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Plasmid#166102PurposeThis plasmid express a sgRNA that targets the IDH2 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH2-135 by homologous recombination.DepositorArticleInsertIDH2 sgRNA #7 (IDH2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pROS13_IDH1 #4
Plasmid#166101PurposeThis plasmid express a sgRNA that targets the IDH1 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH1-67 by homologous recombination.DepositorArticleInsertIDH1 sgRNA #4 (IDH1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJT174_GalL_A3A-Y130F-BE3
Plasmid#145123PurposeExpressing base editor A3A-Y130F-BE3 in yeast cellsDepositorInsertA3A(Y130F)-BE3
UseCRISPRTagsExpressionYeastMutationA3A(Y130F); spCas9(D10A)PromoterGalLAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT173_GalL_A3A-R128A-BE3
Plasmid#145122PurposeExpressing base editor A3A-R128A-BE3 in yeast cellsDepositorInsertA3A(R128A)-BE3
UseCRISPRTagsExpressionYeastMutationA3A(R128A); spCas9(D10A)PromoterGalLAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT113_GalL_cCDA1-xBE3
Plasmid#145079PurposeExpressing base editor cCDA1-xBE3 in yeast cellsDepositorInsertcCDA1-xBE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-HXT1-FAS1up
Plasmid#126746Purposevector for replacing the promoter of FAS1 coding geneDepositorInsertfas1 upstream
UseTags6xHisExpressionYeastMutationPromoterHXT1Available sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNIA-CEN-FLAG-LINXa4-Bre1
Plasmid#73617PurposeControl of the E3 ligase Bre1 with LINXa in yeast.DepositorInsertLINXa4-Bre1dNLS
UseSynthetic BiologyTagsFLAGExpressionYeastMutationBre1 point mutations K8A, K9A and K11APromoterADH1Available sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTH394-SUP45-L34S
Plasmid#29373DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
UseTagsExpressionYeastMutationL34S mutant gene (corresponding to the sup45-36ts…PromoterAvailable sinceMay 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTH401-SUP45-R65C
Plasmid#29381DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
UseTagsExpressionYeastMutationR65C mutant gene (corresponding to the sup45-3 al…PromoterAvailable sinceMay 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAV10
Plasmid#63213PurposepUC19 backbone (Amp), contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. NotI or FseI sites for TU digest.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA
Plasmid#73796PurposepRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNADepositorInsertsdCas9-Mxi1
Tet Repressor
Structural gRNA for S pyogenes
UseCRISPRTagsMxi1 and NLSExpressionYeastMutationD10A, H840APromoterpGPM1, pRPR1(TetO), and pTef1Available sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS426-HsFECH
Plasmid#174524PurposeExpresses human ferrochelatase (FECH) in yeastDepositorInsertFECH with yeast HEM15 promoter and terminator (FECH Synthetic, Human, Budding Yeast)
UseTagsExpressionYeastMutationLacks original mitochondrial targeting sequence (…PromoterHEM15 (yeast, 257 bp)Available sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH2971
Plasmid#100955PurposeCas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. KANMX markerDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pJH2972
Plasmid#100956PurposeCas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. URA3MX markerDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS403-HIS3-pINO4-SV40NLS-PfV-GS-Sapphire
Plasmid#203658PurposeYeast integrative vector for the expression of the Sapphire-tagged nuclear localizing 40nm GEMs under the HIS3 promoter.DepositorArticleInsertPfV
UseTagsSapphireExpressionYeastMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJH2970
Plasmid#100954PurposeCas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HIS3MX markerDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCORE-UK
Plasmid#72238PurposeContains the CORE cassette with convergent KlURA3 and KanMX4 markersDepositorInsertsKlURA3
kanMX4
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS305-LEU2-pINO4-SV40NLS-PfV-GS-Sapphire
Plasmid#203655PurposeYeast integrative vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the INO4 promoter.DepositorArticleInsertPfV
UseTagsSapphireExpressionYeastMutationPromoterAvailable sinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only