Addgene

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pWZ854

Plasmid

#208116

Purpose

mSwAP-In marker cassette 2 with 5’PB-mNeongreen-HPRT1-Neo

Depositor

Jef Boeke

Article

Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

Insert

mNeonGreen-HPRT1-Neo

Use

Tags

T2A

Expression

Bacterial and Mammalian

Mutation

Promoter

EF1alpha

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pWZ852

Plasmid

#208114

Purpose

mSwAP-In marker cassette 1 with mScarlet-HygTK

Depositor

Jef Boeke

Article

Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

Insert

mScarlet-HygTK

Use

Tags

T2A

Expression

Bacterial and Mammalian

Mutation

Promoter

EF1alpha

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pWZ699

Plasmid

#208117

Purpose

Payload yeast assembly vector with TK-mActb on backbone

Depositor

Jef Boeke

Article

Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

Type

Empty backbone

Use

Synthetic Biology; Copy number inducible

Tags

Expression

Bacterial, Mammalian, and Y…

Mutation

Promoter

Human PGK1 for thymidine kinase

Available since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pF CAG luc puro

Plasmid

#67501

Purpose

Constitutive expression of firefly luciferase in mammalian cells

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

Firefly luciferase

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FDIO-ArchT-GFP

Plasmid

#124640

Purpose

AAV-mediated FLPo-dependent expression of ArchT-GFP under EF1a promoter

Depositor

David Dupret

Article

Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

Insert

ArchT

Use

AAV; Flp/frt

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CNTF-HA

Plasmid

#195517

Purpose

Expresses HA-tagged CNTF in mammalian cells

Depositor

Kevin Park

Article

Ribeiro et al Exp Neurol. 2022 Sep;355:114147. doi: 10

Insert

Ciliary neurotrophic factor (CNTF Human)

Use

AAV

Tags

HA-tag and NGF signal peptide

Expression

Mammalian

Mutation

Promoter

UbC promoter with beta-globin intron

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLP-PIGA3 (pLP305)

Plasmid

#196992

Purpose

Big-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites, sleeping beauty ITRs and BsmBI homology arms cloning sites.

Depositor

Matthew Maurano

Article

Brosh et al Mol Cell. 2023 Apr 6;83(7):1140-1152.e7.

Type

Empty backbone

Use

Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Nkx2.5

Plasmid

#72689

Purpose

Doxycyline regulated expression of Nkx2.5 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Nkx 2.5 (Nkx2-5 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV-Doxycycline

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Baf60c

Plasmid

#72691

Purpose

Doxycyline regulated expression of Baf60c in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Brg 1 (SMARCD3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV-Doxycycline

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pUT1163-HQ858707

Plasmid

#27753

Depositor

Erich Grotewold, John Gray

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

bZIP transcription factor from DV507461

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Tbx5

Plasmid

#72688

Purpose

Doxycyline regulated expression of Tbx5 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Tbx 5 (Tbx5 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV-Doxycycline

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pUT1109-HQ858666

Plasmid

#27719

Depositor

Erich Grotewold, John Gray

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

C2C2-CO-like transcription factor from DT643534

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pUT1141-HQ858685

Plasmid

#27731

Depositor

Erich Grotewold, John Gray

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

bZIP transcription factor from EE179215

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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pUT1439-HQ858741

Plasmid

#27823

Depositor

Erich Grotewold, John Gray

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

NAC transcription factor from DT947867

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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pUT1769-HQ858783

Plasmid

#27865

Depositor

Erich Grotewold, John Gray

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

DBP transcription factor from EB701836

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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P789_pY026_EnAsCpf1_CLYBL_T1_catRNA

Plasmid

#202760

Purpose

Encodes for CRISPR-Cas12a (EnAsCpf1) with gRNA for targeting the CLYBL safe harbor site

Depositor

Karl Wahlin

Article

Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

Insert

huAsCpf1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pGG442

Plasmid

#165616

Purpose

Vector for expression of SpCas9-Zif268 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t (KanR cassette in place of the PID)

Depositor

Marcus Noyes

Article

Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

Insert

TEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t

Use

CRISPR

Tags

3x HA, SV40 NLS, Zif268, and c-Myc-like NLS

Expression

Yeast

Mutation

Corrected homology relative to wild type SpCas9 (…

Promoter

TEF1

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pUT1105-HQ858668

Plasmid

#27715

Depositor

Erich Grotewold, John Gray

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

PHD transcription factor from DV543172

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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pUT1106-HQ858670

Plasmid

#27716

Depositor

Erich Grotewold, John Gray

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

Homeobox HB transcription factor CDS from DR804210

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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pUT1107-HQ858671

Plasmid

#27717

Depositor

Erich Grotewold, John Gray

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

CPP transcription factor from EB166014

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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