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Dox nontargeting shRNA mKate
Plasmid#155292PurposeLentivirus for doxycycline inducible expression of nontargeting shRNA, mKate marker, based on Addgene 11652DepositorInsertNontargeting shRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
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Lentiviral nontargeting shRNA RFP i670
Plasmid#155287PurposeLentiviral expression of nontargeting shRNA, RFP i670 expression, based on Addgene 12247DepositorInsertNontargeting shRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
Dox nontargeting shRNA GFP
Plasmid#155290PurposeLentivirus for doxycycline inducible expression of nontargeting shRNA, GFP marker, based on Addgene 11652DepositorInsertNontargeting shRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
dSaCas9-KRAB
Plasmid#205250PurposeIn vitro transcription plasmid for production of dSaCas9-KOX1 KRAB mRNA for transcriptional activation (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #106219.DepositorInsertdSaCas9-KRAB
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-PB-R4R3
Plasmid#139518PurposeProvides two PiggyBac transposon sequences between gene cassettes in the destination vectorDepositorTypeEmpty backboneUsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
RedTrackCMV
Plasmid#50957PurposeA backbone for adenovirus creation by the AdEasy method, similar to AdTrackCMV (Addgene Plasmid # 16405), except it co-expressed mRFP instead of eGFPDepositorTypeEmpty backboneUseAdenoviralTagsExpressionMutationPromoterCMVAvailable sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-Bsa4-TF
Plasmid#61689PurposeE. coli expression vector based on pNIC28-Bsa4 (addgene #26103) with the TEE and trigger factor sequences insertedDepositorTypeEmpty backboneUseTagsHIS and Trigger FactorExpressionBacterialMutationPromoterT7Available sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Bla/GFP
Plasmid#198221PurposeTransposon vector (Addgene #60510) for doxycycline-inducible expression of EGFPDepositorInsertEGFP
UseTransposonTagsExpressionMammalianMutationNonePromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
mito-meGFP
Plasmid#172481PurposemeGFP targeted to the mitochondrial matrix with a human COX8 presequenceDepositorInsertMito
UseTagsmeGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-mCherry-WPREpA
Plasmid#177328PurposeControl vector for Flp-dependent DREADD constructsDepositorInsertmCherry
UseAAV; Flp-frtTagsExpressionMammalianMutationPromoterHuman Synapsin-1Available sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
p221-RV-dlx-dio-Tdtomato
Plasmid#185697PurposeRetroviral vector expression cre-dependent membrane tagged Tdtomato driven by the mDlx promoterDepositorInsertmembrane Tdtomato
UseCre/Lox and RetroviralTagsGap43 palmitoylationExpressionMutationPromotermDlxAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-TET-PH-lox66FRT
Plasmid#139561PurposepiggyBac expression vector with TET-ON system and puromycin selectionDepositorInsertPGK-puroR-pA cassette, inserted in PB-TET vector
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-TET-ZH-lox66FRT
Plasmid#139563PurposepiggyBac expression vector with TET-ON system and Zeocin selectionDepositorInsertPGK-ZeoR-pA cassette, inserted in PB-TET vector
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-TET-BH-lox66FRT
Plasmid#139562PurposepiggyBac expression vector with TET-ON system and Blasticidin selectionDepositorInsertPGK-BSD-pA cassette, inserted in PB-TET vector
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL21-decoder
Plasmid#159448PurposeInsert: Pact1-lexA-ER-B112-phosphodegron-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is degraded upon addition of alpha factor. Backbone: pRG201 (addGene). Marker: Met15.DepositorInsertPact1-lexA-ER-B112-phosphodegron-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoteract1Available sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL38-decoder
Plasmid#159452PurposeInsert: Pfus1tet-lexA-ER-B112-tCyc1.The transcription factor LexA-ER-B112 (see addgene FRP880) is inducible by alpha factor, and repressible by TetR. Backbone: pRG235 (addgene). Marker: Leu2DepositorInsertPfus1tet-LexA-ER-B112-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPfus1tetAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur/GDI1-splitTurboID
Plasmid#198219PurposeTransposon vector (Addgene #60523) for expression of part of TurboID (up to E256) and GID1, for gibberellic acid-inducible biotin labelingDepositorInsertGID1
UseTransposonTags5’ part of TurboID, up to E256ExpressionMammalianMutationPromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUltra-Plus-H2B-EGFP
Plasmid#161776Purpose3rd generation lentiviral bicistronic vector for mammalian expression of H2B-EGFP and one gene of interestDepositorInsertH2B-EGFP from Addgene Plasmid #26790
UseLentiviral; BicistronicTagsEGFPExpressionMammalianMutationPromoterUbC and UbC promoterAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only
