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Showing: 3861 - 3880 of 6891 results
  1. Sniper-Cas9

    Plasmid
    #138559
    Purpose
    Expresses human codon-optimized Sniper-SpCas9 and blasticidin resistance: EFS promoter-Sniper-Cas9-NLS-FLAG-P2A-BSD
    Depositor
    Insert
    Sniper-Cas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    F539S, M763I, K890N
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
  2. pODN-AAVS1-mNG-CAAX

    Plasmid
    #183870
    Purpose
    Repair template for the stable expression of a mNeonGreen-CAAX fusion in human cells using CRISPR/Cas9.
    Depositor
    Insert
    AAVS1 homology arms with CMV-driven mNeonGreen-CAAX fusion (AAVS1 Human)
    Use
    CRISPR; Donor template
    Tags
    mNeonGreen-CAAX
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Promoter
    CMV
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. pCDNA3-EMTB-TurboID-V5

    Plasmid
    #190736
    Purpose
    Mammalian expression of EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)
    Depositor
    Insert
    ensconsin (MAP7 Human)
    Use
    Tags
    Myc, TurboID, and V5
    Expression
    Mammalian
    Mutation
    Microtubule binding-domain of ensconsin (amino ac…
    Promoter
    CMV
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. pKD072

    Plasmid
    #136472
    Purpose
    SSB-C-term-GFP fusion expression plasmid
    Depositor
    Insert
    SSB-C-term-GFP (ssb E. coli)
    Use
    Tags
    superfolder GFP
    Expression
    Bacterial
    Mutation
    superfolder GFP attached to C-terminus at Phe 178
    Promoter
    T7 promoter
    Available since
    April 10, 2020
    Availability
    Academic Institutions and Nonprofits only
  5. MTK0_003

    Plasmid
    #123925
    Purpose
    Encodes the spCas9 cassette and spCas9 gRNA GFP dropout expression cassette final destination vector as a type 0 part to be used in the MTK system
    Depositor
    Insert
    Cas9-sgRNA destination
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
  6. pTol2-CreLite

    Plasmid
    #131783
    Purpose
    Tol2 destination vector carrying Ubi:CreLite with mTagBFP2 as a visual marker.
    Depositor
    Insert
    PhyBCreC, PIF6CreN, and mTagBFP2
    Use
    Cre/Lox and Synthetic Biology; Zebrafish tol2 tra…
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    Ubi
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 3861 - 3880 of 6891 results