Addgene

We narrowed to 6,564 results for: ski

Showing: 3781 - 3800 of 6564 results

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AAV.CBA.YFP.miR-E_shSrpk3-3

Plasmid

#180402

Purpose

Producing AAV that encodes mouse Srpk3 shRNA-3 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shSrpk3-3 (Srpk3 Mouse)

Use

AAV and RNAi

Tags

Expression

Mutation

Promoter

CBA

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pEY73

Plasmid

#191070

Purpose

cho-1(3.7-1.7k) fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Eviatar Yemini, Oliver Hobert

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

cho-1(3.7-1.7k) (cho-1 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEY72

Plasmid

#191069

Purpose

cho-1(3.7k) fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Eviatar Yemini, Oliver Hobert

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

cho-1(3.7k) (cho-1 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSF11-wmiRFP2

Plasmid

#178969

Purpose

Expresses miRFP2 in worm cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

wmiRFP2

Use

Tags

no

Expression

Worm

Mutation

NO

Promoter

tag-168 promoter

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-DIO-GCaMP6s

Plasmid

#184284

Purpose

Expresses GCaMP6s in genetically defined neurons expressing Cre recombinase

Depositor

William Wisden

Article

Yu et al Nat Neurosci. 2019 Jan;22(1):106-119. do

Insert

CCaMP6s

Use

AAV, Cre/Lox, and Mouse Targeting

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

human synaptophysin

Available since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pFUGW-scrambled

Plasmid

#183455

Purpose

This control vector contains a scrambled version of the targeting sequence used in the pFUGW-shRIIα construct

Depositor

Matthew Gold

Article

Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

Insert

scrambled sgRNA

Use

Lentiviral

Tags

Expression

Mutation

Promoter

shRNA: H1 / gene: ubiquitin

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1-His-TEV-OTC_d1_Ub

Plasmid

#170729

Purpose

Scaﬀold design for studying ubiquitin interacting proteins by cryo-EM.

Depositor

Shang-Te Danny Hsu

Article

Chiu et al Biochemistry. 2021 Apr 13;60(14):1075-10

Insert

E. coli ornithine transcarbamylase (OTC), fused to human ubiquitin

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

The C-terminus of OTC was truncated by removing o…

Promoter

T7

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.TRE.dTomato.miR-ctrl.PGK.sfGFP.P2A.Tet3G

Plasmid

#169316

Purpose

Doxycycline-inducible expression of miR-ctrl with dTomato as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

miR-ctrl

Use

Lentiviral

Tags

dTomato

Expression

Mutation

Promoter

TRE

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.mTagBFP2.miR-ctrl

Plasmid

#169312

Purpose

Constitutive overexpression of miR-ctrl with mTagBFP2 as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

miR-ctrl

Use

Lentiviral

Tags

TagBFP2

Expression

Mutation

Promoter

SFFV

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pIRES-EGFP-TPD53

Plasmid

#172461

Purpose

Expression of untagged Tumor Protein D52 like 1 (TPD53/TPD52L1) and GFP

Depositor

Stephen Royle

Article

Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

TPD53 (TPD52L1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pIRES-EGFP-TPD54

Plasmid

#172459

Purpose

Expression of untagged Tumor Protein D52 like 2 (TPD54/TPD52L2) and GFP

Depositor

Stephen Royle

Article

Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

TPD54 (TPD52L2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pGMC00008

Plasmid

#166724

Purpose

sgRNA cloning backbone

Depositor

Raj Chari

Article

Choi et al Nat Commun. 2020 Jun 1;11(1):2718. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer10-UBC9-sh3

Plasmid

#168987

Purpose

Expresses RFP cDNA and UBC9 shRNA 3 in mammalian cells

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Ubiquitin Conjugating Enzyme 9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ubc promoter

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pQE30-HypocratesCS

Plasmid

#161957

Purpose

Bacterial expression of inactivated control version of genetically encoded biosensor for (pseudo)hypohalous acids and their derivatives

Depositor

Dmitry Bilan

Article

Kostyuk et al Nat Commun. 2022 Jan 10;13(1):171. doi:

Insert

HypocratesCS

Use

Tags

6xHIs tag

Expression

Bacterial

Mutation

changed cysteine 355 to serine

Promoter

T5

Available since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-SNAP-tag_dead_mt

Plasmid

#167270

Purpose

Overexpression of the dead variant (C145A) of SNAP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SNAP-tag

Use

Tags

His x10

Expression

Bacterial

Mutation

C145A

Promoter

T7

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-SNAP-tag_fast_dead_mt

Plasmid

#167272

Purpose

Overexpression of the fast (E30R) dead variant (C145A) of SNAP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SNAPf-tag

Use

Tags

His x10

Expression

Bacterial

Mutation

E30R-C145A

Promoter

T7

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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miniABEMax V82G wt

Plasmid

#135362

Purpose

Expression of nSpyCas9 with N-terminal miniABEMax (V82G)

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

miniABEMax nSpyCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

(V82G)

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCas9FnCpf1GG

Plasmid

#131013

Purpose

Backbone plasmid for generating CRISPR arrays for composite array utilized by SpCas9 and FnCas12a using CRATES. Contains a GFP-dropout cassette and two direct repeats.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Inserts

direct repeat of SpCas9
GFP expression cassette
direct repeat of FnCas12a

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pJET1.2_Nkx3-2_Anti-Sense

Plasmid

#124438

Purpose

Plasmid for anti-sense in situ probe in vitro transcription

Depositor

Yingguang Frank Chan

Article

Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

Insert

NK3 homeobox 2 (Nkx3-2 Mouse)

Use

In situ probe

Tags

Expression

Mutation

Promoter

T7

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pJET1.2_Nkx3-2_Sense

Plasmid

#124437

Purpose

Plasmid for sense in situ probe in vitro transcription

Depositor

Yingguang Frank Chan

Article

Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

Insert

NK3 homeobox 2 (Nkx3-2 Mouse)

Use

In situ probe

Tags

Expression

Mutation

Promoter

T7

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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