Addgene

We narrowed to 10,091 results for: 11

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pBSc + MShab

Plasmid

#16146

Depositor

Larry Salkoff

Article

Pak et al J Neurosci. 1991 Mar . 11(3):869-80.

Insert

Mus musculus potassium voltage gated channel (Kcnb1 Fly, Mouse)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

N/A

Promoter

Available since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pCMV5 Flag-Smad1 (4SP/AP)

Plasmid

#14043

Depositor

Joan Massague

Article

Kretzschmar et al Genes Dev. 1997 Apr 15. 11(8):984-95.

Insert

Smad1 (4SP/AP) (SMAD1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Four Serine to Alanine mutations in the linker re…

Promoter

Available since

May 10, 2007

Availability

Academic Institutions and Nonprofits only
pReporter_3

Plasmid

#60564

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_3

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pJFRC210-10XUAS-FRT>STOP>FRT-myr::smGFP-OLLAS

Plasmid

#63170

Purpose

GAL4-responsive UAS “flp-On” spaghetti monster GFP-OLLAS reporter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"spaghetti monster GFP-OLLAS"

Use

Tags

N-myristoylation

Expression

Insect

Mutation

Promoter

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pReporter_12

Plasmid

#60572

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_12

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/M87 S245X

Plasmid

#92368

Purpose

expression of M87 S245X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

1-460 bp deleted, S245X

Promoter

CMV

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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dTomato_LD 0_CS G_tTA-BFP

Plasmid

#58871

Purpose

MESA target chain with dTomato ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA target chain with dTomato ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence

Use

AAV

Tags

EBFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 3, 2014

Availability

Academic Institutions and Nonprofits only

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aq-CFP

Plasmid

#36073

Depositor

Narasimhan Gautam

Article

Chisari et al PLoS One. 2009 Nov 11;4(11):e7797.

Insert

G protein subunit alpha q (Gnaq Mouse)

Use

Tags

CFP

Expression

Mammalian

Mutation

CFP inserted between amino acid 124 and 125

Promoter

CMV

Available since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-RhoA K6,7R

Plasmid

#11751

Depositor

Jeff Wrana

Article

Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

Insert

RhoA (RHOA Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

K6,7R

Promoter

Available since

Aug. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
pFosill-2

Plasmid

#89696

Purpose

to make Fosmids that can be converted into Illumina sequencable libraries

Depositor

Andreas Gnirke

Article

Williams et al Genome Res. 2012 Nov;22(11):2241-9. doi:

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pReporter_9

Plasmid

#60569

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_9

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pReporter_4

Plasmid

#60565

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_4

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pEBG-TFII-I N90

Plasmid

#22186

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2001 Mar 16. 276(11):8377-8

Insert

TFII-I (GTF2I Human)

Use

Tags

GST

Expression

Mammalian

Mutation

Contains the first 90 aa of TFII-I

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pReporter_13_switchBP

Plasmid

#62848

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp). attP upstream of attB in this plasmid.

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_13_switchBP

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pCFJ496

Plasmid

#44488

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

500bp Mos1, unc-119, fosmid cassette

Use

Fosmid recombineering cassette

Tags

Expression

Mutation

Promoter

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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mCherry_LD 0_CS G_tTA-BFP

Plasmid

#58856

Purpose

MESA target chain with mCherry ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA target chain with mCherry ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence

Use

AAV

Tags

EBFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pLpC-L-IFP-F[1] (mammalian)

Plasmid

#52901

Purpose

Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[1] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[1]

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Unknown

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pLpC-L-IFP-F[2] (mammalian)

Plasmid

#52902

Purpose

Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[2] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[2]

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Unknown

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pReporter_11

Plasmid

#60571

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_11

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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459 pME-etv2

Plasmid

#49002

Purpose

full length coding sequence for zebrafish etv2 gene in gateway middle entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

ets variant gene 2 (etsrp Zebrafish)

Use

Gateway middle entry

Tags

Expression

Mutation

Promoter

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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