-
Plasmid#155338PurposeBinary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling GUS. It constitutively expresses dsRed and a plant selection cassette.DepositorInsertRB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-dsRed-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-GUS-POpto_LB
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.0-TERT G228A
Plasmid#84926Purposeluciferase reporter for mutant TERT promoterDepositorInsertTERT promoter (TERT Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationG228APromoterTERTAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX317-VRK1-K179E
Plasmid#199644PurposeExpresses kinase-inactive VRK1 (K179E)DepositorInsertVRK1 (VRK1 Human)
UseLentiviralTagsExpressionMutationK179EPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-7:SEC61B-mEGFP
Plasmid#87426PurposeHomology arms and linker-mEGFP sequence for N-terminus tagging of human SEC61BDepositorInsertSEC61B Homology Arms with mEGFP-linker (SEC61B Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceMarch 15, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
DYR1A_HUMAN_D0
Plasmid#79690PurposeThis plasmid encodes the kinase domain of DYR1A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertDYRK1A (DYRK1A Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
ABL1_HUMAN_D0
Plasmid#79727PurposeThis plasmid encodes the kinase domain of ABL1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertABL1 (ABL1 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-SwitchON-sgVRK1
Plasmid#199638PurposeTamoxifen-inducible expression of sgRNA targeting VRK1DepositorInsertN/A (VRK1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiRCas9-CUG
Plasmid#104183PurposeLentiviral transfer vector that carries U6-driven sgRNA targeting CUG repeats using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)DepositorInsertU6-CUGsgRNA, EFS-PIN-dCas9
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-mKOR
Plasmid#179285PurposeExpress mouse kappa opioid receptor (GFP tagged) in mammalian cellsDepositorInsertkappa opioid receptor (Oprk1 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHis-LMW_FGF2
Plasmid#157657PurposeExpresses FGF2 in E.coli cellDepositorInsertFibroblast growth factor 2 (FGF2 Human)
UseTagsHis tagExpressionBacterialMutationchanged Cystein 211,229 to serine, deleted amino …PromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR-53BP1 1-1972 28A
Plasmid#53447PurposeRecombinational donor/master vectorDepositorInsert53BP1 (TP53BP1 Human)
UseGatewayTagsExpressionMutationI914T mutation; All 28 SQ/TQ motifs in N-term of …PromoterAvailable sinceDec. 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
Chr1q_Cassette-Integration_gRNA
Plasmid#195126PurposegRNA in a third generation Cas9 vector with GFP, targeting location downstream of Chr1q_centromere-targeting_gRNA for positive-negative selection cassette integrationDepositorInsertChr1q gRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO-sh-Nix
Plasmid#100770PurposeLentiviral expression of shRNA targeting NixDepositorInsertLenti-sh-Nix
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
Dyn1(K44A)-mRFP
Plasmid#55795PurposeDominant negative Dynamin 1 mutant tagged with mRFPDepositorInsertDynamin-1(K44A)-mRFP (Dnm1 Discosoma sp., Rat)
UseTagsmRFPExpressionMammalianMutationDynamin-1(K44A)-mRFP was produced from Dynamin-1(…PromoterCMVAvailable sinceSept. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
p23-NES-LwaCas13a-msfGFP-NES-Flag
Plasmid#165070Purposeoverexpression of LwaCas13a in human cellsDepositorInsertLwaCas13a
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL4.0-TERT G250A
Plasmid#84925Purposeluciferase reporter for mutant TERT promoterDepositorInsertTERT promoter (TERT Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationG250APromoterTERTAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hWWTR1-W1K)-PGKpuro2AmCherry-W
Plasmid#163176PurposeLentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of mCherry tagDepositorInsertWWTR1 (WWTR1 Human)
UseCRISPR and LentiviralTagsmCherryExpressionMammalianMutationPromoterU6Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBullet-tgn-c
Plasmid#53072Purposedestination vector with ECFP-trans golgi network marker (VTI12) for tagged fluorescent protein (C-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETM6-G6-vioABE-4A6-vioC
Plasmid#73439PurposeDeoxyviolacein pathway (vioABEC) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE) and 4A6 (vioC) for orthogonal flux redirection.DepositorInsertPG6-vioABE-P4A6-vioC
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterPG6 and P4A6 (orthogonal T7-lac variants)Available sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 WT 2014 EBOV Delta-Mucin-Like-Domain
Plasmid#86021PurposeEbola Virus (Makona) Glycoprotein in CMV driven mammalian expression vectorDepositorInsertMakona Ebola Virus Glycoprotein
UseTagsExpressionMammalianMutationLacks mucin-like domainPromoterCMV IEAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only