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Plasmid#138559PurposeExpresses human codon-optimized Sniper-SpCas9 and blasticidin resistance: EFS promoter-Sniper-Cas9-NLS-FLAG-P2A-BSDDepositorInsertSniper-Cas9
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationF539S, M763I, K890NPromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pODN-AAVS1-mNG-CAAX
Plasmid#183870PurposeRepair template for the stable expression of a mNeonGreen-CAAX fusion in human cells using CRISPR/Cas9.DepositorInsertAAVS1 homology arms with CMV-driven mNeonGreen-CAAX fusion (AAVS1 Human)
UseCRISPR; Donor templateTagsmNeonGreen-CAAXExpressionMammalianMutationHomology arms contain point mutations to remove t…PromoterCMVAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
QSOX1-luciferase
Plasmid#113352Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of QSOX1 geneDepositorInsertQSOX1-enhancer (QSOX1 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
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ATP9A-Tol2
Plasmid#113385Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of ATP9A geneDepositorInsertATP9A-enhancer (ATP9A Human)
UseTol2 reporterTagsExpressionMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
ATP9A-luciferase
Plasmid#113354Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of ATP9A geneDepositorInsertATP9A-enhancer (ATP9A Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag Jnk2a2(apf)
Plasmid#13761DepositorInsertJnk2a2(apf) (MAPK9 Human)
UseTagsFlagExpressionMammalianMutationreplaced dual phosporylation motif THr(183)-Pro-T…PromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-EMTB-TurboID-V5
Plasmid#190736PurposeMammalian expression of EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)DepositorInsertensconsin (MAP7 Human)
UseTagsMyc, TurboID, and V5ExpressionMammalianMutationMicrotubule binding-domain of ensconsin (amino ac…PromoterCMVAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD_Ub11TCA
Plasmid#174517PurposeRecoded ubiquitin reporter with TCA replacing codon 11 of wt ubiquitinDepositorInsertUbiquitin
UseTags6xHisExpressionBacterialMutationRecoded with TCA replacing codon 11 of wt ubiquit…PromoteraraBADAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
QSOX1-Tol2
Plasmid#113383Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of QSOX1 geneDepositorInsertQSOX1-enhancer (QSOX1 Human)
UseTol2 reporterTagsExpressionMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQCXIH BRGI-Flag
Plasmid#19148DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SNAP-CRY2-VHH(GFP)
Plasmid#58370PurposeFor use in light-induced inactivation of GFP fused protein through the interaction with CIB1-MPDepositorInsertCRY2[1-498] (CRY2 Mustard Weed)
UseTagsSNAP and vhhGFP4ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
G608G lamin A minigene
Plasmid#20292DepositorInsertlamin A (LMNA Human)
UseTagsGFPExpressionMammalianMutationG606G:GGC>GGT Mutation causes aberrant spli…PromoterAvailable sinceMarch 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJB556
Plasmid#115966PurposeSGP insert for replicon MoCloDepositorInsertLvl0 -98/15 SGP 2xTetApt-STOP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKD072
Plasmid#136472PurposeSSB-C-term-GFP fusion expression plasmidDepositorInsertSSB-C-term-GFP (ssb E. coli)
UseTagssuperfolder GFPExpressionBacterialMutationsuperfolder GFP attached to C-terminus at Phe 178PromoterT7 promoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK0_003
Plasmid#123925PurposeEncodes the spCas9 cassette and spCas9 gRNA GFP dropout expression cassette final destination vector as a type 0 part to be used in the MTK systemDepositorInsertCas9-sgRNA destination
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpeCas9-HF
Plasmid#192141PurposeExpresses highly specific SpeCas9, and cloning backbone for sgRNADepositorInsertSpeCas9-HF
UseAAVTagsExpressionMammalianMutationSpeCas9-HF (R247A, N415A, S421A)PromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-ECT2_sgRNA
Plasmid#183873PurposepX459V2.0-HypaCas9 plasmid with ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.DepositorInsertECT2 sgRNA spacer (ECT2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTol2-CreLite
Plasmid#131783PurposeTol2 destination vector carrying Ubi:CreLite with mTagBFP2 as a visual marker.DepositorInsertPhyBCreC, PIF6CreN, and mTagBFP2
UseCre/Lox and Synthetic Biology; Zebrafish tol2 tra…TagsmTagBFP2ExpressionMammalianMutationPromoterUbiAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-ACTB_sgRNA
Plasmid#183885PurposepX459V2.0-HypaCas9 plasmid with ACTB sgRNA for N-terminal tagging of beta-actin in human cells.DepositorInsertACTB sgRNA spacer (ACTB Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only