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Plasmid#195223PurposeMammalian expression vector containing GFP tagged Podocalyxin. PKC phosphorylation-deficient mutant.DepositorInsertPODXL (PODXL Rabbit)
UseTagsExpressionMammalianMutationS481APromoterAvailable sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-eGFP:HA-mTREK-1
Plasmid#224773PurposeMammalian expression vector for generating mouse TREK-1DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-eGFP:HA-mTREK-1(S131C)
Plasmid#224774PurposeMammalian expression vector for generating mouse TREK-1DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseTagsExpressionMammalianMutationS131CPromoterCMVAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1(S131C)
Plasmid#224744PurposeIn vitro transcription for Oocyte expression of mTREK-1(S131C)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationS131CPromoterT7Available sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1 (C159A/C219A)
Plasmid#224751PurposeIn vitro transcription for Oocyte expression of mTREK-1 (C159A/C219A)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationC159A/C219APromoterT7Available sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1(S131A)
Plasmid#224745PurposeIn vitro transcription for Oocyte expression of mTREK-1(S131A)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationS131APromoterT7Available sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1(G171F)
Plasmid#224747PurposeIn vitro transcription for Oocyte expression of mTREK-1(G171F)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationG171FPromoterT7Available sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1(S131C/K271Q)
Plasmid#224750PurposeIn vitro transcription for Oocyte expression of mTREK-1(S131C/K271Q)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationS131C / K271QPromoterT7Available sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1(A286F)
Plasmid#224746PurposeIn vitro transcription for Oocyte expression of mTREK-1(A286F)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationA286FPromoterT7Available sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1(tandem wt-S131C)
Plasmid#224759PurposeIn vitro transcription for Oocyte expression of mTREK-1(tandem wt-S131C)DepositorInsertTandem TREK-1 (wt-S131C) (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationS562CPromoterT7Available sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1(S131C/G171F)
Plasmid#224749PurposeIn vitro transcription for Oocyte expression of mTREK-1(S131C/G171F)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationS131C / G171FPromoterT7Available sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1(tandem S131C-wt)
Plasmid#224758PurposeIn vitro transcription for Oocyte expression of mTREK-1(tandem S131C-wt)DepositorInsertTandem TREK-1 (S131C-wt) (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationS131CPromoterT7Available sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1(S131C/A286F)
Plasmid#224748PurposeIn vitro transcription for Oocyte expression of mTREK-1(S131C/A286F)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationS131C / A286FPromoterT7Available sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1(G137I)
Plasmid#133272PurposeX. laevis expression vector. It will generate the mouse TREK-1 channelDepositorInsertPotassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
UseOocyte expressionTagsExpressionMutationG137IPromoterT7Available sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
Polymerase expression - pCMV-Phi29(+exo) (LM2759)
Plasmid#208964PurposeUnfused Phi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.DepositorInsertPhi29(+exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationPromoterCMV and T7Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCCR4_168-552-D412AN414A_R
Plasmid#147427PurposeInsect Expression of DmCCR4_168-552-D412AN414ADepositorInsertDmCCR4_168-552-D412AN414A (twin Fly)
UseTagsExpressionInsectMutation4 silent mutations and 1 non silent mutations com…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCCR4-D412AN414A_R
Plasmid#147428PurposeInsect Expression of DmCCR4-D412AN414ADepositorInsertDmCCR4-D412AN414A (twin Fly)
UseTagsExpressionInsectMutationThe following variations comprises from our S2 ce…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCCR4_168-552_I
Plasmid#146612PurposeInsect Expression of DmCCR4_168-552DepositorInsertDmCCR4_168-552 (twin Fly)
UseTagsExpressionInsectMutation4 silent mutations and 1 non silent mutations com…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCCR4_168-552_I
Plasmid#146603PurposeInsect Expression of DmCCR4_168-552DepositorInsertDmCCR4_168-552 (twin Fly)
UseTagsExpressionInsectMutation4 silent mutations and 1 non silent mutations com…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEE041
Plasmid#176798PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert410
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only