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Showing: 3881 - 3900 of 27972 results

  2. pExpreS2-1-CR-PA

    Plasmid
    #175445
    Purpose
    HiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A tagged proteins with ExpreS2 Platform
    Depositor
    Wyatt Yue
    Article
    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8
    Type
    Empty backbone
    Use
    Tags
    BiP Secretion Peptide and Thrombin-Protein A
    Expression
    Insect
    Mutation
    Promoter
    Fused Actin-HSP70
    Available since
    March 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    175445-plasmid-map-sequence-id-358137

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  8. pHL836

    Plasmid
    #62827
    Purpose
    Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::ipex::Asp terminator + PLtetO-1::RBS (st7) hfq::T1 terminator + ColE1
    Depositor
    Han Lim
    Article
    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv
    Inserts
    gfp
    ipex leader
    hfq leader
    Use
    Synthetic Biology
    Tags
    ompC leader and st7 RBS
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    Aug. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. pHL1954

    Plasmid
    #62831
    Purpose
    Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLlacO-1::RBS (st7) hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1
    Depositor
    Han Lim
    Article
    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv
    Inserts
    M8::ryhB leader
    hfq leader
    gfp
    Use
    Synthetic Biology
    Tags
    m9::sodB leader and st7 RBS
    Expression
    Bacterial
    Mutation
    Promoter
    pConNoHind, pLlacO-1, and pLtetO-1
    Available since
    Aug. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  10. pHL1969

    Plasmid
    #62832
    Purpose
    Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1
    Depositor
    Han Lim
    Article
    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv
    Inserts
    M8::ryhB leader
    T710 hfq leader
    gfp
    Use
    Synthetic Biology
    Tags
    m9::sodB leader
    Expression
    Bacterial
    Mutation
    Promoter
    pConNoHind, pLlacO-1, and pLtetO-1
    Available since
    Aug. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. pHL2107

    Plasmid
    #62841
    Purpose
    Lim Lab plasmid: AmpR + PconNoHindM8::micC::Asp terminator + PLlacO-1::T710::hfq::T1 terminator + PLtetO-1m9::ompC::gfp::T1T2 terminator + ColE1
    Depositor
    Han Lim
    Article
    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv
    Inserts
    m8::micC leader
    T710 hfq leader
    gfp
    Use
    Synthetic Biology
    Tags
    m9::ompC leader
    Expression
    Bacterial
    Mutation
    Promoter
    pConNoHind, pLlacO-1, and pLtetO-1
    Available since
    Aug. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. pcDNA3-PKAsub(T/A)-YPet-NES

    Plasmid
    #138205
    Purpose
    Encodes negative-control substrate fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKAsub(T/A)-YPet-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Availability
    Academic Institutions and Nonprofits only
    138205-plasmid-map-sequence-id-307407

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  13. pcDNA3.1-V5H6-mPCSK3 D153A

    Plasmid
    #122681
    Purpose
    Expresses mutated mouse PCSK3 in mammalian cells (aspartic acid at position 153 mutated to alanine via site directed mutagenesis)
    Depositor
    Liming Pei
    Article
    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:
    Insert
    Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    changed Aspartic Acid 153 to Alanine
    Promoter
    Available since
    May 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    122681-plasmid-map-sequence-id-244746

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  14. pMT-sox10-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80063
    Purpose
    Sox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Sox10 promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80063-plasmid-map-sequence-id-417621

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  16. A3Ai-Cas9n-UGI-NLS

    Plasmid
    #109425
    Purpose
    Expresses human APOBEC3A containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
    Depositor
    Reuben Harris
    Article
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Insert
    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (APOBEC3A Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Insertion of an L1 intron into the coding sequenc…
    Promoter
    CMV
    Available since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    109425-plasmid-map-sequence-id-212947

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  18. pMIG-hNFATc2/C - AVITEV

    Plasmid
    #74058
    Purpose
    retroviral expression plasmid for human NFATc2/C with C-terminal BirA-biotinylation signal and TEV celavage site
    Depositor
    Ria Baumgrass
    Article
    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.
    Insert
    human NFATc2, isoform C (NFATC2 Human)
    Use
    Retroviral
    Tags
    AVI-TEV
    Expression
    Mammalian
    Mutation
    silent mutation A1723C in the sequence of NM_1730…
    Promoter
    pMSCV-LTRs
    Available since
    June 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    74058-plasmid-map-sequence-id-142261

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  19. pαH-S-GSAS/D614G

    Plasmid
    #164566
    Purpose
    SARS-CoV-2 Spike protein with D614G mutation (S-GSAS-D614G variant)
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:
    Insert
    Spike (S-GSAS-D614G variant)
    Use
    Tags
    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Available since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    164566-plasmid-map-sequence-id-324830

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  20. pICE-HA-CtIP-siR-N289A-H290A

    Plasmid
    #82032
    Purpose
    Plasmid for constitutive or doxy-inducible expression of N289A+H290A mutant of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Sébastien Britton, Patrick Calsou
    Article
    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10
    Insert
    CtIP (RBBP8 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Endonuclease-dead mutant: N289A+H290A CtIP; Mutat…
    Promoter
    CMV-tet
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82032-plasmid-map-sequence-id-155382

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Showing: 3881 - 3900 of 27972 results