Addgene

We narrowed to 14,719 results for: LEA

Showing: 3901 - 3920 of 14719 results

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pOCC153

Plasmid

#118901

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal TwinStrep, cleavable with 3C

Depositor

PEPC MPI CBG, Marc Bickle

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-TwinStrep-3C-NotI-ccdB-AscI-stop-HindIII cassette

Use

Tags

TwinStrep, cleavable with 3C protease

Expression

Insect

Mutation

Promoter

polH

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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Human SERCA3 (pMT2)

Plasmid

#75189

Purpose

Mammalian expression of ATP2A3

Depositor

Jonathan Lytton, David MacLennan

Article

Poch et al Am J Physiol. 1998 Dec;275(6 Pt 1):C1449

Insert

ATP2A3 (ATP2A3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

adenovirus major late promoter

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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ND4.2-Right DdCBE-G1397-C-wildtype

Plasmid

#179687

Purpose

Expression of base editor in mammalian cells

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C )_1xUGI_SOD2 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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HA.PKD.K/W

Plasmid

#10809

Depositor

Alex Toker

Article

Storz et al J Biol Chem. 2003 May 16. 278(20):17969-

Insert

PKD (PRKD1 Human)

Use

Tags

HA and Myc (inserted between amino acids 33&3…

Expression

Mammalian

Mutation

Kinase dead mutant: K612W (Lys 612 to Trp) (bp183…

Promoter

CMV

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pTP443

Plasmid

#216361

Purpose

xNup98-Nb1t (+1Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xNup98-Nb1t (+1Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV PIG IMP-1-short

Plasmid

#21659

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

IGF-II mRNA-binding protein 1 (IGF2BP1 Human)

Use

Retroviral

Tags

GFP and IRES

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pRevTRE MKL1-N100

Plasmid

#19848

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

Insert

MKL1-N100 (MRTFA Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mutation

deleted amino acids 1-100

Promoter

Available since

Jan. 13, 2009

Availability

Academic Institutions and Nonprofits only
pHR-TRE3G-PYL1-sfGFP-PML

Plasmid

#167011

Purpose

expression of PYL1-SfGFP-PML, lentivirus vector

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

PML (PML Human)

Use

Lentiviral

Tags

GFP-PML

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-p53 (NES-)

Plasmid

#12092

Depositor

Tyler Jacks

Article

Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

Insert

p53 (NES-) (TP53 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

L348A L350A Localized to nucleus.

Promoter

Available since

Aug. 7, 2006

Availability

Academic Institutions and Nonprofits only

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MSP1594

Plasmid

#65775

Purpose

Human expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLS

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mPCSK1

Plasmid

#122672

Purpose

Expresses mouse PCSK1 in mammalian cells

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

proprotein convertase subtilisin/kexin type 1 (PCSK1) (Pcsk1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pSens-4OmeNB

Plasmid

#216231

Purpose

Expresses GFP in response to 4'-O-Methylnorbelladine

Depositor

Andrew Ellington, Simon d'Oelsnitz

Article

d'Oelsnitz et al Nat Commun. 2024 Mar 7;15(1):2084. doi:

Inserts

RamR-4NB2
sfGFP

Use

Synthetic Biology

Tags

Expression

Mutation

K64T, L67M, C135D, S138G

Promoter

J23103 and Pramr

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pNb4OMT-A53M

Plasmid

#216232

Purpose

Expresses a mutant Norbelladine 4'-O-Methyltransferase

Depositor

Andrew Ellington, Simon d'Oelsnitz

Article

d'Oelsnitz et al Nat Commun. 2024 Mar 7;15(1):2084. doi:

Insert

Nb4OMT-A53M

Use

Synthetic Biology

Tags

Expression

Mutation

A53M

Promoter

P150

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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Pet15b-Internalization Control

Plasmid

#186553

Purpose

Negative Control mediating endosomal localization

Depositor

Jean Gariepy

Article

Mohammed et al J Control Release. 2012 Nov 28;164(1):58

Insert

10R-8His-EGFP

Use

Tags

8xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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Caspase-6

Plasmid

#100102

Purpose

Bacterial expression of Caspase-6

Depositor

Jim Wells

Article

Seaman et al Cell Death Differ. 2016 Oct;23(10):1717-

Insert

Caspase-6 (CASP6 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV HDM2(C464A)

Plasmid

#12086

Depositor

Tyler Jacks

Article

Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

Insert

HDM2 (MDM2 Human)

Use

Tags

Expression

Mammalian

Mutation

C464A: RING-finger-domain mutant

Promoter

Available since

June 8, 2006

Availability

Academic Institutions and Nonprofits only
pEGFP-N3-TPC2

Plasmid

#80153

Purpose

Intracellular Ca2+ store marker

Depositor

Santiago Di Pietro

Article

Ambrosio et al Mol Biol Cell. 2015 Sep 15;26(18):3263-7

Insert

TPCN2 (TPCN2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pExpreS2-1

Plasmid

#175360

Purpose

ZeoR backbone for recombinant protein overexpression with Drosophila ExpreS2 Platform

Depositor

ExpreS2ion Biotechnologies

Article

Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Fused Actin-HSP70

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL90002

Plasmid

#68261

Purpose

Level 0 Golden Gate Part: Promoter U6-26 (Arabidopsis thaliana)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter: U6-26 (Arabidopsis thaliana)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pV132-vCas9

Plasmid

#203223

Purpose

Mammalian expression of vCas9

Depositor

Phil Sharp, Vikash Chauhan, Robert Langer

Article

Chauhan et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

Insert

vCas9

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

S55R R976A K1003A T1314R

Promoter

CBh

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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