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pEQ1100
Plasmid#83937Purposeexpress eGFP with a C-terminal 6X his tag in eukaryotic cellsDepositorInsertEGFP
UseTags6xHIS, V5, and biotinylation signalExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHL
Plasmid#64171Purposeharbours marker genes HIS3 and LEU2DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK_proNR2_LGK.1
Plasmid#81161PurposeExpresses levoglucosan kinase variant LGK.1 with promoter proNR2DepositorInsertLevoglucosan kinase
UseTagsHis6xExpressionBacterialMutationLGK.1 (L140I,S142A,A373C)PromoterproNR2Available sinceSept. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLK
Plasmid#64186Purposeharbours marker genes LEU2 and LYS2DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc4-HPCNDHFR
Plasmid#63998PurposePosition 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsProtein C (HPC); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc4-HPCDHFR
Plasmid#63993PurposePosition 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsProtein C (HPC)ExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc3-DHFRCYD
Plasmid#63988PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsCovalent Yet Dissociable (CYD) [AGTEFCA]ExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc3-HPCDHFR
Plasmid#63975PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsProtein C (HPC)ExpressionBacterialMutationPromoterT7Available sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc2-HPCNDHFR
Plasmid#63962PurposePosition 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsProtein C (HPC); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc3-FLAGDHFR
Plasmid#63974PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsFLAGExpressionBacterialMutationPromoterT7Available sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pST50Trc4-STRDHFR
Plasmid#63991PurposePosition 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsStrep II (STR)ExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc3-HPCNDHFR
Plasmid#63980PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsProtein C (HPC); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pST50Trc2-DHFRCYD
Plasmid#63970PurposePosition 2 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsCovalent Yet Dissociable (CYD) [AGTEFCA]ExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc3-FLAGHISNDHFR
Plasmid#63983PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis & FLAG; TEV cleavableExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc2-HPCDHFR
Plasmid#63957PurposePosition 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsProtein C (HPC)ExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc1-HPCNDHFR
Plasmid#63944PurposePosition 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsProtein C (HPC); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc1-DHFRCYD
Plasmid#63952PurposePosition 1 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsCovalent Yet Dissociable (CYD) [AGTEFCA]ExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only
