Addgene

We narrowed to 3,954 results for: chm

Showing: 3821 - 3840 of 3954 results

Results per page:

Sort results by:

p3xFLAG-ATF6

Plasmid

#11975

Purpose

For mammalian expression of human ATF6 with a 3x FLAG tag

Depositor

Ron Prywes

Article

Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

Insert

ATF6 (ATF6 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-Sp-gRNA-CCR5_DF_B584b_attB

Plasmid

#182148

Purpose

To insert attB attachment site at CCR5 in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

CCR5-B584b-attB pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-Sp-gRNA-CCR5_DF_A509b_attB

Plasmid

#182147

Purpose

To insert attB attachment site at CCR5 in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

CCR5-A509b-attB pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-Sp-gRNA-AAVS1_B3903c_attP

Plasmid

#182144

Purpose

To insert attP attachment site at AAVS1 in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

AAVS1_B3903c_attP pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-Sp-gRNA-AAVS1_A3786c_attP

Plasmid

#182143

Purpose

To insert attP attachment site at AAVS1 in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

A3786c-attP pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS4134

Plasmid

#131747

Purpose

Human reduced folate transporter 1 SLC19A1 for lentiviral transduction, copGFP transduction marker

Depositor

Christina Smolke

Article

Xiang et al Nat Commun. 2019 Sep 23;10(1):4327. doi:

Insert

Human reduced folate carrier 1 (SLC19A1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-Sp-gRNA-ALB_DF_B358c_attB

Plasmid

#182146

Purpose

To insert attB attachment site at ALB in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

ALB_B358c_attB pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-Sp-gRNA-ALB_DF_A277c_attB

Plasmid

#182145

Purpose

To insert attB attachment site at ALB in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

Alb_A277c_attB pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS3Clux

Plasmid

#55172

Purpose

lux-reporter vector, integration at sacA, ampr, cmr

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Tags

Expression

Mutation

Promoter

none

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-mas-KB-1753-Nluc

Plasmid

#158603

Purpose

Plasmid expressing KB-1753-Nluc in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 - linker (GGTGGS) - Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-U6-sgRNA-PGK- mRFP-T2A-PuroR

Plasmid

#194925

Purpose

mRFP and T2A linker are inserted in between the hPGK promoter and the puromycin resistance gene (PuroR) on pGL3-U6-sgRNA-PGK-puromycin to allow simultaneous monitoring and enrichment of transfected ho

Depositor

Hao Xu

Article

Ayo et al J Genome Ed Regul. 2022;2:246146. doi: 1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

hPGK

Available since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-mas-GRK2RH-Nluc

Plasmid

#158605

Purpose

Plasmid expressing GRK2(RH)-Nluc in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - GRK2 RH domain - linker (GGTGGS) - Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-MTS-WT-c-Src-FLAG

Plasmid

#44652

Depositor

Yoshimi Homma

Article

Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

Insert

v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)

Use

Tags

FLAG tag and mitochondria-targeting sequence (res…

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

April 26, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR sgSLC19A1

Plasmid

#102314

Purpose

genetic depletion of SLC19A1

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgSLC19A1 (SLC19A1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-Sp-gRNA-IDS2_DF_A4b_attP_fwd

Plasmid

#182149

Purpose

To insert attP-fwd attachment site at IDS2 in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

IDS2-A4b-attP-fwd pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-Sp-gRNA-IDS_DF_D1b_attB_rev

Plasmid

#182152

Purpose

To insert attB-rev attachment site at IDS in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

IDS-D1b-attB-rev pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-Sp-gRNA-IDS_DF_C2c_attB_rev

Plasmid

#182151

Purpose

To insert attB-rev attachment site at IDS in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

IDS-C2c-attBrev pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-Sp-gRNA-IDS2_DF_B7b_attP_fwd

Plasmid

#182150

Purpose

To install attP-fwd attachment site at IDS2 in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

IDS2-B7b-attP-fwd pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-hygro-hPGAP3-3HA

Plasmid

#50373

Purpose

Express C-terminally triple HA tagged human PGAP3 in mammmalian cells

Depositor

Reika Watanabe

Article

Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

Insert

PGAP3 post-GPI attachment to proteins 3 (PGAP3 Human)

Use

Tags

3x HA

Expression

Mammalian

Mutation

Promoter

SR alpha

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
pA-RFP-rG2

Plasmid

#188969

Purpose

IPTG inducible mCherry with sgRNA

Depositor

Seung-Goo Lee

Article

Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

Inserts

mCherry
sgRNA: agtccatgtaatcagcgtctactagt

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Ptrc

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge