Expression of dCasMINI-V4-VPR-mCherry in mammalian cells

Synchronize trafficking of TNFalpha from the ER (RUCH system)

Lentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR

Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation

Expresses dSpyCas9-3XmCherry and telomere-targeting Spy sgRNA

nuclease inactive control for TRF1FokI WT

Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site A)

High-efficient mammalian expression vector for co-expression of BFP-, EGFP- and mCherry-tagged proteins using P2AT2A. MTS, NLS and PTS1 can be replaced by proteins of interest.

Expression of EACTB sensor in mammalian cells

Expression of dCas12f-VPR-mCherry in mammalian cells

Expression of a sensor for human DNAJB1 (hsp40) 3' UTR in mammalian cells; mCherry marker, EGFP output, three MS2 sequences in 3' UTR

CLIP donor template to generate 2 knock-ins: GFP into ACTB, and mCherry in RAB11A.

Expression of a sensor for GAPDH 3' UTR in mammalian cells; mCherry marker, EGFP output, three MS2 sequences in 3' UTR

Expression of MYC sensor in mammalian cells

Dual fluorescent reporter construct with single core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

Expression of a sensor for synthetic sequence 1 (EK0208) with three MS2 sequences in the 3' UTR in mammalian cells. Compared to EK0285, also has additional restriction sites around the sensor sequence. Recommended for the basis for cloning new sensors for use with MCP-ADAR(DD). HindIII & MluI can be used to swap out marker (mCherry). (KpnI or BamHI) and (BshTI or BcuI) can be used to swap out sensor sequence (s70587). XhoI and ApaI can be used to swap out output (EGFP).

Constitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPR

Mammalian expression vector for sgRNAs cloned into the BbsI sites and for expression of spCas9 linked to mCherry via a T2A peptide

heat shock promoter - activated hyperdCas12a-based transcriptional activator