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Plasmid#112895PurposeNon-integrating lentiviral co-packaging plasmid designed to reduce multiple integrations and recombination during transduction of nucleic acid librariesDepositorArticleTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoternoneAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only
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CROPseq-multi-EBFP2-NLS (pRW1447)
Plasmid#216219PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi construct with nuclear-localized EBFP2 expressionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-3Myc
Plasmid#221046PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsert3xMyc epitopes
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-3FLAG
Plasmid#221045PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsert3xFLAG epitopes
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX311_CdTag-HA_EmptyVector
Plasmid#221468PurposeGateway backbone for C-terminal dTag cloningDepositorTypeEmpty backboneUseLentiviralTagsFKBP F36VExpressionMammalianMutationPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX307_CdTag-HA_EmptyVector
Plasmid#221467PurposeGateway backbone for C-terminal dTag cloningDepositorTypeEmpty backboneUseLentiviralTagsFKBP F36VExpressionMammalianMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX302_CdTag-HA_EmptyVector
Plasmid#221465PurposeGateway backbone for C-terminal dTag cloningDepositorTypeEmpty backboneUseLentiviralTagsFKBP F36VExpressionMammalianMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX304_CdTag-HA_EmptyVector
Plasmid#221466PurposeGateway backbone for C-terminal dTag cloningDepositorTypeEmpty backboneUseLentiviralTagsFKBP F36VExpressionMammalianMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUBC-SV40NLS-PfV-Sapphire-IRES-H2B-mCherry
Plasmid#203652PurposeMammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a secondary translation initiation site for H2B-mCherry expression.DepositorArticleInsertPfV
UseLentiviralTagsSapphire-IRES-H2B-mCherryExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBTK1056
Plasmid#209546PurposeBTK Type 4 antibiotic resistance cassette for Golden Gate assemblyDepositorInsertType 4 Kanamycin resistance cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 12, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC-HIS6-GST-TEV-GG
Plasmid#215801PurposeCloning of target gene with N terminal His6 GST tag and TEV cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
CD Ugi1
Plasmid#177194PurposeGreengate entry vector pGGC000 modified to contain bladderwort insulator 1DepositorInsertUtricularia gibba insulator 1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHR hEF1A-BFP-P2A-rtTA-WPRE
Plasmid#223288PurposeA lentiviral vector expressing BFP and the TetON transcription factor rtTA.DepositorInsertBFP-P2A-rtTA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAP2
Plasmid#171208Purposebacterial expression for purification of PGL-3, allowing cleavage for MBP tag using TEV proteaseDepositorInsertpgl-3 (pgl-3 Nematode)
UseTagsMBP::HIS::TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
nanobody(AntiGFP)-HaloTag-iLID
Plasmid#191453PurposeLocalizing iLID with GFP-tagged proteinsDepositorInsertnanobody(AntiGFP)-HaloTag-iLID
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNIC-HIS6-MBP-TEV-GG
Plasmid#215800PurposeCloning of target gene with N terminal His6 MBP tag and TEV CleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPOTv7-blast-10FLAG
Plasmid#181940PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsert10xFLAG
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pTU2-AmyE-Pveg-eGFP
Plasmid#112792PurposeExpresses GFP under the control of Pveg promoter and it is integrated into AmyE locus. Used to test correct transformationDepositorArticleInsertPveg-eGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-10HA
Plasmid#181941PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsert10xHA
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only