Addgene

We narrowed to 9,017 results for: yeast

Showing: 3781 - 3800 of 9017 results

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pJK1

Plasmid

#111722

Purpose

to express human BLM protein in yeast cells

Depositor

Ian Hickson

Article

Karow et al J Biol Chem. 1997 Dec 5;272(49):30611-4.

Insert

BLM (BLM Human)

Use

Tags

hexahistidine

Expression

Yeast

Mutation

a C-terminal hexahistidine tag was added, a yeast…

Promoter

GAL1

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pMK198 (GAL-OsTIR1)

Plasmid

#140655

Purpose

GAL-OsTIR1(WT)

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(WT)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt1

Plasmid

#39281

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Expression

Mutation

-1163 to +6 of nmt1 locus with a 4bp deletion (TA…

Promoter

Available since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only
pFA6a-kanMX6-P81nmt1

Plasmid

#39282

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Expression

Mutation

-1163 to +6 of nmt1 locus with a 7bp deletion (AT…

Promoter

Available since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only

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3410 pBM272 Bcl-XL

Plasmid

#8772

Depositor

Stanley Korsmeyer

Article

Gross et al Mol Cell Biol 2000 May;20(9):3125-36.

Insert

Bcl-XL (Bcl2l1 Mouse)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pFA6a-kanMX6-P3nmt1-3HA

Plasmid

#39283

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Expression

Mutation

-1163 to +6 of nmt1 locus

Promoter

Available since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pDRf1-4CL5-DsRed (JBEI-15461)

Plasmid

#87935

Purpose

Cloning vector for co-expressing a gene with 4CL5 in S.cerevisiae

Depositor

Dominique Loque

Article

Eudes et al Microb Cell Fact. 2016 Nov 21;15(1):198.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pRS303 Gal TDP-43 YFP

Plasmid

#27468

Depositor

Aaron Gitler

Article

Johnson et al Proc Natl Acad Sci U S A. 2008 Apr 29. 1

Insert

TDP-43 (TARDBP Human)

Use

Tags

YFP

Expression

Yeast

Mutation

D23A mutation in TDP-43 sequence

Promoter

Available since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
pRS423-FjTAL(JBEI-15466)

Plasmid

#87936

Purpose

Expression in S.cerevisiae of FjTAL

Depositor

Dominique Loque

Article

Eudes et al Microb Cell Fact. 2016 Nov 21;15(1):198.

Insert

FjTAL

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCYC1pr-GNSI (LEU)

Plasmid

#111450

Purpose

GNSI is a synthetic, genetically-encoded reporter that allows rapid plate-based assessment of AP-3 functional deficiency, using either colorimetric or growth phenotype readouts.

Depositor

Alexey Merz

Article

Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1

Inserts

CYC1 Promoter (CYC1 Budding Yeast)
Nyv1 Cytosolic Domain (aa 6-230)
Snc1 Transmembrane Domain (TMD)
SUC2 CDS
SUC2 terminator

Use

Tags

mGFP5

Expression

Yeast

Mutation

M109I (Naturally occurring SNP) and The first 21 …

Promoter

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P3nmt1-GFP

Plasmid

#39289

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Expression

Mutation

-1163 to +6 of nmt1 locus

Promoter

Available since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHL

Plasmid

#79614

Purpose

Expresses dCas9-SH3 and PmCDA1-SHL in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Inserts

SpCas9
PmCDA1

Use

Tags

SH3 domain and SHL

Expression

Yeast

Mutation

D10A and H840A for nuclease deficient Cas9

Promoter

pGal1 and pGal10

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pJZC518

Plasmid

#62277

Purpose

Yeast dCas9 expression plasmid

Depositor

Stanley Qi, Wendell Lim

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

dCas9

Use

Tags

Expression

Yeast

Mutation

Has mutations in the RuvC1 and HNH domains to ren…

Promoter

pTdh3

Available since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

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3413 pBF339 Bcl-XL

Plasmid

#8773

Depositor

Stanley Korsmeyer

Article

Gross et al Mol Cell Biol 2000 May;20(9):3125-36.

Insert

Bcl-XL (Bcl2l1 Mouse)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pJZC519

Plasmid

#62278

Purpose

Yeast dCas9-VP64 expression plasmid

Depositor

Stanley Qi, Wendell Lim

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

dCas9-VP64

Use

Tags

Has a VP64 fusion

Expression

Yeast

Mutation

Has mutations in the RuvC1 and HNH domains to ren…

Promoter

pTdh3

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pJZC595

Plasmid

#62322

Purpose

Expresses MCP-VP64 and dCas9 in Yeast cells

Depositor

Stanley Qi, Wendell Lim

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

MCP-VP64
dCas9

Use

Tags

VP64

Expression

Yeast

Mutation

Has nuclease-inactivating mutations in the RuvC1 …

Promoter

Tdh3 and pAdh

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pJT131_GalL_cCDA1-NGBE3

Plasmid

#145095

Purpose

Expressing base editor cCDA1-NGBE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

cCDA1-NGBE3

Use

CRISPR

Tags

Expression

Yeast

Mutation

spCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…

Promoter

GalL

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pRS306Sup35R1

Plasmid

#17715

Depositor

Susan Lindquist

Article

Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

Insert

pRS306Sup35R1 (SUP35 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

An pRS306 based integrating plasmid designed for …

Promoter

Available since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

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pTH397-SUP45-I222S

Plasmid

#29388

Depositor

Tobias von der Haar

Article

Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

Insert

SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

I222S mutant gene (corresponding to the sup45-2 a…

Promoter

Available since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
pNIA-CEN-Venus-FLAG-LINXa4-Bre1

Plasmid

#73618

Purpose

Visualizing the nuclear translocation of LINXa4-Bre1 in yeast.

Depositor

Brian Kuhlman

Article

Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

Insert

Venus

Use

Synthetic Biology

Tags

FLAG

Expression

Yeast

Mutation

Bre1 point mutations K8A, K9A and K11A

Promoter

ADH1

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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