Addgene

We narrowed to 4,926 results for: pcDNA3.1+

Showing: 3761 - 3780 of 4926 results

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pcDNA3-ING4

Plasmid

#13289

Depositor

Curtis Harris

Article

Shiseki et al Cancer Res. 2003 May 15. 63(10):2373-8.

Insert

ING4 (ING4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
Vh-HA-FKBP

Plasmid

#20220

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

FKBP (FKBP1A Human)

Use

Tags

HA and Vh

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
SpyCas9-AIDx

Plasmid

#135343

Purpose

Expression of wildtype nSpyCas9 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSpyCas9-hAIDx

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA Flag PGC1 T177A S538A

Plasmid

#18093

Depositor

Bruce Spiegelman

Article

Jager et al Proc Natl Acad Sci U S A. 2007 Jul 17. 1

Insert

PGC-1a (Ppargc1a Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

T177A S538A

Promoter

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
REEP5-2Strep

Plasmid

#210350

Purpose

Expresses REEP5 fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

REEP5 (REEP5 Human)

Use

Tags

2Strep

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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F-CDK8L

Plasmid

#24762

Depositor

Ronald Conaway, Joan Conaway

Article

Conaway et al FEBS Lett. 2005 Feb 7. 579(4):904-8.

Insert

F-CDK8L (CDK19 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2010

Availability

Academic Institutions and Nonprofits only
pspCas9-ABE-3'UTR-sgRNA-ST2-com vector

Plasmid

#136270

Purpose

Expresses ABE and ST2-com modified sgRNA scaffold

Depositor

Baisong Lu

Article

Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

Insert

ABE and sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pTracer_Cr-TRP1

Plasmid

#64881

Purpose

Expresses CrTRP1 in mammalian cells

Depositor

Sebastián Brauchi

Article

Arias-Darraz et al Plant Cell. 2015 Jan;27(1):177-88. doi:

Insert

Transient receptor potential 1

Use

Tags

eGFP

Expression

Mutation

Promoter

CMV

Available since

June 20, 2016

Availability

Academic Institutions and Nonprofits only
ABEMax nSaCas9

Plasmid

#135364

Purpose

Expresion of SaCas9 nickase (D10A) with ABEMax at the N terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

ABEMax nSaCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

(V82G)

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAL113

Plasmid

#22471

Purpose

Expresses N-terminal domains of PhyB (with Y276H mutation), fused to mCFP and HRas C terminal CAAX palmitoylation sequence. For red-light-induced binding to PIF6.

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

PhyB NT (PHYB Mustard Weed, Mouse)

Use

Synthetic Biology

Tags

10aaLinker-mCFP-HrasCT

Expression

Mammalian

Mutation

Y276H

Promoter

CMV

Available since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-BaLgp140-8his

Plasmid

#100924

Purpose

Mammalian expression plasmid for soluble gp140 from the BaL HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Use

Tags

CD5 leader sequence and Purification tag after D6…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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PCDNA3-N3FLAG-MYB-FL

Plasmid

#105609

Purpose

transient express full length MYB with 3*FLAG tag at N terminal in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

Full length MYB (mouse) (Myb Mouse)

Use

Tags

3*FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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ALIX(1-702)-mNeonGreen

Plasmid

#191193

Purpose

Truncated form of ALIX that lacks C-terminal proline-rich domain; C-terminally tagged with mNeonGreen.

Depositor

Jin Zhang

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

ALIX (1-702) (PDCD6IP Human, Synthetic)

Use

Tags

6xHIS and mNeonGreen

Expression

Mammalian

Mutation

Deletion of ALIX C-terminal PRD.

Promoter

CMV

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA SF-HERC2 F3

Plasmid

#55786

Purpose

Expresses N-terminal 3xFLAG/STREP tagged HERC2 (1700-2700aa)

Depositor

David Chan

Article

Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

Insert

3xFLAG tagged HERC2 (1700aa-2700aa) (HERC2 Human)

Use

Tags

3xFLAG and STREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-ING2

Plasmid

#13294

Depositor

Curtis Harris

Article

Pedeux et al Mol Cell Biol. 2005 Aug . 25(15):6639-48

Insert

ING2 (INGX Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pAL130

Plasmid

#22475

Purpose

Expresses N terminal domain of PIF3 fused to mYFP, for red-light-induced binding to Phy

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

PIF3NT (PIF3 Mustard Weed, Mouse)

Use

Synthetic Biology

Tags

mYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA_RGG-GFP-RGG_withTEVsite

Plasmid

#124939

Purpose

Expresion of RGG_GFP-RGG

Depositor

Matthew Good, Benjamin Schuster, Daniel Hammer

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Use

Tags

GFP

Expression

Mammalian

Mutation

TEV site between RGG and EGFP

Promoter

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA SF-HERC2 F6

Plasmid

#55789

Purpose

Expresses N-terminal 3xFLAG/STREP tagged HERC2 (4421-4834aa)

Depositor

David Chan

Article

Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

Insert

3xFLAG tagged HERC2 (4421-4834aa) (HERC2 Human)

Use

Tags

3xFLAG and STREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2014

Availability

Academic Institutions and Nonprofits only
FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA

Plasmid

#137827

Purpose

MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal WT TEV protease, M cleavage sequence, and tTA transcription factor

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 hML23Cm6

Plasmid

#109368

Purpose

Human melanopsin chimera with intracellular loops 2-3 and C-terminus from mGluR6

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

human Melanopsin-mGluR6 chimera (OPN4, GRM6 Human)

Use

Tags

1D4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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