Addgene

We narrowed to 8,107 results for: sgRNA

Showing: 361 - 380 of 8107 results

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MYC sgRNA4

Plasmid

#100556

Purpose

Expresses MYC sgRNA4. Target sequence: GTAATTCCAGCGAGAGGCAG

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

MYC sgRNA4

Use

Tags

Expression

Mutation

Promoter

U6

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pEX-A-U6-MiSgRNA_PuroR

Plasmid

#84781

Purpose

Expresses minor satellite-specific sgRNA.

Depositor

Heinrich Leonhardt

Article

Schmidtmann et al Nucleus. 2016 Sep 27:0.

Insert

minor satellite-specific sgRNA

Use

CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

U6

Available since

Nov. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-trmI

Plasmid

#89955

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting trmI.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

trmI gRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-pfkB

Plasmid

#102284

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting pfkB.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

pfkB gRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-xylA

Plasmid

#89964

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting xylA.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

xylA gRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-lpoB

Plasmid

#89959

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting lpoB.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

lpoB gRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-sgRNA_Dgcr8_3

Plasmid

#73527

Purpose

Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

DGCR8

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
pX330-sgRNA_Dgcr8_4

Plasmid

#73528

Purpose

Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

DGCR8

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
pX330-sgRNA_Dgcr8_2

Plasmid

#73526

Purpose

Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

DGCR8

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only
sgRNA1_Cas9-hGem_Smc4

Plasmid

#217667

Purpose

expresses Cas9-hGem and guideRNA for tagging of SMC4

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

sgRNA1 targeting SMC4 C-terminal (SMC4 Human)

Use

CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

U6

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2 NT sgRNA3

Plasmid

#164929

Purpose

Expresses Non-targeting sgRNA3 control in mammalian cells

Depositor

Ji Luo

Article

Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

Insert

Non-targeting sgRNA3 (verified for knockout)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EFS promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2 NT sgRNA5

Plasmid

#164930

Purpose

Expresses Non-targeting sgRNA5 control in mammalian cells

Depositor

Ji Luo

Article

Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

Insert

Non-targeting sgRNA5 (verified for knockout)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EFS promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD3 KO sgRNA #1

Plasmid

#207105

Purpose

pX330 based plasmid for expression of Cas9 and the CGCTATCAAGATTTATACCT sgRNA to target the SHLD3 locus..

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

CGCTATCAAGATTTATACCT

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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MDC1 KO sgRNA

Plasmid

#207103

Purpose

pX330 based plasmid for expression of Cas9 and the GGTGTAACGTGGAGCCAGTA sgRNA to target the MDC1 coding sequence.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GGTGTAACGTGGAGCCAGTA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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U6-sgRNAs-Crym

Plasmid

#200067

Purpose

For the knock down of Crym with an astrocyte specific mCherry expression

Depositor

Baljit Khakh

Article

Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

Insert

U6-3xsgRNA-Crym

Use

AAV, CRISPR, and Mouse Targeting

Tags

GfaABC1D-mCherry

Expression

Mutation

Promoter

U6, GfaABC1D

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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U6-sgRNA-GFP

Plasmid

#200068

Purpose

For the knock down of GFP with an astrocyte specific mCherry expression

Depositor

Baljit Khakh

Article

Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

Insert

U6-sgRNA-GFP

Use

AAV, CRISPR, and Mouse Targeting

Tags

GfaABC1D-mCherry

Expression

Mutation

Promoter

U6, GfaABC1D

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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HaloTag KO sgRNA

Plasmid

#207102

Purpose

pX330 based plasmid for expression of Cas9 and the GTCGATGTTGGTCCGCGCGA sgRNA to target the HaloTag coding sequence.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GTCGATGTTGGTCCGCGCGA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD3 KO sgRNA #2

Plasmid

#207106

Purpose

pX330 based plasmid for expression of Cas9 and the CTGAAGGAACAGACTAATTC sgRNA to target the SHLD3 locus..

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

CTGAAGGAACAGACTAATTC

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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53BP1 KO sgRNA

Plasmid

#207104

Purpose

pX330 based plasmid for expression of Cas9 and the AGATTCTCAGCCTGAAAGCC sgRNA to target the 53BP1 coding sequence.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

AGATTCTCAGCCTGAAAGCC

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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U6-sgRNA-hcr3

Plasmid

#193660

Purpose

Expression of tandem pre-sgRNA array hcr3 for LbCas12a

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

U6-CFTR-sgRNA-KLF4-sgRNA-TET1-sgRNA-tRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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