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Plasmid#87342PurposeTo express human Nup35 in mammalian cellsDepositorInsertnucleoporin 35 (NUP35 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-RosaR4 KKR mutations
Plasmid#37199DepositorInsertFokI (fused to RosaR4 ZFN)
UseZfn expressionTagsHA, ROSA26 eZFNs R4, and SV40 NLSExpressionMammalianMutationI538K, E490K, and H537RPromoterCMVAvailable sinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pOCC5
Plasmid#118863Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C proteaseDepositorInsertNcoI-HIS6-3C-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
8xGliBS-IVS2-mCherry-NLS-Odc1-polyA-Tol2
Plasmid#84603PurposeTol2-based hedgehog signal reporter plasmid for zebrafish transgenesisDepositorInsertmCherry
UseZebrafish transgenesisTagsODC1 destabilizing elementExpressionMutationPromoter8xGliBS-delta-crystallin minimal promoterAvailable sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-TetO(3G)-WPRE
Plasmid#186206PurposetTA dependent gene expression vectorDepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterTetO(3G)Available sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMVTNT-T7-KPNA3
Plasmid#26679DepositorInsertKaryopherin Alpha 3 (KPNA3 Human)
UseTagsT7 (MASMTGGQQMG)ExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHR-PGK-ABI-dCas9-P2A-mcherry
Plasmid#121513Purposeexpression of AB1-fused-dcas9 plasmidsDepositorInsertdCas9
UseLentiviralTagsAB1 and P2A-mcherryExpressionMammalianMutationPromoterPGKAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRG645_lexO-AscI_LexA-TF_LEU2MX
Plasmid#154813PurposeInducible expression of AscI endonuclease in S. cerevisiaeDepositorInsertsAscI endonuclease
LexA-ER-B112
UseTagsFLAG and NLSExpressionYeastMutationPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cyto-CaNAR2
Plasmid#64729PurposeCytosol-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.DepositorInsertCaNAR2-NES
UseTagsCerulean3, Nuclear export signal (NES), and YPetExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/V5-His Snk/Plk2
Plasmid#16015DepositorInsertSnk/Plk2 (PLK2 Human)
UseTagsV5-HisExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-LBR-EGFP-polyA
Plasmid#59748PurposeExpress LBR-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.DepositorInsertLBR-EGFP
UseTagsEGFPExpressionMammalianMutationPromoterCMV and T7Available sinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
Stx1a-12aa-mCer
Plasmid#53236PurposeFluorescent probe: Syntaxin 1A, C-terminally-tagged with monomeric Cerulean, connected with a 12 amino acid linker. Expressed in mammalian cells, used with SNAP25B-12aa-mCit to measure FRET & dispers.DepositorInsertSyntaxin 1A (Stx1a Rat)
UseTags12aa-linker and mCeruleanExpressionMammalianMutationPromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG roGFP2-mito
Plasmid#168509PurposeExpression of mito-roGFP2 (mitochondrial matrix targeted roGFP2) used for monitoring dynamcs of mitochondrial Redox potentialDepositorInsertmito-roGFP2
UseTagsroGFP2ExpressionMammalianMutationPromoterCAGAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc460
Plasmid#161940PurposeCarries Golden Gate substitution insert for IBM with gp41-1 intein. PPhlF as C-lobe promoterDepositorInsertpSB1A3-BbsI-GY-gp-41-1N-cat-PPhlF-RiboJ-B32-gp41-1C-SS-SapI
UseTagsExpressionBacterialMutationPromoterPPhlFAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-flag-mZscan4d-polyA
Plasmid#61831PurposeFor mRNA synthesis or expression in mammalian cellsDepositorInsertZscan4d (Zscan4d Mouse)
UseTagsflagExpressionMammalianMutationPromoterCMV and T7Available sinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-LBR-mRFP1-polyA
Plasmid#59749PurposeExpress LBR-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.DepositorInsertLBR-mRFP1
UseTagsmRFP1ExpressionMammalianMutationaa 1-249 of LBRPromoterCMV and T7Available sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOCC7
Plasmid#118876Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_BIRC6
Plasmid#197974PurposeEntry clone for wt BIRC6DepositorInsertBIRC6 (BIRC6 Human)
UseOtherTagsExpressionMutationL1332VPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-3xFLAG-WPRE
Plasmid#127862PurposepAAV plasmid expressing 3xFLAG under the hSyn promoterDepositorInsert3xFLAG
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only