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Plasmid#145746PurposeBacterial Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMINTC3GH (Kan)
Plasmid#110076PurposeAnalogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPO635
Plasmid#195297PurposepACYC-plac-MycaTnsABC, lac promoter expressing transposase genes TnsABC of McCAST (Tn7575).DepositorInsertTnsABC operon of McCAST (Tn7575)
UseTagsExpressionBacterialMutationPromoterIPTG inducible lac promoterAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-SCP1-ccdB
Plasmid#131628PurposeModified STARR-seq plasmid to measure enhancer activity (SCP1 promoter; ccdb gene at cloning site)DepositorTypeEmpty backboneUseLuciferaseTagsExpressionBacterial and MammalianMutationPromoterSCP1Available sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_RPS10_K138/139R
Plasmid#127138DepositorInsertRPS10 (RPS10 Human)
UseLentiviralTagsExpressionMutationK138R/K139RPromoterAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB_init_Sb92
Plasmid#159424PurposeExpression of the anti-TGP (thermostable GFP) synthetic nanobody Sb92DepositorInsertSb92
UseTagsc-Myc and poly-HisExpressionBacterialMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL4-Gateway-SCP1
Plasmid#71510PurposehumanSTARR-seq validation vector with Gateway cassette upstream of the firefly luciferase gene and the SCP1 core promoterDepositorTypeEmpty backboneUseHumanstarr-seq validation vectorTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
CDS12_mTurquoise2
Plasmid#136089PurposeFP for N-term fusion with a CTAGDepositorInsertCDS12_mTurquoise2(noStop)
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
CDS_LUC
Plasmid#136083PurposeFirefly luciferaseDepositorInsertCDS_LUC
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
p15aC-1D-5
Plasmid#107865PurposeBacterial expression of PIS and PI4P5KDepositorInsertsPIS
phosphatidylinositol 4-phosphate 5-kinase
UseTagsmycExpressionBacterialMutationPromoterproD and proD (in operon)Available sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWS1837
Plasmid#123058Purpose_-factor pheromoneDepositorInsert_-factor [3-4a]
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pdestMB14
Plasmid#26415DepositorInsertattR4-ccdB-attR2
UseTagsExpressionBacterial and WormMutationPromoterAvailable sinceApril 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGL2_174
Plasmid#199106PurposeLevel 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporterDepositorInsertpNG2 ORI plus NGS barcode 22, gentamicin resistance cassette, mScarlet-I cassette, oriT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL2_169
Plasmid#199101PurposeLevel 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporterDepositorInsertpIJ101 ORI plus NGS barcode 15, gentamicin resistance cassette, mScarlet-I cassette, oriT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046764
Plasmid#145673PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;StrepIIExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBTK150
Plasmid#183126PurposeBTK Type 2 promoter part for golden gate assemblyDepositorInsertReverse rnpB-T1 terminator and forward CP25 promoter for dsRNA expression
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL2_177
Plasmid#199109PurposeLevel 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporterDepositorInsertpBC1 ORI plus NGS barcode 25, gentamicin resistance cassette, mScarlet-I cassette, oriT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKEB31
Plasmid#31224DepositorInsertccdB and Cm resistance gene
UseTagsExpressionMutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046959
Plasmid#145722PurposeBacterial Expression plasmid for SARS-CoV-2 ORF3a proteinDepositorInsertSARS-CoV-2 ORF3a protein (ORF3a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJB089
Plasmid#98914PurposeExpress Dronpa tagged ZapA and pAmCherry1 tagged FtsZ in E coliDepositorInsertsFtsZ
ZapA
UseTagsDronpa and pAmCherry1ExpressionBacterialMutationPromoterT5-lacAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only