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Plasmid#191242PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
UseTagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-CP8B1
Plasmid#191240PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
UseTagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-TYK2VF/PA-VSV
Plasmid#139351PurposeExpression of the double mutant of TYK2-V678F-P1104ADepositorInsertTYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
UseTagsVSVExpressionMammalianMutationV362F-V678F-P1104APromoterCMVAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2-zfTpo
Plasmid#168198PurposeExpress zebrafish thrombopoietin (Tpo), mRNA synthesisDepositorInsertThrombopoietin (thpo Zebrafish)
UseVertebrate expression (zebrafish, chicken, xenopu…TagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-JAK1K908E-VSV
Plasmid#139357PurposeExpression of kinase-dead JAK1-K908E proteinDepositorInsertJAK1 cDNA with 300nt of 5' UTR (JAK1 Human)
UseTagsVSVExpressionMammalianMutationK908EPromoterCMVAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-JAK1P1084A-VSV
Plasmid#139358PurposeExpression of the catalytically impaired JAK1-P1084A mutant (corresponding to TYK2-P1104A)DepositorInsertJAK1 cDNA with 300nt of 5' UTR (JAK1 Human)
UseTagsVSVExpressionMammalianMutationP1084APromoterCMVAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
6-His-MBP-TEV-FnCpf1
Plasmid#90094PurposeBacterial expression plasmid for protein purificationDepositorInsertFnCpf1 (humanized)
UseTags3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
HL 716
Bacterial Strain#60368PurposeLim Lab StrainDepositorBacterial ResistanceNoneAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
HL 770
Bacterial Strain#60369PurposeLim Lab StrainDepositorBacterial ResistanceNoneAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
HL 862
Bacterial Strain#60370PurposeLim Lab StrainDepositorBacterial ResistanceNoneAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
HL 865
Bacterial Strain#60371PurposeLim Lab StrainDepositorBacterial ResistanceNoneAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
HL 1128
Bacterial Strain#60372PurposeLim Lab StrainDepositorBacterial ResistanceNoneAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
HL 1188
Bacterial Strain#60373PurposeLim Lab StrainDepositorBacterial ResistanceNoneAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
HL 2752
Bacterial Strain#60374PurposeLim Lab StrainDepositorBacterial ResistanceNoneAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
HL 3338
Bacterial Strain#60376PurposeLim Lab StrainDepositorBacterial ResistanceNoneAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAc-His-zfTpo
Plasmid#64309PurposeExpress soluble His-tagged zebrafish thrombopoietin in insect cellsDepositorInsertThrombopoietin (thpo Zebrafish)
UseTags(His)6ExpressionInsectMutationThrombopoietin starts at AA37 (AA1-36 encode the …PromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
HL 2729 (=HL 716 + rhyB knockout_KanR)
Bacterial Strain#30030DepositorBacterial ResistanceKanamycinSpeciesEscherichia coliAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
HL 849 (=HL 716 + micC knockout_KanR)
Bacterial Strain#30009DepositorBacterial ResistanceKanamycinSpeciesEscherichia coliAvailable sinceJuly 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
HL 713
Bacterial Strain#52931PurposeMG 1655 + lacIq_KanR inserted into the chromosome at the intS siteDepositorBacterial ResistanceKanamycinSpeciesEscherichia coliAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
HL 751
Bacterial Strain#52932PurposeMG 1655 + lacIq inserted into the chromosome at the intS site + Δhfq_KanRDepositorBacterial ResistanceKanamycinSpeciesEscherichia coliAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only