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Plasmid#188588PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler C-terminal domain, catalytic mutant
UseTagsFLAG-MycExpressionInsectMutationD435A [GAT=>GCC]; E435A [GAA=>GCC]PromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[C399-625]-Gwpep(Mm)
Plasmid#188589PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertGW-peptide (mouse) C-terminally tagged C-terminal domain of Nibbler
UseTagsFLAG-Myc and GWpep mmExpressionInsectMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[C399-625]-Gwpep(Dm)
Plasmid#188590PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertGW-peptide (drosophila) C-terminally tagged C-terminal domain of Nibbler
UseTagsFLAG-Myc and Gwpep dmExpressionInsectMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Gwpep(Dm)-Nbr[C399-625]
Plasmid#188592PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertGW-peptide (drosophila) N-terminally tagged C-terminal domain of Nibbler
UseTagsFLAG-Myc-Gwpep dmExpressionInsectMutationPromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Gwpep(Mm)-Nbr[C399-625]
Plasmid#188591PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertGW-peptide (mouse) N-terminally tagged C-terminal domain of Nibbler
UseTagsFLAG-Myc-Gwpep mmExpressionInsectMutationPromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[R272A,K276A]
Plasmid#188596PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler (Nbr Fly)
UseTagsFLAG-MycExpressionInsectMutationR272A [CGG=> GCG], K276A [AAG=>GCG]PromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
attB53_SNCB-_attB53
Plasmid#183615PurposeRMCE vector to shuttle puromycin resistance (+), anti-GFP SynNotch receptor (+), tagBFP (-) and TRE-mCherry (-) cassettes into attP50-flanked landing pad (Addgene 183609). (+) = sense (-) = antisense.DepositorInsertsLaG17 GFP nanobody SynNotch tTA
TRE-mCherry-SV40pA
tagBFP
UseRmceTagsMyc and human CD8a signal sequenceExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-human-caspase-1/4a
Plasmid#183393PurposeExpression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCARD domain of human caspase-1 fused to catalytic domain of human caspase-4 (CASP1 Synthetic, Human)
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-human-caspase-1/4b
Plasmid#183394PurposeExpression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCARD domain of human caspase-1 fused to human caspase-4 (CASP1 Synthetic, Human)
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD2604
Plasmid#175088PurposeMammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T604A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).DepositorInsertKSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
UseTagsExpressionMammalianMutationS644APromoterAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD2601
Plasmid#175085PurposeMammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions S47A, S65A, T75A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).DepositorInsertKSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
UseTagsExpressionMammalianMutationS47A, S65A, T75APromoterAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD2602
Plasmid#175086PurposeMammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T424A, T438A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).DepositorInsertKSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
UseTagsExpressionMammalianMutationT424A, T438APromoterAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD2603
Plasmid#175087PurposeMammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T504A, T508A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).DepositorInsertKSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
UseTagsExpressionMammalianMutationT504A, T508APromoterAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-OKMS-KRAB-dCas9
Plasmid#120356PurposepiggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.DepositorInsertOKMS cassette
UseCRISPR; Piggybac transposonTagsExpressionMammalianMutationKlf4 [10-483]PromoterAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Fah_S
Plasmid#163086PurposeSwingshift mutant FAH in mammalian cellsDepositorInsertsFah_S
Fah_S
UseTagsMyc-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HisFlag-rUSP19ER-CAmut
Plasmid#155237Purposefor mammalian overexpression of the mutated ER isoform of rat USP19DepositorInsertUSP19 (ER isoform) (Usp19 Rat)
UseTags6XHis-3XFlagExpressionMammalianMutationCys(545) to AlaPromoterCMVAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-GAP-ALD1
Plasmid#126711Purposeyeast plasmid complementing ALD1 from Komagataella phaffiiDepositorInsertald1
UseTags6xHis,MycExpressionYeastMutationPromoterGAPAvailable sinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-GAP-ADH3
Plasmid#126710Purposeyeast plasmid complementing ADH3 from Komagataella phaffiiDepositorInsertadh3
UseTags6xHis,MycExpressionYeastMutationPromoterGAPAvailable sinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
UQ5874
Bacterial Strain#37169DepositorBacterial ResistanceAmpicillin and kanamycinAvailable sinceAug. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
UQ5876
Bacterial Strain#37171DepositorBacterial ResistanceAmpicillin and kanamycinAvailable sinceAug. 9, 2012AvailabilityAcademic Institutions and Nonprofits only