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Plasmid#79747PurposeThis plasmid encodes the kinase domain of FAK2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertFAK2 (PTK2B Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nef-CIAO-Flp
Plasmid#149294PurposeCre-dependent expression of Flp recombinase.DepositorInsertFlp recombinase (flp Budding Yeast)
UseAAVTagsExpressionMutationDerived from the mouse codon-optomized FlpO. The…PromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEMS2022
Plasmid#49129PurposeAAV plasmid with DBH promoter (PRS2/3:Con) driving expression of iCre.DepositorInsertssAAV-PRS2/3:Con-iCre
UseAAVTagsExpressionMammalianMutationPromoterDBHAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
Cav2.2 pcDNA3
Plasmid#206072Purposeexpression of rabbit Cav2.2 calcium channel, the 3'UTR is deleted, this enhances the expressionDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF myc Gal cyclin T1 (1-726) (P#1026)
Plasmid#14626DepositorInsertcyclin T1 (CCNT1 Human)
UseTagsGal and mycExpressionMammalianMutationPromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
1477 pcDNA3 flag HA Akt1
Plasmid#9021DepositorInsertAkt1 (AKT1 Human)
UseTagsHA and flagExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pEMS1994
Plasmid#49124PurposeAAV plasmid with S100B (Ple266) promoter driving expression of iCre.DepositorInsertssAAV-Ple266-iCre
UseAAVTagsExpressionMammalianMutationPromoterS100BAvailable sinceMay 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
dCas9-dMSK1
Plasmid#165602PurposeExpresses Sp dCas9 fused to truncated human MSK1 (42-802)DepositorInsertS. Pyogenes dCas9 with c-terminal truncated human Mitogen- and stress-activated protein kinase-1 (42-802) (RPS6KA5 Synthetic, S. Pyogenes, Human)
UseCRISPR and LentiviralTagsFLAG TagExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis-TagRFP-T_A1aY1
Plasmid#158754PurposeExpression of 6x His tagged TagRFP-T_A1aY1 in mammalian cells for detection of tyrosinated microtubules.DepositorInsert6xHistidine_TagRFP-T_A1aY1
UseTags6x-Histidine tag and TagRFP-TExpressionMammalianMutationPromoterCMV PromoterAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEarleyGate 100RMOA mPing
Plasmid#196137PurposeMeasures mPing transposition in plantsDepositorInsertsPong TPase LA T2A ORF1SC1 ONE
mPing
UseBinaryTagsExpressionPlantMutationTPase NES sequence mutated L418A, L420A; T2A pept…Promoter35S and RPS5aAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAC8_MF-p10-mCh (VE5626)
Plasmid#139772PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with mCherry cDNA under p10 to monitor infection.DepositorInsertmCherry fluorescent protein
UseTagsExpressionMutationPromoterPHAvailable sinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pES002 MBP-GSK3β_S9A-HA-His
Plasmid#196184PurposeExpresses MBP-GSK3β_S9A-HA-His (human Glycogen Synthase Kinase 3β S9A mutant as a fusion protein with MBP, HA, and His- tags) in E.coliDepositorInsertGSK3B (GSK3B Human)
UseTagsHA, His6, and MBPExpressionBacterialMutationchanged Serine 9 to AlaninePromoterTacAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
Plxna1-Fc-His
Plasmid#72122PurposeExpresses the extracellular region of the PlexinA1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertPlxna1 (Plxna1 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-RYK-V5/HIS
Plasmid#205610PurposeExpression of human RYK receptor tyrosine kinase in mammalian cellsDepositorInsertRYK receptor tyrosine kinase (RYK Human)
UseTagsV5-HisExpressionMammalianMutationPromoterCMVAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBSFI-myr-akt-T308D/S473D
Plasmid#49192PurposecDNA of Akt1 with myristyolation signal and point mutation from T to D at position 308 and S to D at position 473 in cloning vectorDepositorInsertAkt1 (Akt1 Mouse)
UseAdaptor plasmidTagsHA tag and myristolation signalExpressionMutationT308D; S473DPromoterT7Available sinceDec. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-ExRai-AKAR2(T/A)
Plasmid#161754PurposeNegative-control mutant for ExRai-AKAR2 biosensor.DepositorInsertExRai-AKAR2(T/A)
UseTagsExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
Plxna2-Fc-His
Plasmid#72123PurposeExpresses the extracellular region of the PlexinA2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertPlxna2 (Plxna2 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
SLK_HUMAN_D0
Plasmid#79733PurposeThis plasmid encodes the kinase domain of SLK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertSLK (SLK Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
2xmyc-JIP4
Plasmid#164633PurposeExpresses JIP4 in mammalian cells with a 2xmyc tagDepositorInsertJIP4 (SPAG9 Human)
UseTags2xmycExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-ATR-C-KD
Plasmid#53770Purposemammalian expression of flag-tagged ATR C-terminal fragment kinase dead mutantDepositorInsertATR (ATR Human)
UseTagsFlagExpressionMammalianMutationC-terminal domain, aa 1398-2644, and kinase dead …PromoterCMVAvailable sinceJuly 8, 2014AvailabilityAcademic Institutions and Nonprofits only