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Showing: 3921 - 3940 of 4745 results

  5. pYI2165

    Plasmid
    #228347
    Purpose
    2,4-Diacetylphloroglucinol (DAPG)-regulatable ALX-0171 expression
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    MFalpha(2xAdv)-LE-ALX-0171-3A-FLAG-His
    Use
    Affinity Reagent/ Antibody
    Tags
    AAA linker followed by FLAG-His6 and MF_ secretio…
    Expression
    Yeast
    Mutation
    Promoter
    2,4-Diacetylphloroglucinol-regulatable synthetic …
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228347-plasmid-map-sequence-id-457108

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  6. pYI2216

    Plasmid
    #228349
    Purpose
    2,4-Diacetylphloroglucinol (DAPG)-regulatable ALX-0081 expression
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    MFalpha(2xAdv)-LE-ALX-0081-3A-FLAG-His
    Use
    Affinity Reagent/ Antibody
    Tags
    AAA linker followed by FLAG-His6 and MF_ secretio…
    Expression
    Yeast
    Mutation
    Promoter
    2,4-Diacetylphloroglucinol-regulatable synthetic …
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228349-plasmid-map-sequence-id-457093

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  9. pTFG027_Spike_sACE2-resistant_D614G_F486S_3xFlag

    Plasmid
    #191572
    Purpose
    Mutated SARS-CoV-2 Spike under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. Encodes F486S mutation. For expression and pseudotyping lentiviral particles.
    Depositor
    Joshua Leonard
    Article
    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10
    Insert
    SARS-CoV-2 Spike Protein (S Synthetic)
    Use
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    D614G and F486S (confers reduced binding affinity…
    Promoter
    CMV
    Available since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191572-plasmid-map-sequence-id-441975

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  12. pcDNA3.1(+)Gα12-133-NLuc

    Plasmid
    #207156
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA12 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
    Depositor
    Dominique Schols, Tom Van Loy
    Article
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Insert
    GNA12 (GNA12 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    NLuc inserted at position A(133)F
    Promoter
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207156-plasmid-map-sequence-id-410281

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  15. Cav2.2 BBS W391A pcDNA3

    Plasmid
    #206077
    Purpose
    Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop that prevents Beta binding
    Depositor
    Annette Dolphin
    Article
    Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11
    Insert
    cacna1b (CACNA1B Rabbit)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    W391A
    Promoter
    CMV
    Available since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    206077-plasmid-map-sequence-id-406768

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  20. pNOC_dCas9_sgRNA Td1

    Plasmid
    #176257
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 1
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176257-plasmid-map-sequence-id-347030

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Showing: 3921 - 3940 of 4745 results