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Plasmid#198887PurposeExpress A3Bctd-D314E BE3-based base editorDepositorInsertApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
UseTagsNLSExpressionMammalianMutationD314EPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
eA3Ai-Cas9n-UGI-NLS
Plasmid#207165PurposeExpress A3A with an N57G point mutation and an intron in a BE3-based base editorDepositorInsertApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3A (APOBEC3A Human)
UseTagsCas9-UGI--NLSExpressionMammalianMutationN57GPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
eA3Ai-max
Plasmid#207166PurposeExpress A3A with an N57G point mutation and an intron in a BE4max-based base editorDepositorInsertApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3A (APOBEC3A Human)
UseTagsCas9-UGI-UGI-NLS and NLSExpressionMammalianMutationN57GPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCCR4_1-166_H
Plasmid#146524PurposeInsect Expression of DmCCR4_1-166DepositorInsertDmCCR4_1-166 (twin Fly)
UseTagsExpressionInsectMutation1 silent mutation compared to the sequence given …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCCR4_1-166_H
Plasmid#146525PurposeInsect Expression of DmCCR4_1-166DepositorInsertDmCCR4_1-166 (twin Fly)
UseTagsExpressionInsectMutation1 silent mutation compared to the sequence given …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
DsRed -7e
Plasmid#191765PurposeExpression of DsRed fused to a negatively charged polypeptide. Overall charge on tetramer: -7eDepositorInsertDsRed
UseTagsN terminal fusion of negatively charged polypetod…ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBABR mCherry-Bcd-LEXY
Plasmid#182596PurposeDrosophila integration plasmid expressing mCherry-Bcd-LEXY fusion protein under the maternal tubulin promoter.DepositorInsertBcd (bcd Fly)
UseTagsLEXY and mCherryExpressionInsectMutationPromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBABR Bcd-LEXY
Plasmid#182594PurposeDrosophila integration plasmid expressing Bcd-LEXY fusion protein under the maternal tubulin promoter.DepositorInsertBicoid (bcd Fly)
UseTagsLEXYExpressionInsectMutationPromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCCR4_A
Plasmid#145894PurposeInsect Expression of DmCCR4DepositorInsertDmCCR4 (twin Fly)
UseTagsExpressionInsectMutationThe following variations comprises from our S2 ce…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
Bmpr2 gRNA#2
Plasmid#163389PurposeCas9-mediated knockout of Bmpr2 in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Bmpr2 gRNA#1
Plasmid#163388PurposeCas9-mediated knockout of Bmpr2 in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Bmpr2 gRNA#3
Plasmid#163390PurposeCas9-mediated knockout of Bmpr2 in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ash1-5p
Plasmid#169712PurposeBacterial Expression of truncated yeast Ash1 including encoding amino acids 420-480 with phosphorylation sites 429 and 470 removedDepositorInsertAsh1 (ASH1 Budding Yeast)
UseTags6xHisExpressionBacterialMutationTruncation including encoding amino acids 420-480…PromoterT7Available sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Stk11)-PGKpuroBFP-W
Plasmid#105043PurposeLentiviral gRNA plasmid targeting mouse Stk11 , co-expression of TagBFPDepositorInsertStk11 (Stk11 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Zfp219-g1)-PGKpuroBFP-W
Plasmid#105021PurposeLentiviral gRNA plasmid targeting mouse Zfp219 , co-expression of TagBFPDepositorInsertZfp219 (Zfp219 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Ndufa2-g1)-PGKpuroBFP-W
Plasmid#105030PurposeLentiviral gRNA plasmid targeting mouse Ndufa2 , co-expression of TagBFPDepositorInsertNdufa2 (Ndufa2 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Ndufa2-g2)-PGKpuroBFP-W
Plasmid#105031PurposeLentiviral gRNA plasmid targeting mouse Ndufa2 , co-expression of TagBFPDepositorInsertNdufa2 (Ndufa2 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Vps25-g1)-PGKpuroBFP-W
Plasmid#105033PurposeLentiviral gRNA plasmid targeting mouse Vps25 , co-expression of TagBFPDepositorInsertVps25 (Vps25 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Tcf7l1-g2)-PGKpuroBFP-W
Plasmid#105016PurposeLentiviral gRNA plasmid targeting mouse Tcf7l1 , co-expression of TagBFPDepositorInsertTcf7l1 (Tcf7l1 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX304 PCSK5 (R511C)-V5 blast
Plasmid#83104PurposeLentiviral vector for constitutive expression of human mutant PC5A (R511C) with C-terminal V5 tagDepositorInsertPCSK5 (PCSK5 Human)
UseLentiviralTagsV5ExpressionMammalianMutationChanged Arginine 511 to CysteinePromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only