Plasmid#154822PurposeInducible expression of AscI endonuclease in S. cerevisiaeDepositorInsertsAscI endonuclease
LexA-ER-B112
UseTagsFLAG and NLSExpressionYeastMutationPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-V5-hTEV
Plasmid#65800Purposeexpresses human codon-optimised TEV proteaseDepositorInsertV5-human TEV
UseTagsV5ExpressionMammalianMutationcodon-optimised to express in mammalian cellsPromoterCMVAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSCL.139
Plasmid#184994PurposeExpress Cas9-P2A-Eco1RT in human cellsDepositorInsertSpCas9-P2A-Eco1RT
UseTagsSV40NLSExpressionMammalianMutationHuman codon optimized RTPromoterTetOn-3gAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_BIRC6
Plasmid#197974PurposeEntry clone for wt BIRC6DepositorInsertBIRC6 (BIRC6 Human)
UseOtherTagsExpressionMutationL1332VPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only
