pDD2604
(Plasmid #175088)

• Purpose: Mammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T604A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).
• Depositing Lab: Dirk Dittmer
• Publication: Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi: 10.1128/JVI.01459-21. Epub 2021 Sep 15.

Backbone

• Vector backbone: pEF1/Myc-His_A
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: KSHV RTA phospho-mutant (ORF50)
• Alt name: 188
• Species: Kaposi's sarcoma-associated herpesvirus 8
• Mutation: S644A
• Entrez Gene: ORF50 (a.k.a. HHV8GK18_gp52)

Cloning Information

• Cloning method: TOPO Cloning

Resource Information

• A portion of this plasmid was derived from a plasmid made by: WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Used genscript site-directed mutagenesis