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Plasmid#98677PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase II with N2ADepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
UseTags6xHisExpressionBacterialMutationN1775A, N1782APromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
RNAPII_CTD27-52_del1961-1970
Plasmid#98680PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase II with del1961-1970DepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
UseTags6xHisExpressionBacterialMutationdel1961-1970PromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
RNAPII_CTD27-52_N2A_S1966C
Plasmid#98682PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase II with N2A and S1966CDepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
UseTags6xHisExpressionBacterialMutationN1775A, N1782A, S1966CPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAW8ENdeI-cHAIAA7-spo5DSR
Plasmid#49873Purposefor Cre-lox cassette exchange of C-terminal HA-auxin-inducible degron tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTagsIAA17 degron tagExpressionMutationPromoterAvailable sinceJan. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-HSD17B13-WT-FLAGC
Plasmid#184501PurposeExpresses human HSD17B13 with a C-terminal FLAG-GFP tag. Confers G418 resistance.DepositorInsert17-beta-hydroxysteroid dehydrogenase 13 (HSD17B13 Human)
UseTagsFLAGC-GFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-HPGD-FLAGC
Plasmid#161915PurposeExpresses human HPGD with a C-terminal FLAG-GFP tag. Confers G418 resistance.DepositorInsertHPGD (HPGD Human)
UseTagsFLAG-GFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
FKBP-AP180-GFP
Plasmid#186577PurposeExpression of the fusion protein FKBP-AP180-GFP. Uses rat AP180 residues 328-896.DepositorInsertAP180 (Snap91 Rat)
UseTagsFKBP N-terminal GFP C-terminalExpressionMammalianMutationPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-GFPγ-SpHis5
Plasmid#60781PurposeYeast C-terminal tagging vector, which creates gene fusions with GFPγ using SpHis5 selectionDepositorInsertGFPγ
UseYeast integratingTagsExpressionMutationT65C S72A V163A I167T L231HPromoterAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
EC71171
Plasmid#154069PurposeLevel 0 Golden Gate vector, CRE with U5 intron at 254bp, in pICH41308 backboneDepositorInsertCRE_U5intron
UseSynthetic BiologyTagsExpressionMutationAll BsaI, Esp3I and BPiI restriction sites were r…PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub-YPet-NES
Plasmid#84635PurposeEncodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub-YPet-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationPromoterCMVAvailable sinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
EC71139
Plasmid#154062PurposeLevel 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Barley Construct)DepositorInsertpZmUbi
UseSynthetic BiologyTagsExpressionMutationAll BsaI, Esp3I and BpiI restriction sites were r…PromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Hyg-FHIT
Plasmid#16410DepositorInsertFHIT Hyg targeting (FHIT Human)
UseAAV; Adeno-associated viralTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET28 His6-mEGFP-D4H
Plasmid#226373PurposeProduction of recombinant mEGFP-D4H, a fluorescent probe binding cholesterolDepositorInsertD4H (pfoA Clostridium perfringens)
UseTagsHis6 and monomeric EGFPExpressionBacterialMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Enterovirus A71 (EV-A71) isolate 4643-TW98 3C protease with non-cleavable C-terminal His AviTag
Plasmid#228635PurposeBacterial expression of the A71 3C protease for assays that require immobilization using biotin-streptavidin interactionDepositorInsertEnterovirus A71 3C protease isoform A
UseTagsHis-AviExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
Enterovirus A71 (EV-A71) isolate 4643-TW98 2A protease with non-cleavable C-terminal His AviTag
Plasmid#228634PurposeBacterial expression of the A71 2A protease for assays that require immobilization using biotin-streptavidin interactionDepositorInsertEnterovirus Coxsackievirus A71 2A protease
UseTagsHis-AviExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_CRBN:1-442
Plasmid#211067PurposeMammalian expression of full-length human CRBN the C-terminal NanoLuc luciferase tag.DepositorInsertCRBN:M1-L442 (CRBN Human)
UseTagsNanoLuc luciferaseExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_DCAF1:1-1507
Plasmid#210950PurposeMammalian expression of full-length human DCAF1. C-terminal NanoLuc luciferase tag.DepositorInsertDCAF1:M1-E1507 (DCAF1 Human)
UseTagsNanoLuc luciferaseExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_CCNE1:1-410
Plasmid#211023PurposeMammalian expression of full-length human CCNE1. C-terminal NanoLuc luciferase tag.DepositorInsertCCNE1:M1-A410 (CCNE1 Human)
UseTagsNanoLuc luciferaseExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_FBXL2:1-423
Plasmid#211078PurposeMammalian expression of full-length human FBXL2. C-terminal NanoLuc luciferase tag.DepositorInsertFBXL2:M1-L423 (FBXL2 Human)
UseTagsNanoLuc luciferaseExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_RAB7A:1-207
Plasmid#211098PurposeMammalian expression of full-length human RAB7A. C-terminal NanoLuc luciferase tag.DepositorInsertRAB7A:M1-C207 (RAB7A Human)
UseTagsNanoLuc luciferaseExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only