-
Plasmid#166084PurposePlasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of TDH3 for double stranded break formation in yeast.DepositorInsertPromoter of TDH3 (TDH3 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMGAHisTev5B
Plasmid#37221DepositorInserteIF5B
UseTagsHisExpressionMutationN-term truncation (contains aa 396-1002)PromoterAvailable sinceAug. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTYB2-eIF1
Plasmid#37217DepositorInserteIF1
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTYB2-eIF5
Plasmid#37219DepositorInserteIF5
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
-
-
pMN252
Plasmid#32177DepositorInsertrpsL, FRT-hyg-FRT
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
p404MET25
Plasmid#17420DepositorInsertMET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationMET25 promoter is between SacI and XbaI restricti…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTYB2-eIF1A
Plasmid#37218DepositorInserteIF1A
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
p406MET25
Plasmid#17421DepositorInsertMET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationMET25 promoter is between SacI and XbaI restricti…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
peAA.11
Plasmid#112149Purpose"Vector AA"DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUK21-deltaBB
Plasmid#51658PurposeBacterial cloning vector based on pUK21 (Kan)DepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_RPL39
Plasmid#166078PurposePlasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RPL39 for double stranded break formation in yeast.DepositorInsertPromoter of RPL39 (Overlaps with 5'UTR and first base of gene) (RPL39 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_YDR344C
Plasmid#166072PurposePlasmid for constituive spCas9 expression and tet-inducible expression of sgRNA targeting intergenic site near HXT6 (within YDR344C) for double stranded break formation in yeast.DepositorInsertIntergenic site near HXT6 (in YDR344C, possible dubious ORF)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
peDK.11
Plasmid#112150Purpose"Vector DK"DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBSIIKS_HIS3_I
Plasmid#109062Purposeyeast shuttle vector with deletable CEN6-ARSH4 cassette in pBIIKS backbone. LoxP site upstream of HIS3. For teaching purposes.DepositorInsertHIS3 (HIS3 Zygosaccharomyces rouxii, Budding Yeast)
UseCre/LoxTagsExpressionBacterial and YeastMutationPromoterHIS3Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC21-deltaBB
Plasmid#51657PurposeBacterial cloning vector based on pUC21 (Amp)DepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT040
Plasmid#67640PurposeURA3 vector for cloning guide sequences using BclI-SwaI sites into guide RNA expression cassetteDepositorInsertsingle guide RNA expression cassette
UseCRISPRTagsExpressionYeastMutationPromoterpSNR52Available sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cas9-NAT
Plasmid#64329PurposeCas9 expression cassette carried by a yeast single-copy episome plasmidDepositorInsertHuman-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLM494
Plasmid#100539PurposeCEN/ARS plasmid harboring expression cassettes for crtI, crtYB, crtE and tHMG1 of the β-carotene pathway, each flanked by loxPsym sites.DepositorInsertsGGPP synthase
phytoene desaturase
phytoene-beta carotene synthase
HMG CoA reductase
UseCre/Lox and Synthetic BiologyTagsExpressionYeastMutationPromoterPGK1, TDH3, TIP1, and ZEO1Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only