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Viral Prep#112004-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSynapsin1-axon-GCaMP6s-P2A-mKate2 (#112004). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-axon-GCaMP6s-P2A-mKate2 plasmid DNA. Synapsin-driven, axon-targeted GCaMP6s calcium sensor and bicistronic mKate2 expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmKate2Available sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only
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pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (AAV8)
Viral Prep#154867-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (#154867). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterHuman Synapsin-1TagsmCherry (Flp-dependent)Available sinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
CRISPRecise Kit
Plasmid Kit#1000000235PurposeCRISPRecise is a set of high-fidelity SpCas9 variants that can be used to efficiently edit any SpCas9 target with practically no detectable off-target effects.DepositorAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (AAV Retrograde)
Viral Prep#154867-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (#154867). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterHuman Synapsin-1TagsmCherry (Flp-dependent)Available sinceOct. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJH-MBP-CbAgo
Plasmid#127707PurposepET His6 MBP TEV CbAgoDepositorInsertCbAgo
UseTagsMPBExpressionBacterialMutationPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLD1 translation factors
Plasmid#117760PurposeBioBrick encoding cistrons 1-13 in pET for preparation of PURE 3.0 translation system from E. coliDepositorInsertTranslation factor cistrons 1-13
UseEscherichia coliTagsExpressionMutationPromoterAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLD3 translation factors
Plasmid#117762PurposeBioBrick encoding cistrons 22-30 in pET for preparation of PURE 3.0 translation system from E. coliDepositorInsertTranslation factor cistrons 22-30
UseEscherichia coliTagsExpressionMutationPromoterAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLD2 translation factors
Plasmid#117761PurposeBioBrick encoding cistrons 14-21 in pET for preparation of PURE 3.0 translation system from E. coliDepositorInsertTranslation factor cistrons 14-21
UseEscherichia coliTagsExpressionMutationPromoterAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
(+)-Limonene Synthase
Plasmid#112230Purpose(+)-Limonene Synthase from Citrus sinensis in pET-28a(+). N-terminal was truncated to remove amino acids 1-52 and then His-tagged for purification.DepositorInsert(+)-Limonene Synthase
UseTagsN-terminal His-tagExpressionBacterialMutationdeleted amino acids 1-52PromoterAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBz15_Strep_Rubisco
Plasmid#140839Purposewild-type Halothiobacillus neapolitanus Form I Rubisco with StrepII tag (CbbL-StrepII, CbbS), pET-14b backboneDepositorInsertForm IA Rubisco; CbbL, CbbS
UseTagsStrep IIExpressionBacterialMutationPromoterT7Available sinceJune 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
S019
Plasmid#174552PurposepET-iBclxL-v3, His tag for affinity purificationDepositorInsertiBclxL-v3, Bclxl inhibitor resistant protein
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
S330
Plasmid#174553PurposepET-iBcl2-v4, His tag for affinity purificationDepositorInsertibcl2-v4, bcl2 inhibitor resistant protein
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAP007
Plasmid#206809PurposepET-6xHis-MBP-TEV-BabTIR-APAZ/BabAgo - Heterologous BabSPARTA expressionDepositorInsertBabSPARTA operon (6xHis-MBP-BabTIR-APAZ and BabAgo)
UseTags6xHis-MBPExpressionBacterialMutationPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBz106_CsoS2_MR
Plasmid#140843PurposeHis-tagged CsoS2 Middle Region (MR), pET-14b backboneDepositorInsertCsoS2 Middle Region (MR)
UseTagsHexahistidine tagExpressionBacterialMutationonly amino acids 257-603PromoterT7Available sinceJune 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz109_CsoS2_NTD
Plasmid#140842PurposeHis-tagged CsoS2 N-terminal domain (NTD), pET-14b backboneDepositorInsertCsoS2 N-terminal domain (NTD)
UseTagsHexahistidine tagExpressionBacterialMutationdeleted amino acids 262-869PromoterT7Available sinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz47_polyPro_CsoS2_Npeptide
Plasmid#140845PurposeConsensus N*-peptide after polyproline II helix, N-terminal His- tag used as BLI bait, pET-14b backboneDepositorInsertpolyproline helix with CsoS2 N-peptide
UseTagsHexahistidine tag and Strep-tag IIExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz110_CsoS2_CTD
Plasmid#140844PurposeHis-tagged CsoS2 C-terminal domain (CTD), pET-14b backboneDepositorInsertCsoS2 C-terminal domain (CTD)
UseTagsHexahistidine tagExpressionBacterialMutationdeleted amino acids 1-594PromoterT7Available sinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCIOX
Plasmid#51300PurposeE.coli expression vector for His8-SUMO tagged recombinant proteinDepositorTypeEmpty backboneUseTagsHis8 + SUMOExpressionBacterialMutationPromoterT7Available sinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBz172_CsoS2_N1_GFP
Plasmid#140852PurposeHis-tagged first N-peptide of NTD fused to Superfolder GFP ([N1]-GFP) used for BLI and MST, pET-14b backboneDepositorInsertCsoS2 NTD repeat 1 fused to GFP
UseTagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz175_CsoS2_N4_GFP
Plasmid#140855PurposeHis-tagged fourth N-peptide of NTD fused to Superfolder GFP ([N4]-GFP) used for BLI, pET-14b backboneDepositorInsertCsoS2 NTD repeat 4 fused to GFP
UseTagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only