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Showing: 3741 - 3760 of 13589 results

  1. pET28-RPL3-299~398-mEGFP(A206K)-TwStrep

    Plasmid
    #208627
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa299~398, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 299~398 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 299~398, other residues not included
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208627-plasmid-map-sequence-id-415915

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  2. pET28-RPL3-212~285-mEGFP(A206K)-TwStrep

    Plasmid
    #208629
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa212~285, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 212~285 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 212~285, other residues not included
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208629-plasmid-map-sequence-id-415924

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  6. pET-15b(+) Mortierella verticillate (delta prenylation motif)

    Plasmid
    #170788
    Purpose
    Expresses pET-15b(+) Mortierella verticillate (delta prenylation motif)
    Depositor
    Kalle Gehring
    Insert
    Mortierella verticillate PRL
    Use
    Tags
    N-terminal His-tag
    Expression
    Bacterial
    Mutation
    Deleted nucleotides coding for 4 C-terminal amino…
    Promoter
    AmpR, lacI
    Available since
    Sept. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    170788-plasmid-map-sequence-id-337769

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  7. pET-15b(+) Toxoplasma gondii (delta prenylation motif)

    Plasmid
    #170789
    Purpose
    Expresses pET-15b(+) Toxoplasma gondii (delta prenylation motif)
    Depositor
    Kalle Gehring
    Insert
    Toxoplasma gondii PRL
    Use
    Tags
    N-terminal His-tag
    Expression
    Bacterial
    Mutation
    Deleted nucleotides coding for 4 C-terminal amino…
    Promoter
    AmpR, lacI
    Available since
    Sept. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    170789-plasmid-map-sequence-id-337771

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  8. pET-15b(+) Plasmodium falciparum PRL (Δprenylation motif)

    Plasmid
    #166422
    Purpose
    Express pET-15b(+) Plasmodium falciparum PRL (Δprenylation motif)
    Depositor
    Kalle Gehring
    Insert
    Plasmodium falciparum PRL
    Use
    Tags
    N-terminal His-tag
    Expression
    Bacterial
    Mutation
    Deleted nucleotides coding for 4 C-terminal amino…
    Promoter
    AmpR, lac1
    Available since
    Sept. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    166422-plasmid-map-sequence-id-329461

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  12. pET-15b(+) Acytostelium subglobosum PRL (Δprenylation motif)

    Plasmid
    #166415
    Purpose
    Express pET-15b(+) Acytostelium subglobosum PRL (Δprenylation motif)
    Depositor
    Kalle Gehring
    Article
    Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi
    Insert
    Acytostelium subglobosum PRL
    Use
    Tags
    N-terminal His-tag
    Expression
    Bacterial
    Mutation
    Deleted nucleotides coding for 4 C-terminal amino…
    Promoter
    AmpR, lac1
    Available since
    Sept. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    166415-plasmid-map-sequence-id-329462

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  15. pET-15b(+) Drosophila melanogaster PRL (Δprenylation motif)

    Plasmid
    #166418
    Purpose
    Express pET-15b(+) Drosophila melanogaster PRL (Δprenylation motif)
    Depositor
    Kalle Gehring
    Article
    Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi
    Insert
    Drosophila melanogaster PRL (Prl Fly)
    Use
    Tags
    N-terminal His-tag
    Expression
    Bacterial
    Mutation
    Deleted nucleotides coding for 4 C-terminal amino…
    Promoter
    AmpR, lac1
    Available since
    Sept. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    166418-plasmid-map-sequence-id-329456

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  19. pET29b-AviTag-mEGFP-dspB(E184Q W330Y)-6xHis

    Plasmid
    #176575
    Purpose
    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.
    Depositor
    Mark Nitz
    Article
    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:
    Insert
    Dispersin B
    Use
    Tags
    Avitag, Hexahistidine tag, and mEGFP
    Expression
    Bacterial
    Mutation
    E184Q W330Y
    Promoter
    T7
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    176575-plasmid-map-sequence-id-348273

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  20. pET29b-AviTag(K10A)-eGFP-dspB(E184Q)-6xHis

    Plasmid
    #175788
    Purpose
    Plasmid for overexpression of recombinant AviTagged(nonfunctional), His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.
    Depositor
    Mark Nitz
    Article
    Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12
    Insert
    Dispersin B
    Use
    Tags
    AviTag, Hexahistidine tag, and eGFP
    Expression
    Bacterial
    Mutation
    E184Q
    Promoter
    T7
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    175788-plasmid-map-sequence-id-345285

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Showing: 3741 - 3760 of 13589 results