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Showing: 3921 - 3940 of 4616 results

  2. pcDNA5/FRT/TO-GAlphaGustducin-RLuc8

    Plasmid
    #140979
    Purpose
    Encodes a G alpha subunit (GNAT3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphaGustducin-RLuc8 (GNAT3 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140979-plasmid-map-sequence-id-300662

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  6. pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899,884 (All)

    Plasmid
    #194190
    Purpose
    Includes the promoter (1kb) of SMTS with 5 mutated KLF binding sites (positions 449,548,732,899,884)
    Depositor
    Matthias Leisegang, Ralf Brandes
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    5 mutated KLF binding sites (positions 449,548,73…
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194190-plasmid-map-sequence-id-381947

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  7. pGL3-pSMANTIS_1kb_KLF2mut 899

    Plasmid
    #194193
    Purpose
    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 899)
    Depositor
    Matthias Leisegang, Ralf Brandes
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    mutated KLF binding sites (position 899)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194193-plasmid-map-sequence-id-381931

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  8. pINSPECT_5'-xrRNA_NLuc_3'-XAP1

    Plasmid
    #197264
    Purpose
    Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' XAP1). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Type
    Empty backbone
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    197264-plasmid-map-sequence-id-389660

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  11. pNOC_dfnCas12a-KRAB_crRNA NC

    Plasmid
    #176262
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and spacer sequence is replaced by the type IIS restriction site for endonucleas
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Expression
    Mutation
    D917A and E1006A mutations to inactivate the endo…
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176262-plasmid-map-sequence-id-346962

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  12. Bxb1Donor_G102H_ADRB2_LucReporter

    Plasmid
    #129794
    Purpose
    encodes a donor vector for recombination with the Bxb1 recombinase with variant G102H of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene
    Depositor
    Sriram Kosuri
    Article
    Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1
    Insert
    ADRB2 (ADRB2 Human)
    Use
    Synthetic Biology
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    G102H
    Promoter
    N/A
    Available since
    Sept. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    129794-plasmid-map-sequence-id-255134

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Showing: 3921 - 3940 of 4616 results