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Plasmid#111605PurposeExpress human 33-G-X5 in 293T cellsDepositorInserthuman LRRC33 and GARP chimeria (NRROS Human)
UseTagsFlag tagExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRP_8xgRNA-FLuc
Plasmid#135937PurposeFirefly luciferase reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation.DepositorInsert8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')
UseLuciferaseTagsExpressionMammalianMutationPromoterminCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
SPOP-V5 (BTB)
Plasmid#169721PurposeMammalian Expression of mouse SPOP-BTB (L186D, L190D, L193D, I217K)DepositorInsertSPOP (Spop Mouse)
UseTagsV5ExpressionMammalianMutationL186D, L190D, L193D, I217KPromoterCMVAvailable sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCINeoFlag ALIX; I318A
Plasmid#89867PurposeExpresses full length Flag-tagged ALIX with mutation I318A in mammalian cells; internal ID WISP11-403DepositorInsertALIX (PDCD6IP Human)
UseTagsFlagExpressionMammalianMutationMutated isoleucine 318 to alaninePromoterCMVAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-hDF C2B
Plasmid#72583Purposehuman dysferlin C2B domain tagged with YFPDepositorInsertdysferlin C2B domain (DYSF Human)
UseTagsEYFPExpressionMammalianMutationC2B domainPromoterCMVAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-hDF-C2A
Plasmid#72582Purposehuman dysferlin C2A domain tagged with YFPDepositorInsertdysferlin C2A domain (DYSF Human)
UseTagsEYFPExpressionMammalianMutationC2A domainPromoterCMVAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-CTRLg2 (CG510)
Plasmid#139456PurposeLentiviral vector with non-targeting gRNA and puromycin selectable markerDepositorInsertNon-targeting sgRNA inserted; resistance gene: puroR
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
r-22. pT7-7-SNCA-9Asn
Plasmid#107297PurposePlasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 22 aa C-terminal moiety, which consists of 9 Asn and 13 GlyDepositorInsertSNCA-9Asn (SNCA Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
t2. pT7-7-SNCA-5Lys
Plasmid#107294PurposePlasmid for the expression of the construct SNCA-5Lys. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Lys and 7 GlyDepositorInsertSNCA-5Lys (SNCA Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
r-6. pT7-7-SNCA-2Asn
Plasmid#107296PurposePlasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 6 aa C-terminal moiety, which consists of 2 Asn and 4 GlyDepositorInsertSNCA-2Asn (SNCA Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
r. pT7-7-SNCA-5Asn
Plasmid#107295PurposePlasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Asn and 7 GlyDepositorInsertSNCA-5Asn (SNCA Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
s2. pT7-7-SNCA-5Asp
Plasmid#107293PurposePlasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Asp and 7 GlyDepositorInsertSNCA-5Asp (SNCA Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
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GFP-PODXL S481D
Plasmid#195224PurposeMammalian expression vector containing GFP tagged Podocalyxin. PKC phosphomimetic mutant.DepositorInsertPODXL (PODXL Rabbit)
UseTagsExpressionMammalianMutationS481DPromoterAvailable sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1
Plasmid#224743PurposeIn vitro transcription for Oocyte expression of mTREK-1DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationPromoterT7Available sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1(tandem wt-wt)
Plasmid#224757PurposeIn vitro transcription for Oocyte expression of mTREK-1(tandem wt-wt)DepositorInsertTandem TREK-1 (wt-wt) (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationPromoterT7Available sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
SynNotch TMD/Gal4-VP64 ICD (MTK 3b) (pAN1031)
Plasmid#194222PurposeMammalian Toolkit part 3b encoding SynNotch and GAL4-VP64 to be used in designing modular receptorsDepositorInsertSynNotch-GAL4 DBD-VP64 AD
UseSynthetic BiologyTagsExpressionMutationPromoterNoneAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET mNG-GECO1
Plasmid#201933PurposeExpresses mNG-GECO1 indicator in bacteriaDepositorInsertmNeonGreenGECO1
UseTagsHIS tagExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
A3Bctd-L7G-Cas9n-UGI-NLS
Plasmid#198886PurposeExpress A3Bctd-L7G BE3-based base editorDepositorInsertApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
UseTagsNLSExpressionMammalianMutationLoop 7 of A3GPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only